Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"matK"”.
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Expression and Function of the Chloroplast-encoded Gene matK
The chloroplast matK gene has been identified as a rapidly evolving gene at nucleotide and corresponding amino acid levels. The high number of nucleotide substitutions and length mutations in matK has provided a strong phylogenetic signal for resolving plant phylogenies at various taxonomic levels. …
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The Phylogenetic Reconstruction of the Grass Family (Poaceae) Using matK Gene Sequences
Comparative DNA sequencing of matK, a maturase-encoding gene located within the intron of the chloroplast trnK gene, was evaluated for phylogenetic utility above the family level and within the grass family (Poaceae). There are three major objectives in the research. The first one is to study the …
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Rethinking phylogenetics using Caryophyllales (angiosperms), matK gene and trnK intron as experimental platform
… order) by comparing it with rapidly evolving matK. We show that trnK intron is comparable to matK in terms of the proportion of variable sites, parsimony informative sites, the distribution of those sites among rate classes, and phylogenetic informativness across the history of the order. This …
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Testing the utility of the consortium for the barcoding of life's two 'agreed upon' plant DNA barcodes, matK and rbcL
… of two coding chloroplast gene regions (cpDNA), matK and rbcL, would serve as the DNA barcode for all land plants. This recommendation was accompanied by CBOL‘s call for continued testing of these two regions, along with other potential gene regions that may prove to be more effective barcode …
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Development of Bioinformatic Tools and Procedures to Computationally Support DNA Barcoding
… users will be able to align the rbcL and matK barcode regions with confidence, with only a minimal amount of manual editing required for matK. The results achieved for the rbcL were roughly the same for the standalone alignment tools tested, and for the matK gene the results were better …
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Evaluating the Evolutionary and Genetic Relationships of the Andean Orchids of Northwestern Ecuador
… Ecuador. The library contains 135 <em>matK</em> and 136 <em>rbcL</em> DNA Barcodes representing over 33 Orchidaceae genera. Sequence analysis shows percent species resolution was higher for <em>matK</em> (98.8%) than <em>rbc</em>L (70.24%), with a large portion of the unresolved species …
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Applications of DNA-barcoding in the identification and understanding of grass invasions in Southern Africa
… markers for plant DNA barcoding (rbcLa + matK) were used in barcoding and phylogenetic analyses, both individually and in combination. The barcoding data was assessed for identification efficacy using three distance-based metrics and one tree-based metric in the R package SPIDER, both …
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DNA barcoding of Jatropha curcas
… carboxylase/oxygenase (rbcL) and maturase K (matK) for their ease of amplification, discrimatory power, and sequence quality in Jatropha curcas, Jatropha podagrica, Latuca sativa, and Arabidopsis thaliana. I found that the rbcL gene in J. cureas and the 3' end of L. sativa met the three …
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Impiego di tecniche innovative per la valorizzazione e caratterizzazione molecolare di specie vegetali ornamentali
… del DNA barcoding dei core markers, rbcL e matK, è stato adottato come primo passo per l identificazione di 100 taxa appartenenti a 20 famiglie. Un terzo marcatore, la regione intergenica trnH-psbA, è stato anche testato su 74 taxa, dove i core markers indicati hanno presentato …
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Molecular and Morphological Investigation of Astilbe
… data set and sequences from the plastid gene matK, (b) use single nucleotide polymorphisms to determine the lineages of cultivated varieties, and (c) successfully culture Astilbe in vitro and evaluate potential somaclonal variation of resulting Astilbe microshoots. Phylogenetic trees generated …
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Use of a Novel Combination of Multiplex PCR and DNA Barcoding in Assessing Authenticity of Ginseng Products
… three genetic targets (i.e., rbcL, ITS2, and matK). Of the three genetic markers tested with DNA barcoding, ITS2 had the greatest amplification success at 74% and matK had the greatest sequencing success at 60%. DNA barcoding alone was able to identify species in 68% of products, while …
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Evolution of the genus Aristolochia - Systematics, Molecular Evolution and Ecology
Evolution of Piperales – matK gene and trnK intron sequence data reveal lineage specific resolution contrast. Piperales are one of the largest basal angiosperm orders with a nearly worldwide distribution. The order includes three species rich genera, Piper (ca. 1,000 species), Peperomia (ca. …
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DNA Barcoding of Ayurvedic Herbal Supplements used for the Purported Treatment of COVID-19
… tested with four barcoding regions (i.e., rbcL, matK, ITS2, and mini-ITS2) using two composite samples per product. The use of the combined loci resulted in the amplification of 85.2% of the products and identification of at least one species in 64.8% of the products (based on the combined …
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Taxonomic and Phylogenetic Diversity of Pitcher Plant Bogs in Georgia's Coastal Plain
… of which 254 were sequenced for rbcL and matK gene regions of the chloroplast genome to generate a phylogeny. Sequencing returned 197 successful sequences of both rbcL and matK gene regions, totaling to 207 sequences used to construct a phylogeny after including downloaded GenBank …
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A Phylogeographic Study of Arenaria ciliata and Arenaria norvegica in Ireland and Europe
… were sequenced: psbA-trnH, rpl32 -trnL, trnK -matK and matK, and the in- ternal transcribed spacer region of the nuclear ribosomal DNA (ITS). The chloroplast and ITS sequences were analyzed using maximum likelihood, haplotype networks, and a molecular clock analysis using Bayesian inference. …
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Molekular-Systematische Untersuchungen an den Familien Nepenthaceae und Ancistrocladaceae sowie verwandter Taxa aus der Unterklasse Caryophyllidae s. l.
… Maturase K kodierenden Bereich des trnK-Introns (matK) ein Pseudogen darstellt, wurde als zus?xE4;tzlicher phylogenetischer Marker f?xFC;r die Nepenthaceae vergleichend sequenziert, eignete sich aber nur eingeschr?xE4;nkt zur phylogenetischen Rekonstruktion. Es konnte gezeigt werden, dass die hohe …
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Filogenia e evolução de estados de caráter ancestral do complexo gravisia (Bromelioideae, Bromeliaceae)
… (AGT1, ETS, phyC) e um marcador plastidial (matK), juntamente com uma reconstrução dos estados de caráter ancestrais e exploramos sua biogeografia. Nossos resultados revelaram que o complexo Gravisia não é monofilético, consistindo em uma linhagem com clados distintos que incluem táxons dos …
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The development of Deoxyribonucleic Acid (DNA) based methods for the identification and authentication of medicinal plant material.
… and H. maculatum samples respectively. The matK is currently unusable, the rbcL highly conserved, trnH-psbA problematically variable and the nrITS proved to be ideal for assay design.
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Phylogenetics of North American Psoraleeae (Leguminosae): Rates and Dates in a Recent, Rapid Radiation
… six chloroplast (trnD/T, trnL/F, trnS/G, trnK, matK, and rpoB-trnC) and two nuclear (ITS and waxy) DNA regions. This study explores approaches for coding insertion-deletion events (gaps) as phylogenetic characters using maximum parsimony and Bayesian Inference. Inclusion of gaps generally …
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Molecular systematics of the cashew family (Anacardiaceae)
… from sequences of three chloroplast loci (matK, trnLF, and rps16), using maximum parsimony and maximum likelihood as the optimality criteria. Based on these reconstructions and current knowledge of morphological and anatomical attributes of the Anacardiaceae, the subfamilies of Takhtajan, …
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