Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"mass spectrometry-based proteomics"”.
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Unravelling the molecular mechanisms of HIV associated neurocognitive disorders through mass spectrometry-based proteomics
… plausible aetiological cause. Here we have used mass spectrometry-based discovery proteomics to identify the quantitative, cell-wide changes that occur when non-transformed, differentiated human neurons are treated with HIV-Tat over time, as a novel cell culture model representing the initial …
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Analysis of membrane systems using lipoprotein nanodiscs
… and lipids with bioanalytical methods, including mass spectrometry and photonic biosensors. Mass spectrometry offers several powerful techniques for fundamental characterization of Nanodiscs and Nanodisc-solubilized membrane proteins. Native mass spectrometry delivers single-lipid resolution of …
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Live cell proximity biotinylation enables proteomic analysis of mitochondria-endoplasmic reticulum contact sites
… challenge in cell biology, and although mass spectrometry-based proteomics has made great strides in cataloging all proteins, traditional protocols are limited because they rely on a purification of the cellular entity of interest. This purification step is imperfect, and furthermore, …
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Bayesian causal inference of cell signal transduction from proteomics experiments
… Causal inference approaches applied to proteomics experiments use statistical associations between observed signaling protein concentrations to infer a causal Bayesian network model, but there is no experimental and analysis framework for applying these methods to this experimental …
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A flexible and versatile framework for statistical design and analysis of quantitative mass spectrometry-based proteomic experiments
<p>Quantitative mass spectrometry (MS)-based proteomics is an indispensable technology for biological and clinical research. As the proteomics field grows, MS-based proteomic workflows are becoming more complex and diverse. The accuracy and the throughput of the MS measurements and of the signal …
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Protein Tyrosine Phosphorylation in Haematopoietic Cancers and the Functional Significance of Phospho- Lyn SH2 Domain
… is a subject of study. Using recently developed mass spectrometry-based proteomics techniques, quantitative PTP expression and cellular pY profiles were generated for MM cell lines and mouse xenograft tumors, as well as primary AML samples. Integrated comprehensive analyses on these data …
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Biomarker Discovery in Autoimmune Diseases: A Proteomics Approach
… prime candidates for disease marker discovery. Mass spectrometry-based proteomics biomarker discovery holds the ability to interrogate a constellation of proteins simultaneously in a high-throughput manner to uncover a panel of markers that are specific to the presence of a disease. However, the …
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Proteomic Analysis of the Blood of <i>Alligator mississippiensis</i>
… focus was to investigate alligator blood using mass spectrometry-based proteomics methods to understand their innate immune systems. The first goal was to sequence peptides and proteins from the blood serum and leukocytes using tandem mass spectrometry and <i>de novo</i> sequencing. The second …
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Optimization on Single-Pot, Solid-Phase-Enhanced Sample Preparation Method for Proteomics Workflow
Mass spectrometry-based proteomics relies on effective sample preparation to achieve accurate protein identification and quantification. Among current methods, Single-Pot, Solid-Phase-Enhanced Sample Preparation (SP3) is widely used because of its simple workflow, compatibility with different …
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Optimization and Validation of the Derivation and Use of HoxB8 Transgenic Murine Phagocytes in In Vitro Studies
… cell line) via morphology, flow cytometry, mass spectrometry-based proteomics analysis, and comparison of key phagosomal parameters (phagocytic index, phagosomal maturation (intra-phagosomal proteolysis), and phagosomal NOX2 activity). I also demonstrated the genetic mutability of …
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Circadian Orchestration of the Cellular Proteome
… In the second part of this thesis I used mass spectrometry-based proteomics to characterise the cellular circadian proteome, and I uncovered the reciprocal, rhythmic regulation of protein and ion concentrations in cultured cells. Strikingly, by using a genetic knock-out of the CRYPTOCHROME …
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Development of 3D multicellular liver organoids derived from human induced pluripotent stem cells to model antiretroviral therapy induced liver injury
… cytokine levels in ART/SEA/TGZ treated HLOs. A mass spectrometry-based proteomics approach was applied to decipher protein dynamics and molecular mechanisms of ART, A+TB and TGZ -induced liver injury in treated HLOs. Results: HLOs exhibited robust mature hepatic gene markers (CYP3A4, ATA1, ALB …
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Methods to Probe the Function of Modified Bases in DNA
… mechanism in HEK293T cells. Next, mass spectrometry-based proteomics studies are utilised to determine 5-fU protein-binders in mammals. Pulldown of proteins using biotinylated baits enables the identification of proteins which are enriched or suppressed in the presence of the 5-fU …
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Homology-Based Functional Proteomics By Mass Spectrometry and Advanced Informatic Methods
… of cells and tissues. Protein identification by mass spectrometry consists of associating an isolated protein with a specific gene or protein sequence in silico, thus inferring its specific biochemical function based upon previous characterizations of that protein or a similar protein having that …
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Development and Application of a quantitative Mass spectrometry based Platform for Thermodynamic Analysis of Protein interaction Networks
… here is the development of and application of mass spectrometry-based proteomics metehods to detect and quantify the strength of protein-protein and protein-ligand interactions in the context of their interaction networks. Characterization of protein-protein and protein-ligand interactions can …
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The Proteomic Response of Northern Elephant Seal (<i>Mirounga Angustirostris</i>) Pups to Physiological Stress During Development
… post-weaning) pups. We analyzed the samples with mass-spectrometry-based proteomics using two-dimensional gel electrophoresis. Proteomics is an invaluable tool for analyzing marine mammal physiology, as it provides a large, unbiased data set of proteins that offer a comprehensive set of mechanisms …
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Tools for investigating cellular signaling networks by mass spectrometry
Mass spectrometry has become the tool of choice for proteomics. Its unrivaled coverage and reproducibility has positioned it head and shoulders above competing techniques for analyzing protein expression post-translational modification. With the increased popularity comes a flood of new research …
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Differential Lipidomic and Proteomic Responses Induced by Sub-lethal Drug Challenge in Susceptible and Drug Resistant Mycobacterium smegmatis
… to understanding the state of an organism, while mass spectrometry-based proteomics and metabolomics are powerful tools by which these can be examined. For mycobacteria in particular, the lipidome and cell wall are compartments of major importance with respect to virulence, adaptation, …
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Quantitative analysis of IL-2 and IL-15 signal transduction in T lymphocytes
… signaling pathways remain elusive. Using mass spectrometry based proteomics, we found that IL-2 and IL-15 trigger a quantitatively and qualitatively identical phosphotyrosine signaling response in T cells. The distinct effects of IL-2 and IL-15 on T cells could not be explained through …
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Characterization of D-amino acid-containing neuropeptides from metazoa
… L-form due to the chiral selectivity of ribosome-based protein synthesis mechanism. However, in recent years it has become evident that certain residues within a peptide can be inverted to the D-form in an enzyme-mediated post-translational process, forming D-amino acid-containing peptides …
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