Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 62 for “"marker genes"”.
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Agrobacterium-Mediated Transformation of Soybean: Test of Selectable Marker Genes
… transformation efficiency, three selectable marker genes, bar, ALS and ASA2 , were tested with two soybean transformation systems, e.g. cotyledonary nodes and mature embryo axes systems, with glufosinate, Arsenal and 5-MT as the selective agents, respectively. Transgenic soybean lines have …
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Machine learning enabled bioinformatics tools for analysis of biologically diverse samples
… bioinformatics tools to identify informative biomarkers from these data becomes increasingly important. These tools are crucial to extract meaningful biomarkers from this data, especially for understanding the biological pathways responsible for disease development. The identification of …
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A Molecular Dissection of the Ventromedial Hypothalamic Nucleus
… there is a paucity of nucleus-specific marker genes for the VMH that can be used to label its constituent neurons. This represents a significant impediment to the application of molecular approaches for analyzing VMH circuitry and function. Thus, we conducted a microarray screen in order …
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Generation of cDNA libraries of amoeba, 8 hour, and 12 hour stages of Dictyostelium discoideum
… Northern blot analysis and by RTPCR for several marker genes. Following cDNA synthesis, the cDNA was assessed for complexity and full-length synthesis by PCR and radioactive primer extension, respectively. Ligation of the cDNA into a vector was performed using several ratios of vector:insert in …
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The Diversity of Cortical Interneurons
… group, making it hard to classify them without markers. We took a BACtrap approach performing translating ribosome affinity purifications on transgenic mice with Bacterial artificial chromosome, for systematic discovery of markers for different cell types. First, we generated BACtrap lines for …
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Towards a complete human cell atlas: a single-nucleus RNA sequencing study of the paediatric and adult human brain
… annotated datasets to identify combinations of marker genes capable of distinguishing each cell type. Based on this, several minimal marker genes were identified which were shared between paediatric samples and not adults or vice versa. Three different tools were used to identify genes changing …
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Pareto Task Inference Analysis of Single-Cell RNASequencing of Human Placenta Reveals Biological Insightsinto Adverse Pregnancy Outcomes
… (SCTs), a sublineage of trophoblasts. Marker genes and gene set enrichment analysis for the ParTI polytope vertices, called archetypes, were performed to assess the biological states associated with the archetypes. We demonstrated that the ParTI polytope can separate both broad cell …
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The Role of MicroRNA-378a-5p and its Target Genes in Differentiation and Fusion of BeWo Cells
… the role of miR-378a-5p and its target genes in STB differentiation by using a choriocarcinoma cell line, BeWo. Using luciferase reporter assays, we showed that miR-378a-5p targets CREB. Transfection of miR-378a-5p decreased, while antimiR-378a-5p increased CREB protein and mRNA levels. …
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Study of anti-cancer effect of winter worm and summer grass on Mcf-7 human breast cancer cells
… by inducing the expression of pro-apoptotic genes and by suppressing anti-apoptotic marker genes. Moreover, the C. militaris extract also inhibits DNA methyltransferase transcription, suggesting that the reduced cancer suppressor gene methylation might lead to the recovery of tumor-suppressor …
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Head and protocerebral patterning in the spider Parasteatoda tepidariorum
… of gene expression patterns during spider embryogenesis. To understand the regulation of the development of the spider pre-cheliceral region, cheliceral and pedipalp segments of the spider embryo, I first analysed marker genes in five putative anterior cell clusters from scRNA-seq analysis at …
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A Novel Click Chemistry-Based Method to Detect Hypoxic Tumour Cells and Characterise Their Gene Expression
… cells, the ‘clickable’ 2- nitromidazole hypoxia marker (SN33267) bearing a terminal alkyne group can be derivatised via copper(I)-catalysed alkyne-azide cycloaddition (CuAAC) with azides. Labelling of hypoxic cells with fluorophore azides, after exposure to SN33267, was optimised in vitro by …
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Understanding tumor cell plasticity in spatial transcriptomics with graph attention networks and walk-based pseudotime analysis
… states despite a limited set of tumor marker genes. This cellular differentiation path shows enrichment and depletion of certain cell types within local neighborhoods aligning with known correlations, and by leveraging inferred ligand-receptor interactions, we can pinpoint potential …
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Transformation of a Transposon Construct into Tomato for Functional Genomics Studies
… a transposon from maize that can be used for mutagenesis when it transposes or moves around the genome of the tomato. The vector used is a two-component in-cis Ac-Ds system which needs a single transformation event. It was proved that it worked in Arabidopsis and rice according to Trijatmiko …
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Single cell landscape of innate and adaptive immunity in metastatic melanoma treated with immunotherapy
… experience a durable response. To find better biomarkers for ICI response and understand the diverse cellular players in the tumor, we performed a multi-omic study on a metastatic melanoma cohort with RNA sequencing (39 samples), single cell RNA sequencing (222,351 cells; 39 samples), and single …
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Mechanotransduction Modulates the Effect of Mechanical Forces (Fluid Shear Stress and Cyclic Strain) on Embryonic Stem Cell Differentiation Toward Vascular Endothelial Cells
… <p>In the early stages of embryonic vasculogenesis, blood islands formed by the hemangioblasts give rise to the early vasculature needed to nourish the embryo. At this stage, fluid shear stress of blood flow is the principal mechanical force that drives further differentiation into …
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Mechanisms of Erythroid Proliferation and Differentiation: Analysis of the Role of Erythropoietin Receptor in the Friend Virus Model
… by exiting cell cycle and expressing erythroid marker genes, which is independent of its established role in survival. More importantly, FVA cells lacking the STAT5 activation fail to undergo terminal differentiation in response to EPO. Taken together, our data support a novel instructive …
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Engineering Tools to Promote and Characterize Wnt-Mediated Stem Cell Differentiation
… to detect the expression of Wnt-regulated marker genes with high specificity. Luminescent signals produced with the help of luciferase secreted by these cell lines during directed differentiation into cardiomyocytes permit continuous non-destructive monitoring of the manufacturing process. …
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Host Specific Microbial Source Tracking and Quantification Using the Partial 16SrRNA Gene Sequence of Bacteroides on the Wilson's Creek Tributary in Southwest Missouri
… assay using Bacteroides host-associated 16SrRNA marker genes was used, along the with Colilert E. coli MPN enumeration method for this survey. There were multiple occasions at each site where the E. coli MPN exceeded the Missouri DNR recommended safety standard of 126MPN/100ml. Bovine-associated …
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Towards dissecting neural ensembles : development of genetic profiling and targeting approaches
… sense expression levels of cell-type specific marker genes and compute complex Boolean logic to label these cells specifically. As a proof of principle, we demonstrate that the synthetic circuit achieves classification of two cell lines based on their gene expression profiles with high …
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