Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 6 of 6 for “"manXYZ"”.
-
Regulation of the Mannose PTS Operon by the small RNA SgrS
… the mannose and secondary glucose transporter, manXYZ. Analysis of manXYZ mRNA stability and translation in the presence and absence of SgrS indicate that manXYZ is regulated by SgrS under stress conditions and when SgrS is ectopically expressed. In vitro footprinting and in vivo mutational …
-
Mechanistic and physiological insights into post-transcriptional regulation by small RNAs SgrS and DicF
… was previously shown to negatively regulate the manXYZ broad sugar-substrate transporter. In this study we demonstrate that SgrS binds at manX and in the intergenic region of manXY to translationally silence this operon. We show that pairing at both these sites is critical for degradation of the …
-
Sweet relief: regulating the response to glucose phosphate stress by the small RNA SgrS & Co.
… translation of sugar transporter mRNAs (ptsG and manXYZ) and enhances translation of a sugar phosphatase mRNA (yigL). Despite a molecular understanding of individual target regulation by SgrS, previously little was known about how coordinated regulation of these targets contributes to the rescue …
-
Molecular analysis of small RNA and small protein regulation of Escherichia coli stress responses
… major and minor glucose transporters PtsG and ManXYZ, thereby inhibiting synthesis of additional glucose permeases and restricting further influx of non-metabolizable sugars. In this study we demonstrate that SgrT acts to specifically inhibit the transport activity of preexisting PtsG …
-
An E. coli small RNA inhibits translation initiation from a distance
… In the targetome of an E. coli sRNA SgrS, manXYZ is a non-canonical target where SgrS base pairs at two distinct sites that are far from the SD regions of manX and manY, yet translation of these two cistrons are repressed by SgrS. We found that manX translation is controlled by a molecular …
-
Target regulation and prioritization by the small RNA SgrS in Escherichia coli
… pairs with and represses translation of ptsG and manXYZ mRNAs, which encode sugar transporters, and activates translation of yigL mRNA, encoding a sugar phosphatase. In this study, transcriptomic analyses along with genetics and biochemistry defined four new direct targets of E. coli SgrS. These …