Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 54 for “"mammary tissue"”.
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Predicting post-absorptive amino acid supply to the mammary tissue
… current models. A greater supply of AA to the mammary glands does not always mirror AA absorption—the process by which absorbed AA convert into milk protein is variable, and this may be linked to the way the udder regulates AA uptake to preserve intracellular balance. AA transporters housed …
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Histological evaluation of ovine mammary tissue xenografted into cyclosporine treated mice
… in the third), were implanted with ovine mammary tissue expants at various sites (subcutaneous dorsum, ovarian fat-pad, and cleared mammary gland fat-pad). After different periods of time, (day 3, 7, 14, 21, and 35) implants were removed, fixed, stained, and embedded. Epithelial morphology …
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Histology of bovine mammary tissue during advanced stages of induced lactation
… on days 18, 19 and 20 after initial treatment. Mammary tissue biopsies were taken from 9 cows on days 18, 21 and 23 after initiation of treatment (3 cows each day). Histological analysis was performed (> 30,000 contacts) to determine the average percent tissue area composed of epithelium, …
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Essential Amino Acid Regulation of Cell Signaling and Casein Synthesis in Mammary Tissue
… initiation and to stimulate protein synthesis in mammary tissue. The<br />objectives of this research were to determine the response to Ile, Leu, Met, and Thr in<br />cellular signaling and "-S1 casein fractional synthesis rates (CFSR). An experiment was<br />developed as a composite design. The …
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Abundance and Localization of (Yes-associated protein) YAP in Prepubertal Bovine Mammary Tissue
Most mammary development is postnatal. Mammary growth that occurs before puberty is diminutive in amount but consequential for future milk production, especially in dairy heifers. With advanced knowledge on fundamental aspects that govern prepubertal mammary development, scientists and farmers …
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A quantitative study of the morphology of normal, hyperplastic, and neoplastic mammary tissue in the mouse
… histological account of two aspects of mammary gland morphology. First, the normal mouse during different functional phases including inactivity, pregnancy, lactation and involution is presented. Second, the abnormal changes of mammary hyperplasia and neoplasia - both spontaneous and …
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In vitro milk protein secretion by explants of Holstein bull mammary tissue from two different genetic lines
The ability of mammary tissue from mature Holstein bulls to respond to hormonal stimuli in organ culture to synthesize and secrete milk proteins was studied. Sixteen bulls from the genetic selection and control lines developed at Virginia Polyteclmic Institute were randomly assigned to either a …
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Peptides as amino acid sources for the synthesis of secreted proteins by mammary tissue explants and cultured mammary epithelial cells
… for the synthesis of secreted proteins. Mammary tissue explants from lactating (10 to 11 d) CD-1 mice and cultured bovine mammary epithelial cells (MAC-T) were used as experimental models. Explants and cultured cells were incubated at 37°C in a humidified atmosphere of 90% air/10% CO₂ and …
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Purification of Calmodulin and Quantification of Its Levels in Mammary Tissue and Isolated Cells During Pregnancy and Lactation (Acini, Secretory, Myoepithelial)
… to apparent homogeneity from lactating bovine mammary tissue. Calmodulin was purified by heat treatment and sequential anion exchange and gel exclusion chromatographies. The molecular weight of the purified protein was estimated by gel electrophoresis to be 18,000 daltons. The purified protein …
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Influence of hormones on synthesis and secretion of milk proteins by mammary tissue from male and female cattle of beef and dairy breeds
The ability of mammary tissue from prepubertal bulls and heifers of beef and dairy breeds to respond to hormonal stimuli through synthesis and secretion of milk proteins was studied. Experimental animals were six to eight month old Angus and Holstein cattle. All subjects were injected with …
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The Role of Prolactin in CCL28 Regulation
… breast milk. B cells that produce IgA enter the mammary tissue during lactation and secrete IgA into the milk. During pregnancy, the mammary tissue expresses high levels of chemokines, molecules that allow lymphocytes to selectively home to specific tissues. The chemokine CCL28 has been shown to …
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Aspects of lactation endocrinology: I. lactogenic receptors in bovine mammary tissue at different stages of lactation: II. growth hormone concentrations in Holstein cattle of differing genetic merit
Mammary tissue from nine Holstein cows was collected within one week of parturition, at 60 and 180 days postpartum. Blood samples were collected at 6-hr intervals from two days prior to . until two days after surgery. A membrane-enriched fraction of tissue homogenates was prepared by differential …
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Stearoyl-COA Desaturase Gene Transcription, mRNA, And Activity In Response To Trans-Vaccenic Acid And Conjugated Linoleic Acid Isomers
… the 9/11CLA content of carcass, liver, and mammary tissue of lactating mice. A similar enhancement of 9/11CLA also was observed in liver, but not carcass, of suckling pups nursing TVA-fed dams. The CLA mixture decreased mammary acetyl-CoA carboxylase (ACC) activity compared with other …
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Effects of bodyweight and plane of nutrition on mitogenic capacity of mammary extracts in cell culture, mammary growth and development, and protein expression profiles of mammary tissue in Holstein heifers
Mammary gland samples from a large serial-slaughter Holstein heifer nutrition trial were used to determine the effects of stage of development and nutritional management on mitogenic activity of mammary extracts and mammary parenchymal composition. Stage of development and nutritional management of …
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Innate Immune Cells may be Involved in Prepubertal Bovine Mammary Development
Pre-pubertal bovine mammary development involves ductal and stromal tissue changes. In mice, this process is impacted by presence of innate immune cells. Whether or not such immune cells are present or involved in bovine mammary development is unknown. We studied the presence, location and changes …
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Isolation and Characterization of Fatty Acid Binding Protein From Lactating Bovine Mammary Gland
… had been found in a wide range of organisms and tissues. Its presence had not been documented in mammary tissue. The purpose of this study was to determine whether fatty acid binding protein (FABP) exists in bovine mammary tissue and if so, to isolate and characterize it. Mammary tissue was …
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The Role of Insulin-like Growth Factor-I and IGF-binding Proteins in Mammary Gland Development
Development of the mammary gland is likely mediated by locally produced growth factors acting in concert with circulating mitogens. To investigate the importance of mammary synthesis of insulin-like growth factor-I (IGF-I) and IGF-binding proteins (IGFBP), the initial objective was to evaluate the …
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Characterization of Dendritic Cells in the Bovine Mammary Gland
… in-vitro, little is known about DC in mammary tissue. In this study, immunohistofluorescence was used to identify and localize bovine mammary DC. DC were found in alveoli, in epithelia, and in interalveolar tissue. Fluorescence-activated cell sorting (FACS) was used to characterize …
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Local regulation of increased milk yield due to early lactation increased milking frequency
… 5 (IGFPB5), or chitinase 3-like-1 (CHI3L1) in mammary tissue at d 21 or 60; and no difference in protein expression of STAT5, Akt, or STAT3 in mammary tissue at d 60.
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