Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 33 for “"mammary epithelial cell"”.
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FUNCTIONAL STUDY OF MAMMARY EPITHELIAL CELL ARCHITECTURE
Cell organization confers cellular identity and guides cellular functions. Here, the
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Proteomic Analysis of Mammary Epithelial Cell Development
… used to investigate the protein profile of the mammary epithelial cell (MEC) through different stages of mammary development. The HC11 cell line was used to investigate protein changes between undifferentiated and differentiated MEC, which represent the pregnant and lactating states of the …
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Three-Dimensional Scaffolds for Mammary Epithelial Cell Growth
Mammary epithelial cells are highly efficient secreting cells. This natural phenomenon has triggered research efforts to focus on using these cells to assist in the production of viable liquids. The application of this technology is nearly endless. If developed successfully, mammary epithelial …
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The inhibition of mammary epithelial cell growth by the long isoform of Angiomotin
Mammary ductal epithelial cell growth is controlled by microenvironmental signals in serum under both normal physiological settings and during breast cancer progression. Importantly, the effects of several of these microenvironmental signals are mediated by the activities of the tumor suppressor …
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Modeling mammary epithelial cell polarization and the role of podocalyxin in breast tumor progression
The mammary gland consists of an organized network of epithelial ducts and lobules. This histoarchitecture can be recapitulated in vitro by culturing mammary epithelial cells as 3D spheroids embedded in a reconstituted basement membrane. I first used this assay to characterize the role of cell-cell …
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Effects of low linolenic soy oil on pre-malignant human mammary epithelial cell progression
… athymic mice received human pre-malignant breast cells (MCF-10AT1 1 x 105 cells/40μl/ Matrigel/spot, 4 spots/mouse). Mice were divided into three groups and then fed isocaloric and isonitrogenous diets with disparate fat sources: LLSO (20% of total energy intake), SO (20%) and lard (20%). The …
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Regulation of Bovine Mammary Epithelial Cell Response by Autocrine IGF-I and by Collagen I
… growth factor-I (IGF-I) signaling in mammary epithelial cells may be modified or interrupted by modifications in the cellular environment may lead to 1) methods to increase the growth and proliferation of normal mammary epithelial cells for an increase in the amount of milk produced on …
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Microphysiometry Studies of Rapid Binding of Insulin-Like Growth Factor I by Parental and Transfected Mammary Epithelial Cell Lines
… uncontrolled proliferation of breast cancer cells. Insulin-like growth factor-I (IGF-I) is known to be a potent mitogen for mammary epithelial cells. IGF-I acts by binding to cell surface receptors, thereby stimulating a cascade of events leading to cell division. In the interest of …
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Effects of ovarian steroids on bovine mammary epithelial cells: in vitro and in viro evidence of indirect stimulation of proliferation
… the effects of ovarian steroids on bovine mammary epithelial cell proliferation. In a first study, estrogen (E), progesterone (P), or E+P were administered to prepubertal beef heifers and biopsied mammary parenchyma taken before and following treatment were compared for growth by evaluation …
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Effects of Growth Hormone and Insulin-Like Growth Factor-I on Milk Protein Gene Expression and Nutrient Uptake and Cell Proliferation in Clonal Bovine Mammary Epithelial Cells
… a major mediator of GH action in vivo, on cell proliferation, nutrient transport, and milk protein gene expression in bovine mammary epithelial cell line MAC-T cells were determined. GH increased (P < 0.01) expression of four major milk protein genes in MAC-T cells transfected with GHR …
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Influence of Dietary Protein Intake on the Molecular Aspects of 7,12-Dimethylbenz(a)anthracene Induced Mammary Carcinogenesis (Cancer, Liver)
… (DMBA) by their isolated mammary epithelial cell aggregates was examined. DMBA was metabolized to bay-region anti- and syn- dihydrodiol-epoxides that bound to deoxyadenosine and deoxyguanosine of mammary cell DNA. During the initial 24 hours of incubation of the mammary …
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A Proteomic Analysis of Differentiation in the Mammary Epithelium
… and the structural development of the mammary gland from pregnancy to lactation, very little is known about the molecular mechanisms governing differentiation of the mammary epithelium into a milk-secreting phenotype. It is important to acknowledge the diversity among the mammary glands …
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Xenogeneic transplantation of immortalized bovine mammary epithelial cells
… to investigate the use of an immortalized bovine mammary epithelial cell line as a starting material for xenogeneic transplantation into the mammary glands of immunocompetent recipients. PSG-5 cells (a clonal derivative of the MAC-T cell line engineered to express ovine IGF-I ) were transplanted …
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Pharmacological regulation of c-myc gene expression in human breast cancer cells
… studies demonstrated that quinidine causes G1/G0 cell cycle arrest and inhibition of proliferation in MCF-7 human breast cancer cell line (Woodfork, K. et al., 1995). The goal of studies reported here was to understand the molecular mechanisms of c-myc gene regulation by quinidine. C-myc is one of …
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Cancer Chemoprevention by Curcumin and Structurally Related Beta-Diketones
… of curcumin to act as an inhibitor of mammary carcinogenesis needs further attention. The present studies were conducted in order to evaluate curcumin, and structurally related compounds, to act as effective blocking agents toward the initiation stage of chemically induced …
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In Vitro Binding and Transport Regulation by Endothelial Cells: Preliminary Studies looking at FIX and IGF-I
Endothelial cells separate the bloodstream from the underlying tissue and play a crucial role in vascular homeostasis. They also form an important barrier for vascular drug delivery. This thesis contains preliminary studies targeted at understanding the mechanisms of binding and transport across …
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Investigating milk cell population changes in response to heat stress and characterizing functionality of epithelial cells in milk of lactating dairy cows
Understanding the number of mammary epithelial cells a cow maintains is essential to maximize milk synthesis in lactating dairy cows. This thesis aimed to investigate milk cell population changes in lactating dairy cows in response to heat stress (HS) using a pair-feeding model (PFTN). …
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Micro lipid droplet precursors of milk lipid globules
… derived from the apical plasma membrane of the mammary epithelial cell during secretion. In lactating mammary epithelial cells, immediate precursors of milk lipid globules appear to be cytoplasmic lipid droplets (CLD). These cytoplasmic lipid droplets have diameters >1 μm and are characterized …
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Systemic and Intracellular Trafficking of Long-chain Fatty Acids in Lactating Dairy Cattle
… patterns in the small intestine and to compare mammary uptake. Milk transfer of fatty acids delivered as intravenous triglyceride emulsions was reduced with increased chain length and unsaturation. Increased target fatty acids were evident in plasma phospholipid, suggesting re-esterification in …
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New methods to study human mammary development and breast cancer
… arise from within the normal architecture of the mammary gland and resemble normal mammary epithelial cell types on a molecular and gene expression level. Many tumors become dependent on the signaling pathways that guide mammary differentiation and proliferation, and may be driven by transcription …
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