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Showing 1 to 20 of 24 for “"mammary cells"”.
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Cultivation and Biochemical Characterization of Bovine Mammary Cells in Tissue Culture
Made available in DSpace on 2014-12-05T23:07:57Z (GMT). No. of bitstreams: 1 6003908.pdf: 4715362 bytes, checksum: 3127100714074edf2418c5bc96746d8a (MD5) Previous issue date: 1960
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Extracellular amino acid effects on milk protein synthesis and free amino acid pools in cultured rat and bovine mammary cells
Mammary cells from lactating rats and dairy cows were cultured in Eagle's minimal essential medium (MEM) with added amino acids. Changes in free intracellular amino acid pools and milk protein synthesis in response to amino acid additions to the medium were measured. Increases in free intracellular …
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Influence of in vitro elaidic acid or trans-vaccenic acid uptake and lactogenic hormone stimulation on fatty acid content of mouse mammary cells
… and alteration of exogenous fatty acids by mouse mammary epithelial cells. Cells from a subclone of the COMMA-D cell line were plated on uncoated plastic petri dishes and grown to confluence. Supplemental fatty acids bound to bovine serum albumin were added to the medium applied to the confluent …
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Influence of Dietary Protein Intake on the Molecular Aspects of 7,12-Dimethylbenz(a)anthracene Induced Mammary Carcinogenesis (Cancer, Liver)
… (DMBA) by their isolated mammary epithelial cell aggregates was examined. DMBA was metabolized to bay-region anti- and syn- dihydrodiol-epoxides that bound to deoxyadenosine and deoxyguanosine of mammary cell DNA. During the initial 24 hours of incubation of the mammary …
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In Vitro Study of an Orange Oil Derived Alternative to Antibiotics in the Treatment of Bovine Mastitis
… aureus growth and invasion of bovine epithelial mammary cells, but only modest reductions in pre-formed biofilms, which contribute to persistence of S. aureus infections. Our second study examined major components of terpeneless, CPV orange oil. Of four major compounds tested, only citral and …
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Three-Dimensional Scaffolds for Mammary Epithelial Cell Growth
Mammary epithelial cells are highly efficient secreting cells. This natural phenomenon has triggered research efforts to focus on using these cells to assist in the production of viable liquids. The application of this technology is nearly endless. If developed successfully, mammary epithelial …
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Transcriptional regulators in stem cell biology
Adult stem cells support tissue regeneration by both self-renewing and differentiating into specialized cell types. Analogous self-renewal and differentiation processes enable cancer stem cells to promote tumorigenesis. Transcriptional modulation, enacted by proteins including epigenetic regulators …
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Determination of optimum amino acid complement for milk protein synthesis in the dispersed rat mammary cell culture
… protein synthesis was investigated in the rat mammary cells in tissue culture. Studies involved: 1) the amino acid reduction from a positive control (3X) back to a negative control (1X) for each of the essential amino acids that were present in the 1X-MEM (Omission Study) [X refers to the amino …
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Stearoyl-COA Desaturase Gene Transcription, mRNA, And Activity In Response To Trans-Vaccenic Acid And Conjugated Linoleic Acid Isomers
… the 9/11CLA content of carcass, liver, and mammary tissue of lactating mice. A similar enhancement of 9/11CLA also was observed in liver, but not carcass, of suckling pups nursing TVA-fed dams. The CLA mixture decreased mammary acetyl-CoA carboxylase (ACC) activity compared with other …
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Insulin Recepto Loss Impairs Mammary Tumourigenesis in Mice
… directly probe the role of insulin signaling in mammary tumorigenesis, we generated the MMTV-driven polyoma middle T (PyMT) and ErbB2/Her2 BC mouse models, respectively, with coordinate mammary epithelium-restricted deletion of the INSR. In both models, deletion of either one or both copies of …
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SOMATIC MUTATIONS AT FRAGILE ENHANCERS OF LINEAGE- IDENTITY EPITHELIAL GENES IN BREAST CANCER.
… that double-strand breaks (DSBs) in normal mammary cells are not randomly distributed, but cluster at specific sites including gene promoters. Our lab identified paused RNA polymerase II and topoisomerase2B as key contributors to DSB formation at specific promoters, and further demonstrated …
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Hypoxia and Hypoxia-Inducible Factors in Normal and Malignant Breast Epithelium
… set out to explore the impact of hypoxia on mammary epithelial differentiation and what consequences the hypoxic response might have on breast cancer development and behavior. We found that hypoxia induces a less differentiated, estrogen receptor (ER)-negative/cytokeratin (CK) 19-positive …
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CARATTERIZZAZIONE IN VITRO DELLA RISPOSTA A STIMOLI INFETTIVI DELLE CELLULE EPITELIALI MAMMARIE BOVINE (BME-UV)
In vitro assessment of bovine mammary epithelial cells response to infectious stimuli Non-specific (innate) immune response plays a major role in defending the udder from bacterial invasion. Moreover, recent investigations suggest that mammary gland epithelial cells could have a large and important …
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DNA methylation in the early porcine embryo
… and in vitro culture of early embryos and donor cells for somatic cell nuclear transfer has been shown to have detrimental effects on the epigenetic factor of cytosine methylation in cytosine-guanine dinucleotides. The purpose of this research is to study the dynamics of DNA methylation in …
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Tumor Suppression by a TRNA Synthetase
… women. During malignant transformation, healthy mammary cells must bypass tumor suppressive checkpoints and activate pro-growth pathways. Enhanced protein translation is one such hallmark of tumor transformation and cancer progression; many oncogenes promote translation by driving synthesis and …
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Regulatory Roles of Essential Amino Acids, Energy, and Insulin in Mammary Cell Protein Synthesis
… with insulin, glucose and acetate on mammary protein synthesis rates, 2) to investigate whether branched chain amino acids (BCAA): leucine , isoleucine , and valine , become limiting for milk protein synthesis when Met and Lys supply were not limiting, and 3) to develop a mathematical …
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Extracellular Proteoglycan Decorin in Bovine Mammary Physiology
The majority of bovine mammary gland research focuses on the main cell types - mammary epithelial cells and fibroblasts. However, the extracellular matrix (ECM) within the mammary gland is also of importance for its ability to regulate cell shape, proliferation, polarity, differentiation, gene …
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Polymorphic Variation of the DGAT1 Gene in Cal Poly Holstein and Jersey Breeds and the Correlation of Milk Fat Content, and Genetic Values
… to examine the DGAT1 expression in epithelial mammary cells. These live epithelial cells were obtained via the novel technique of magnetic separation, which had questionable results. Microscopic evidence shows binding has limited success, and the inconclusive correlation between DGAT1 genotype …
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Activity and mRNA abundance of enzymes for fatty acid synthesis and desaturation in mammary cell cultures
… acids on cellular fatty acid biosynthesis in mammary cells was examined. Under normal situations, even though the diet of a dairy cow contains considerable amounts of unsaturated fatty acids, viz. oleic acid (18:1) and linoleic acid (18:2), the major 18-carbon fatty acid that enters the …
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Gene Set Analysis of Post-Lactational Mammary Gland Involution Gene Signatures In Inflammatory and Triple Negative Breast Cancer
… each cycle of pregnancy and lactation, the mammary gland undergoes dramatic changes. Clarkson et al. (2004) and Stein et al. (2004) conducted a detailed analysis of these changes in the mouse mammary gland. Their results showed that tissue remodeling during post-lactational involution period …
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