Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 47 for “"mammalian development"”.
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DNA methylation in early mammalian development
… DNA methylation profiles across pre-implantation development to identify the targets and characterize the dynamics of global demethylation that lead to totipotency and the subsequent changes to embryonic specification. In Chapter 1, I validate and refine the decades old model for DNA methylation …
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The functional microRNA landscape of mammalian development
… generation from longer precursor transcripts, mammalian miRNAs direct posttranscriptional repression of mRNAs based on complementarity to sites in their 3' UTRs. To better understand how these regulators impact fundamental processes such as development and cancer, we explored the functional …
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Discovery of miRNA-regulated processes in mammalian development
… in 3'UTRs suggest that over half of genes in mammalian genomes are miRNA-regulated. Many miRNAs are also ubiquitously expressed in a variety of embryonic and adult tissues. Given these observations, we have sought to address the challenge of determining which cellular and biological processes …
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Exploring the regulatory roles of microRNAs in mammalian development
… miRNA populations in three distinct murine developmental programs, T lymphocytes, embryonic stem cells, and the placenta. We started exploring the role of miRNAs in T lymphocytes by globally characterizing short RNA expression during key developmental stages of T lymphocytes. Our results …
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The Role of Target-Directed MicroRNA Degradation in Mammalian Development
Pages vi-xvi are misnumbered as pages vii-xvii.
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The Regulation of Dna Methylation In Mammalian Development and Cancer
… in the regulation of DNA methylation during mammalian development and in cancer. Using mouse embryonic stem cells (mESCs), an ideal model system for studying DNA methylation, I have discovered novel regulatory mechanisms that play important roles in de novo and maintenance DNA methylation. In …
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Exploring functional significance of asymmetric DNA methylation in early mammalian development
… It is also crucial for correct embryonic development and the differentiation of embryonic stem cells (ESCs) into diverse cell types. Although in mammals DNA methylation occurs predominantly in the symmetric CG context, it has been shown that certain cell types and tissues (ESCs, oocytes, …
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Fanconi Anaemia DNA crosslink repair factors protect against LINE-1 retrotransposition during mammalian development
… such events. Finally, I find that early zygotic development is particularly dependent upon FA ICL repair factors to prevent LINE-1 retrotransposition.
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First evidence of skelemin, a myosin-associated protein, in smooth muscle and its involvement in cell adhesion, and, The role of the cell cycle in cell type choice during mammalian development
… of the cell cycle in cell type choice during mammalian development. Discussed are methods employed to improve resolution for accurate analysis.
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A lentiviral system for RNAi transgenesis and the Ena/VASP triple-knockout defines neuronal and non-neuronal functions in mouse development
Mammalian development extends and exploits signaling pathways that function exclusively in axon guidance in lower organisms. This emerging paradigm employs complex expression patterns of expanded protein families to achieve the complexity and specificity required in mammalian development. For …
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Regulation of Transcription Through RNA Polymerase II Promoter-Proximal Pausing
… for cells to respond to external stimuli and for mammalian development, and that dysregulation of this network has been seen in developmental and immunological disorders, heart disease and a variety of cancers. However, what are the molecular and functional roles of Pol II pausing during normal …
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The role of Polycomb-mediated epigenetic regulation in embryonic stem cell differentiation
During mammalian development, a single founding cell must produce all of the different types of cells in the adult organism. What are the regulatory mechanisms required to coordinate the necessary gene expression networks for this process? Polycomb group (PcG) proteins are epigenetic regulators …
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Role of small RNAs and chromatin in transposable element silencing during global demethylation
… (TEs). DNA methylation is dynamic during early mammalian development, despite being a more static mark in somatic cells. Global hypomethylation is a hallmark of epigenetic reprogramming in mammalian primordial germ cells (PGCs), the early embryo and in naïve embryonic stem cells (ESCs). Genome …
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Genomic analysis of control of cell type
In mammalian development, a single fertilized egg grows into a complex organism, comprised of organs and tissues made up of hundreds of different specialized cell types. All of these cells contain the same genome, but express distinct sets of genes and proteins, which give the cells their …
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Investigation of Ring Finger Protein 8 in Mammalian Physiology and Pathogenesis
… to the maintenance of a stable cellular genome. Mammalian cells have evolved a sophisticated DNA damage response (DDR) network that senses the physical existence of DNA DSBs, followed by signal propagation to repair these genetic lesions. Interestingly, DSBs are not only hazardous, but are also …
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Roles for chromatin regulators during differentiation of embryonic stem cells
Mammalian development involves the process by which a single fertilized egg develops into an adult with over 200 specialized cell types, each with a distinct gene expression pattern controlling its cellular state. As cells differentiate into specialized cell types, changes in the gene expression …
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Molecular Characterization of Mammalian De Novo DNA Methyltransferase Chromatin Recruitment
… deposition of CpG methylation is critical for mammalian development and tissue homeostasis and is often dysregulated in human diseases. The genomic localization of de novo DNA methyltransferases 3A (DNMT3A) and 3B (DNMT3B) is facilitated in part by chromatin "reader" domains to establish DNA …
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Cross-species single-cell transcriptomics and its applications in evolutionary and translational research
… to compare gene expression, cell types and developmental programmes across organisms and interpret these transcriptomic observations within a phylogenetic framework. This opens up new possibilities to study the evolution of cell types and infer molecular properties of organisms inaccessible …
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E2F6 in axial skeletal development and gliosis
… novel roles for these proteins in different developmental processes. This dissertation examines the biological role of E2F6 in mammalian development. E2F6 functions as a repressor of transcription in concert with the polycomb group (PcG) proteins and chromatin modifiers. PcG proteins regulate …
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The role of the PWWP domain in the DNA methyltransferase 3A targeting to the genome
All cells in a mammalian body share the same DNA content but have a distinct appearance and function. This is achieved by activation and repression of genes by epigenetic mechanisms, which are crucial for stable differentiation of cells in a multi-cellular organism. DNA methylation, established by …
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