Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 570 for “"mammalian cells"”.
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Characterisation of HP1γ in mammalian cells
… as other DNA-dependent biological pathways. The mammalian HP1 (Heterochromatin protein 1) protein family consists of three members: HP1α, β and γ. Each paralogue regulates formation and maintenance of heterochromatin by binding to the repressive chromatin marks H3K9me2/3 with their chromodomains …
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Genetic Code Expansion in Mammalian Cells
… precision and minimal perturbation. Cultured mammalian cells present an attractive model for studying human biology and disease. The first aaRS/tRNA pair used for genetic code expansion in mammalian cells was the Escherichia coli (Ec) tyrosyl-tRNA synthetase (TyrRS) and Bacillus …
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Discontinuous DNA synthesis in mammalian cells.
Thesis. 1976. Ph.D.--Massachusetts Institute of Technology. Dept. of Biology.
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Continuous Directed Evolution in Mammalian Cells
… evolved biomolecule is intended to function in mammalian cells. As a result, the functions of biomolecules evolved in these environments are often derailed in the complex mammalian cellular environment. The development of highly efficacious methods for directed evolution in mammalian cells has …
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Investigating Mechanotransduction and Mechanosensitivity in Mammalian Cells
… are made up of a multitude of individual cells that are surrounded by biomolecules and fluids. It is well known that cells are highly regulated by biochemical signals; however it is now becoming clear that cells are also influenced by the mechanical forces and mechanical properties of the …
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DISCRIMINATION OF LIPOSOME-DELIVERED RNA BY MAMMALIAN CELLS.
DISCRIMINATION OF LIPOSOME-DELIVERED RNA BY MAMMALIAN CELLS.
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Characterization of ceramide synthases (Cers) in mammalian cells
… of ceramide (Cer) biosynthesis by mammalian cells. The possibility that Cer undergo developmental changes was explored using mouse embryonic stem cells versus embryoid bodies by analysis of the Cer subspecies by liquid chromatography, electrospray ionization-tandem mass spectrometry …
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Telomere Dynamics and End Processing in Mammalian Cells
… overhangs. With each cell division, normal human cells lose a small amount of telomeric DNA due to the end-replication problem and the action of an unidentified nuclease. In order for tumor cells to divide indefinitely, they maintain telomere length by expressing the enzyme telomerase. The end …
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SCAP, Insig, and Cholesterol Interactions in Mammalian Cells
Cholesterol synthesis in mammalian cells is highly regulated by an end-product feedback mechanism. The transcription of genes necessary for both fatty acid and cholesterol production are controlled by sterol regulatory element binding proteins (SREBPs). The critical regulatory step is the …
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Repair of DNA-protein crosslinks in mammalian cells
… to study the repair of DNA-protein crosslinks in mammalian cells. DNA-protein crosslinks (DPCs) are bulky lesions which disrupt important cell processes such as transcription and replication. They are formed by endogenous molecules such as formaldehyde and exogenous damaging agents such as …
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Adenovirus-based exogenous gene expression in mammalian cells
… as a model to study exogenous gene expression in mammalian cells. More specifically, several adenoviral components were identified to enhance gene expression together with components needed for viral DNA replication. The adenoviral elements that enhance gene expression were assembled in an …
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Models of dynamic RNA regulation in mammalian cells
… the cellular circuits that control RNA levels in mammalian cells, focusing on the model response of immune dendritic cells to pathogens, through an integration of comprehensive computational models and innovative empirical approaches. I establish a computational framework to follow the dynamics of …
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Translational regulation by short RNAs in mammalian cells
The large complexity of animals is thought to depend upon the regulation of gene expression and not the number of genes in a genome. Gene expression is a highly conserved process in which genes encoded by DNA are transcribed in the nucleus into messenger RNA, spliced, and exported to the cytoplasm, …
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Optimizing Zinc Finger Nucleases for Use in Mammalian Cells
… rate of homologous recombination in human cells is too low (10-6) to be therapeutically useful. The most powerful way of stimulating homologous recombination is by introducing a double strand break within the target locus. Zinc Finger Nucleases (ZFNs) are designed proteins that fuse a zinc …
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Consequences of partial chromosome re-replication in mammalian cells
… geminin in synchronised human U2OS cells are investigated in this<br/>PhD Thesis to elucidate the possible contribution of re-replication in gene<br/>amplifications or rearrangements commonly seen in human tumours. I show that following geminin loss, cells complete an apparently …
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Diverse functions of LanC-like proteins in mammalian cells
… have been identified in various eukaryotic cells, but their function is not known. Intriguingly, the structure of the bi-functional enzyme CylM was solved and the dehydratase domain of LanM unexpectedly showed structural homology with mammalian kinases such as the mammalian target of …
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Delivery of biomolecules into mammalian cells using anthrax toxin
The intracellular delivery of biomolecules into mammalian cells is a major challenge due to the plasma membrane, which acts as a barrier between the extracellular environment and intracellular components. Recently, a non-toxic delivery platform derived from anthrax lethal toxin has been developed …
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Mathematical Modeling of Circadian Gene Expression in Mammalian Cells
… driven by the circadian gene expression in cells, which is regulated at both transcriptional and posttranscriptional stages. In this work, we first used mathematical modeling to investigate the transcriptional regulation of circadian gene expression, with a focus on the mechanisms of robust …
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