Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"malonyl-CoA"”.
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Protein and Pathway Engineering for Biosynthesis or Aromatic Compounds
… of phloroglucinol from three molecules of malonyl-CoA, and exhibited broad substrate specificity, which was successfully altered via saturation mutagenesis guided by a homology structural model of PhlD. To improve phloroglucinol production for industrial manufacturing, a two-prone approach …
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Characterization and analysis of genes encoding Escherichia coli acetyl-CoA carboxylase
Acetyl-CoA carboxylase catalyzes the first committed step of fatty acid biosynthesis, the synthesis of malonyl-CoA. In Escherichia coli the enzyme is a complex of four subunits that catalyzes two distinct half-reactions.
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Phosphorylation of Skeletal Muscle Acetyl-CoA Carboxylase by AMPK Enhances Palmitoyl-CoA Inhibition
Acetyl-CoA carboxylase (ACC) catalyzes the formation of malnoyl-CoA, which in turn controls the rate of fatty acid metabolism. ACC beta or 2 has been shown to be localized on the mitochondria in close proximity to carnintine palmitoyl transferase 1 (CPT-1), the enzyme responsible for the influx of …
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Autotrophe CO2-Fixierung in thermophilen Mikroorganismen
… reduktive Citrat-Zyklus, der reduktive Acetyl-CoA-Weg und der 3-Hydroxypropionat-Zyklus. Letzterer wurde bisher nur im phototrophen Bakterium Chloroflexus aurantiacus nachgewiesen. <br>In den letzten Jahren wurden zahlreiche thermophile und hyperthermophile Vertreter der Crenarchaeota isoliert, …
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Negative Regulation of the accBC Operon in Escherichia Coli
… Complementation of ACC activity using the malonyl-CoA synthase gene (matB) from Rhizobium trifolii allowed the deletion of the accA and accB genes. Transcriptional activity of the accBC operon in this strain shows an absence of growth-rate dependent regulation.
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Strain engineering and biosensor development for efficient biofuel production by Saccharomyces cerevisiae
… a genomic sensor that correlates intracellular malonyl-CoA concentration to a fluorescence signal by transcriptional regulation. Malonyl-CoA is the building block for the biosynthesis of fatty acids, 3-hydroxypropionic acid, polyketides, and flavonoids, which can either be used directly or be …
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The role of cysteine 230 and lysine 238 of biotin carboxylase in the deprotonation of biotin and synthesis of bisubstrate analogy inhibitor of carboxyltransferase
Acetyl-CoA carboxylase catalyzes the first step in the synthesis of fatty acids. The Escherichia coli form of the enzyme consists of a biotin carboxylase protein, a biotin carboxyl carrier protein, and a carboxyltransferase protein. This enzyme uses the cofactor biotin as a carboxyl carrier. In …
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Biosynthese, Akkumulation und Strukturen von Styrylpyronen in gametophytischen und sporophytischen Geweben von Equisetum
… Die KmWerte wurden für die Substrate Kaffeoyl-CoA und Malonyl-CoA bei 116 µM bzw. 141 µM ermittelt. Für die Substrate p-Cumaroyl-CoA und Malonyl-CoA lagen die KmWerte bei 182 µM bzw. 238 µM. Das relative Molekulargewicht des nativen Enzyms wurde mittels Gelfiltration mit 78-80 kD bestimmt. Im …
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Nuclear respiratory factor 1: a novel inhibitor of the human gene promoter of the cardiac isoform of acetyl-coenzyme a carboxylase
… Upstream, the cardiac-enriched isoform of acetyl-CoA carboxylase (ACCl3) synthesises malonyl-CoA, a potent inhibitor of CPT-1 and FA 13-oxidation. Since ACCl3 induction elevates malonyl-CoA levels, we hypothesised that AMPK activates NRF-1 thereby inhibiting ACCl3 expression in the heart to …
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Isolation and characterization of a synthetic gene and a genomic clone encoding acyl carrier protein from Escherichia coli
… enzymes, 3-ketoacyl-ACP synthase II and malonyl CoA-ACP transacylase, respectively. An open reading frame between acpP and fabD encodes a 26.5-kDa protein that has significant sequence identity ($>$40%) with a plant 3-ketoacyl-ACP reductase and thus is believed to encode the same enzyme …
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The impact of carbohydrate ingestion on the regulation of fat oxidation following exercise
… levels (p<0.05). The phosphorytataion of acety bCoA carboxylase (ACC), 5'AMP-activated protein klnase (AMPK) and hormone sensitive lipase (HSL) increased with exercise (p<0.05). ACC and AMPK phosphorylation in the CHO trial was lower at 1 h post than in the PLA trial (pc0.05). HSL phosphorylation …
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Characterization of a fatty acid elongase condensing enzyme by site-directed mutagenesis and biochemical analysis
… acyl group with a two carbon unit derived from malonyl-CoA. This study uses the condensing enzyme, EloA, from the cellular slime mold, Dictyostelium discoideum as a model for the family of ELOs. EloA has substrate specificity for monounsaturated and saturated C16 fatty acids and catalyzes the …
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The role of adiposity in the association of branched-chain amino acids and metabolic and clinical markers in healthy adults on controlled diets.
… opposing roles leptin and malonic acid-derived malonyl-CoA play in fatty acid metabolism. These exploratory metabolomic results provide key connections between metabolites related to BCAA catabolism, adiposity, inflammation, and adipokine activity. These results have broad applications towards …
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Investigating intermediates in 6-methylsalicylic acid biosynthesis
… in two steps. A non-hydrolysable carba(dethia)malonyl-N-acetylcysteamine analogue was synthesised and used to off-load enzyme-bound intermediates from 6-MSAS. In assays with acetyl-CoA and acetoacetyl-CoA alone, diketide and triketide intermediates were off-loaded and detected by …
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Molecular regulation of the cardiac-enriched acetyl-CoA carboxylase isoform (ACCβ) : a novel target for therapeutic interventions in cardiovascular disease
… 1 (CPT1). CPT1 is potently inhibited by malonyl-CoA, the product of the acetylCoA carboxylation reaction that is catalyzed by acetyl-CoA carboxylase (ACC). Recent studies have demonstrated that metabolic fuels such as fatty acids and glucose can function as signaling ligands, directing …
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Site and Mechanism of Leptin's Antisteatotic and Insulin Sensitizing Actions in Lipodystrophic Liver
… central leptin administration repressed stearoyl-CoA desaturase-1 (SCD-1) mRNA levels and enzymatic activity. We then studied SCD-1 s role in leptin-mediated correction of hepatic steatosis and insulin resistance by generating aP2-nSREBP-1c mice homozygous for SCD-1 deletion. These animals had …
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Investigation of molecular regulatory control of the cardiac-enrich acetyl-CoA carboxlase
… current study was centred on the enzyme acetyl-CoA carboxylase (ACC) which catalyses the carboxylation of acetylCoA to generate malonyl-CoA, a central metabolite in the regulation of fatty acid oxidation (FAO). There are two isoforms of ACC, ACCa. and ACCj3, both of which are present in the …
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Characterization of an operon containing a ribosomal protein gene and lipid biosynthetic genes in Escherichia coli K-12
… III. ORF6 was identified as fabD encoding malonyl-CoA/ACP transacylase. The fabG gene encoding 3- ketoacyl-ACP reductase and the acpP gene encoding acy] carrier protein are located just downstream of the fabD gene. Northern and promoter activity analysis demonstrated that the …
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Studies of Escherichia coli Acetyl-CoA carboxylase
Acetyl-CoA carboxylase (ACC) initiates the first committed step of fatty acid synthesis. ACC is composed of three separate components: biotin carboxylase (BC), carboxyltransferase (CT), and biotin carboxyl carrier protein (BCCP). BC carries out the first half of the ACC reaction where it catalyzes …
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Investigating mechanisms of triacylglycerol synthesis induced by nitrogen depletion in the diatom Phaeodactylum tricornutum and characterising its diacylglycerol acyltransferases and phospholipid: diacylglycerol acyl transferase
… and lipid metabolism in TAG accumulation: acetyl-CoA synthesis may increase while FA synthesis, the Kennedy pathway, malonyl-CoA/ACP transacylase, plastidial desaturase and FA transport enzymes were upregulated. Desaturases, elongases, Lands cycle enzymes and long chain acyl-CoA synthases are …
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