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Showing 1 to 20 of 20 for “"malic enzyme"”.
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Synthesis and degradation of rat liver malic enzyme and citrate cleavage enzyme
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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Increased expression of NADP malic enzyme in the guard cells of <i>Arabidopsis</i> plants
… of the transgene in these transformants. Enzyme assays shows higher ME activity in these transformants, indicating an active form of maize ME. Such transgenic plants would help to determine effects of increased ME activity on plant water loss.</p>
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A putative approach for influencing stomatal density by over-expressing NADP-dependent malic enzyme in arabidopsis
… density by over-expressing NADP-dependent malic enzyme (NADP-ME), which is thought to deplete the counter-anion pool in Arabidopsis thaliana guard cells. It was hypothesized that over-expressing NADP-ME in Arabidopsis will elevate stomatal density. Scanning electron microscopy was employed …
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Phyisiological, genomic and metabolic characterization of the oleaginous yeast Papiliotrema laurentii UFV-1
… such as the presence of a gene encoding for the malic enzyme NADP+-dependent and a single copy gene for the enzyme ATP citrate lyase, which is a key enzyme for oleaginous yeasts. We have found genes from alternative pathways for the lysine and leucine degradation, which may be alternative sources …
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The biochemistry of lipid accumulation in Mucor circinelloides and Mortierella alpina
… acid compositions were not affected. 4. Ten enzymes potentially linked to the regulation of lipid biosynthesis (fatty acid synthase, acetyl-CoA carboxylase, ATP:Citrate lyase, AMP:deaminase, carnitine acetyl transferase, malic enzyme, glucose-6- phosphate dehydrogenase, 6-phosphogluconate …
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Genetic engineering oleaginous yeasts for improved lipid production
… engineering routes. We found over-expressing malic enzyme and stearoyl CoA desaturase could improve lipid production 23% and 28 % in R. toruloides IFO0880 strain, but over-expression of glycerol-3-P dehydrogenase and pyruvate carboxylase failed to improve lipid titers. Deletion of peroxisomal …
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Effects of acute and chronic exercise on the activities of hepatic lipogenic enzymes in rats
… training on activities of five hepatic lipogenic enzymes were studied in rats fed different high-carbohydrate diets. In Study 1, ATP citrate lyase (CL), pyruvate kinase (PK), and fatty acid synthase (FAS) activities were greater in rats fed a high-fructose diet than in rats fed high-glucose or …
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Carbon dioxide fixation and glycogenesis in the flatworm Hymenolepis diminuta
… the in vivo incubation results. A low level of malic enzyme activity was also found in acetone powder extracts of the worm. It was too low in activity to be detected in spectrophotometric assays, but was studied through the measurement of C1402 incorporation into L-malate. Prom these preliminary …
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Fuels for flight in the fruit beetle,Pachnoda Sinuata, and control of flight metabolism
… lunata were found to have high activities of the enzymes alanine aminotransferase and NAD-dependent malic enzyme which are involved in proline metabolism, while the activities of these enzymes were lower in locust flight muscles. The activity of 3-hydroxyacyl-CoA dehydrogenase, an enzyme used in …
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Pyruvate Cycling Pathways and Glucose-Stimulated Insulin Secretion in Pancreatic Beta Cells
… pyruvate into the TCA cycle via the anaplerotic enzyme pyruvate carboxylase to engage one of several pathways of pyruvate recycling. Previous work demonstrated that pyruvate cycling was correlated with insulin secretion, and that NADPH may be involved in granule exocytosis. We hypothesized that …
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Population structure, biochemical adaptation and systematics in temperate marine fishes of the genera Arripis and Chrysophrys (Pisces: Perciformes)
… distinct snapper populations were detected. Enzyme kinetic studies were carried out on snapper isocitrate dehydrogenase (IDH) and salmon malic enzyme (ME) allozymes to determine whether or not functional differences existed in response to temperature fluctuation. It was found that differences …
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Population structure, biochemical adaptation and systematics in temperate marine fishes of the genera Arripis and Chrysophrys (Pisces: Perciformes)
… distinct snapper populations were detected. Enzyme kinetic studies were carried out on snapper isocitrate dehydrogenase (IDH) and salmon malic enzyme (ME) allozymes to determine whether or not functional differences existed in response to temperature fluctuation. It was found that differences …
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Metabolic flux analysis of Escherichia coli MG1655 under octanoic acid (C8) stress
… under stress: the TCA cycle flux by ~ 44%; the malic enzyme pathway by ~ 80%; the phosphoenolpyruvate carboxylase pathway ~ 60%; the CO2 production rate by 18%; and pyruvate dehydrogenase pathway by ~50%. Meanwhile, a few pathways were activated under C8 stress: the pyruvate dehydrogenase flux …
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Metabolic pathways of Campylobacter fetus
… glucose and citrate; acetate had no effect. All enzyme assays were performed at 25 C in a Perkin-Elmer model 124 double beam spectrophotometer. The reaction mixture was 3 ml which contained the appropriate substrate, cofactors and properly diluted crude cellular extract. The specific activity of …
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Characterization of coconut oil and CLA induced lipolysis
… Sterol regulatory binding protein 1c and malic enzyme expression was least in CO+CLA-fed mice. Study 2: 3T3-L1 cells were differentiated, exposed to CO or SO for 10d, serum starved overnight, and pre-loaded with 3[H]-oleic acid for 12 hrs. Cells were treated with 50 muM CLA or linoleic …
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THE ROLE OF NRF2 IN PREVENTING OXIDATIVE/ELECTROPHILIC STRESS-INDUCED LIVER INJURY
… involved in the pentose phosphate pathway and malic enzyme levels had a clear dose-dependent correlation to active levels of Nrf2, indicating that Nrf2 promotes NADPH generation. Among genes suppressed by Nrf2, the majority are involved in lipid synthesis and fatty-acid desaturation. Thus, Nrf2 …
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Carbon-13 metabolic flux analysis of soybean central carbon metabolism: response to temperature and genotype effects
… anaplerotic reactions catalyzed by the plastidic malic enzyme (mep), cytosolic pep carboxylase (ppc) and the malate transporters (malT1 and malT2) between cytosol, mitochondrion and plastid varied considerably with temperature. The redirection of carbon between these cellular compartments had a …
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Metabolic engineering and modelling of Escherichia coli for the production of succinate
… can be seen as one of the most crucial enzymes for succinate production. This enzyme converts succinate into fumarate and therefore has to be knocked out to increase production. Strains that possess a succinate dehydrogenase deletion immediately show an increased production. However, …
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Discovery of a Novel Signaling Circuit Coordinating Drosophila Metabolic Status and Apoptosis
<p>Apoptosis is a conserved mode of cell death executed by a group of proteases named caspases, which collectively ensure tissue homeostasis in multicellular organisms by triggering a program of cellular "suicide" in response to developmental cues or cellular damage. </p><p>Accumulating evidence …
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Understanding the physiological and molecular basis of chilling tolerance across species of the C4 genera Miscanthus and Spartina
… (PPDK) or one of the three decarboxylating enzymes specific to the C4 sub-type, or 2) the fact that C4 photosynthesis evolved relatively recently from C3 species of tropical and sub-tropical origin, suggesting that there has not been sufficient evolutionary time for species possessing C4 …