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Showing 1 to 20 of 92 for “"malate"”.
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A characterization of chicken heart mitochondrial malate dehydrogenase
Chicken heart mitochondrial malate dehydrogenase has been purified by an improved isolation method to give 56% of the initial mitochondrial enzyme. The purified enzyme has a specific activity of 340 U/mg. This homogeneous enzyme has been shown to be pure by several criteria. The enzyme has been …
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Purification and characterization of malate dehydrogenase and 6- phosphogluconate dehydrogenase from Haemophilus influenzae
… effect on growth of the organism. Haemophilus malate dehydrogenase was purified 109-fold with a 26% recovery through a 4-step procedure involving salt fractionation, and hydrophobic and dye affinity chromatography. The purified enzyme was demonstrated to be a dimer of M,= 61,000. Initial …
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Role of Malate in the Regulation of Nitrate Uptake and Assimilation in Corn (Zea Mays L.) Seedlings
Made available in DSpace on 2015-05-14T14:57:42Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 7511544.PDF: 3357556 bytes, checksum: 900e081d0c241e13314336021b48b101 (MD5) Previous issue date: 1974
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The oxidative metabolism of malate plus pyruvate, and a-ketoglutarate by vitamin E-deficient rat liver mitochondria
… substrate conditions: endogenous, pyruvate plus malate, and α-ketoglutarate, were measured by oxygen electrode analysis. Statistically significant differences were seen among state 4 respiration with α-ketoglutarate, state 4 respiration(combined data with endogenous, pyruvate plus malate and …
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Myoglobin redox form stabilization: role of metabolic intermediates and NIR detection
… metmyoglobin (MMb) reduction and oxidation of malate to α-ketoglutarate with regeneration of reduced nicotinamide adenine dinucleotide (NADH) via malate dehydrogenase (MDH). Our specific objectives for this experiment were: (1) to examine the interaction of malate and MDH to reduce MMb in …
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Lithium-based inorganic-organic framework materials
… using substituted forms of the ligand: lithium L-malate, Li2(C4H4O5), lithium methylsuccinate, Li2(C5H6O4), and lithium tetrafluorosuccinate, Li2(C4F4O4). The cell parameters and mechanical properties of these non-porous frameworks were found to depend on inter-ligand interactions and metal-ligand …
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Mathematical modelling of mammalian mitochondrial metabolism
… (HRTTCA) showed that it needs an input from the malate-aspartate shuttle reactions and therefore a mammalian heart-specific malate-aspartate shuttle model was built. Analysis of the malate-aspartate shuttle model revealed that in order to supply the heart TCA cycle with anaplerotic input, the …
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Identification And Characterization Of The Major Gene Ma And Its Associated Co-Expression Gene Networks Regulating Apple Fruit Acidity
… taste and flavor is primarily determined by malate concentrations. Previous studies reported that apple fruit acidity was predominantly controlled by the major QTL Ma. To better understand apple fruit acidity, this study attempts to identify and characterize the gene underpinning Ma and its …
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Carbon dioxide fixation and glycogenesis in the flatworm Hymenolepis diminuta
… the measurement of C1402 incorporation into L-malate. Prom these preliminary studies the enzyme did not appear abundant enough to be physiologically significant. A third CO2 fixation activity was found in the acetone powder extracts. It appeared to be different from any enzyme yet found and …
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Integration of composite nanomaterials into anode and cathode designs
… materials for the oxidation of NADH and L-Malate oxidation by poly(methylengreen) and malate dehydrogenase respectively. SWNTs were used to create an electrode design consisting of high surface area-hierarchically ordered carbon nanomaterials with controlled surface chemistry. Additionally, …
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Increased expression of NADP malic enzyme in the guard cells of <i>Arabidopsis</i> plants
… from neighboring cells into guard cells, and malate synthesis within guard cells increases turgor pressure opening the stomata. Stomata close when K<sup>+</sup> and Cl- efflux out, and the cytosolic isoform of NADP ME convert malate to pyruvate. We hypothesize that stomatal closure can be …
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The effect of beta-oxidation or TCA cycle inhibition on mitochondrial function and the sensitivity of high resolution respiratory detection
… cycle alone, and b) Octanoylcarnitine and malate, which assesses the influence of the TCA cycle. Additionally, a combination of pyruvate, glutamate and malate is used to assess oxidation within the TCA cycle. We investigated the sensitivity of commonly used substrate combinations in HRR …
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Functional Genomics of Nitrogen Use in Maize
… to funnel C toward asparagine and away from malate, a C4 carrier and TCA intermediate. B73 showed a greater preference for malate synthesis at the transcript abundance and metabolite levels, although both lines responded to N with greater chlorophyll and amino-N, increased expression of amino …
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The glyoxylate shunt as a target for antibacterial intervention in Pseudomonas aeruginosa
… comprised of two enzymes: isocitrate lyase and malate synthase G, and allows the synthesis of cellular constituents from C2 nutrient sources, bypassing the decarboxylation steps of the citric acid cycle when glucose is not available. The glyoxylate shunt has potential as a new antibacterial …
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The role of GABA in plant salinity and hypoxia responses
… pH regulates the activity of Aluminum-activated Malate Transporter proteins (ALMTs), which are proteins shown to catalyse the movement of carboxylate ions across membranes and are inhibited by GABA. Specifically, the impact of a histidine residue within the putative GABA binding domain of wheat …
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