Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"major facilitator superfamily"”.
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Stressed Out: Resistance-nodulation-division (RND) and Major Facilitator Superfamily (MFS) Efflux Pump Mediated Survival Strategies of Acinetobacter baumannii
Originally discovered in soil microbial communities, reports of genus Acinetobacter associated infections started to increase around 1977 and continued to climb to frightening heights for the next 20 years resulting in Acinetobacter becoming a high priority target for antibiotic resistance …
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MFSD7C is an ATP Transporter that Supports Bacterial Killing by Alveolar Macrophages in a Lipid-Rich Microenvironment
Major Facilitator Superfamily Domain-Containing 7C (MFSD7C) is a solute carrier with unknown substrate. In humans, mutations in MFSD7C cause Fowler syndrome, which is characterized by aberrant development of fetal vasculature in the brain resulting in hydranencephaly-hydrocephaly. Although most …
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MFSD7C: A Solute Carrier Linking Heme and Calcium in Mitochondrial Energy Metabolism
Major Facilitator Superfamily Domain-Containing 7C (MFSD7C) is an orphan solute carrier. In humans, loss-of-function mutations in MFSD7C cause Fowler syndrome, an often prenatally lethal disorder that affects brain development and angiogenesis. MFSD7C and its closely related paralog MFSD7B, have …
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Purification and characterisation of the Escherichia coli multidrug efflux protein MdtM
… amino acid residues protein from the ubiquitous major facilitator superfamily (MFS). This thesis focuses on the purification and characterisation of MdtM. Functional characterisation of MdtM using growth inhibition assays and whole cell transport assays revealed its role in intrinsic resistance …
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Development of pseudosymmetry analysis to identify key residues in transport protein mechanisms
… Parameters for GAPS-Pro are developed for three major superfamilies of transporter: the mitochondrial carrier family, the major facilitator superfamily, and the amino acid polyamine organocation superfamily. The software is then tested on transporters for which there is a large amount of …
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Mechanistic studies of a multidrug efflux protein from <i>Escherichia coli</i>
… coli is MdtM, a 12 TM antiporter of the Major Facilitator Superfamily (MFS). MdtM, like other members of the MFS, utilises the energy stored in the electrochemical gradient to drive the extrusion of drugs from the cytoplasm. Therefore, protons take part in many critical interactions with …
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Characterization and engineering of sugar transporters in the yeast Kluyveromyces marxianus for biotechnological applications
… 12 genes that encoded transporters of the major facilitator superfamily (MFS). Using this ΔPT platform, which was unable to grow on pentoses, we identified several native K. marxianus transporters that recovered the native strain’s ability to grow on either xylose and/or arabinose. Kinetic …
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Computational methods to design biophysical experiments for the study of protein dynamics
… transporter a typical representative of the Major Facilitator Superfamily of membrane proteins. This was followed by ideal double electron-electron resonance experimental choice predictions based on the simulation data. The predicted choices are superior to the residue-pair choices made by …
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Structural and Mechanistic Studies of Multidrug Resistance Efflux Pumps in Multiple Families
… evidence on this ion’s identity. The Major Facilitator Superfamily (MFS) is the largest family of drug transporters, but until recently none of its members have been structurally elucidated in both inward- and outward- facing states. These are now available for MdfA, and we have …
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Mechanism and energetics of membrane transporters and channels
… cell in exchange for inorganic phosphate (Pi ). Major facilitator superfamily (MFS) is the largest superfamily of secondary active transporters and catalyze the transport of an enormous variety of small solute molecules across biological membranes. Individual MFS members, despite their …
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Analysis of the diverse antibacterial strategies used by <i>Serratia marcescens</i> Db10
… in <i>S. marcescens</i> Db10. The role of the Major Facilitator Superfamily protein, Alb1, in the export of and resistance to althiomycin is also defined. Using a combination of bioinformatic, biochemical and genetic approaches we demonstrate that a PPTase enzyme, PswP, is essential for …
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Mechanistic insights into the secondary-active multidrug transporter LmrP
… by bacterial multidrug transporters. LmrP is a Major Facilitator Superfamily (MFS) multidrug transporter which, when overexpressed in bacteria, can confer resistance to 22 clinically important antibiotics. The structural data for MFS proteins support an alternating access mechanism, in which …
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Physical Activity-Associated Differences in Placental Lipid Profiles and Docosahexaenoic Acid Uptake: Insights from Lipidomics and an In Vitro Exercise Model
… were objectively measured using accelerometry. Major facilitator superfamily domain containing 2a (MFSD2a) protein expression in placental tissue was measured semi-quantitatively using Western blotting. The second objective was to evaluate DHA uptake in vitro. Primary trophoblast cells were …
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Characterization of activation tagged potato (Solanum tuberosum L.) mutants
… encoding an Acyl-CoA N-acyltransferases (NAT) superfamily protein, and a predicted major facilitator superfamily protein (MFS) were identified and overexpression lines Bintje/35S::NAT1 and Bintje/35S::PMT1 were created for recapitulation of the nikku mutant phenotype. Methylated DNA-PCR between …
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Investigation of Clinically Relevant Fluconazole Resistance Mechanisms in the Fungal Pathogen Candida parapsilosis
… of resistance, presents investigations of three major mediators of fluconazole resistance, and characterizes a collection of clinical isolates to better identify and understand how fluconazole resistance in C. parapsilosis. A recently developed CRISPR-Cas9 system was used to edit CpMRR1 alleles …
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Transcriptional Regulation of Azole Antifungal Resistance in Candida albicans
… the development of azole resistance.</p> <p>Two major mechanisms of azole resistance exist in C. albicans, the up-regulation of genes encoding efflux pumps and the up-regulation of ERG11, a gene encoding the azole drug target lanosterol demethylase. The overexpression of efflux pumps remain the …