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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"major ampullate"”.
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Characterization of a family of cysteine rich proteins and development of a MaSp1 derived miniature fibroin
… are present within the spinning dope of the major ampullate gland using MS analysis. Biochemical studies and characterization of one of the family members, CRP1, demonstrate that this factor can self-polymerize into higher molecular weight complexes under oxidizing conditions, but can be …
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Characterizing small molecular weight proteins from Latrodectus hesperus dragline and tubuliform silks
… RT-PCR analysis from mRNAs collected from the major ampullate gland that transcript levels for CRP-family members from non-silked and a silked spider are different. CRP2 and CRP4 mRNA levels were shown to increase upon silking. Overall, the major findings of this thesis involved characterizing …
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Molecular identification of silk proteins in the gumfooted lines and attachment discs of the black widow spider, latrodectus hesperus
… lines were identified to be members of the Major Ampullate Spidroin family (MaSp), using mass spectrometry. This research was the first to identify the core fibroins of the gumfooted lines.</p> <p>Novel peptide fragments from solubilized gumfooted lines were acquired from manual de novo …
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Characterization of the large diameter fibers in egg case silk : identification of a core fibroin, TuSp1, and localization of fibroin-like molecules, ECPs, from the black widow spider, latrodectus hesperus
… sub-repeats, which are commonly found in minor ampullate and major ampullate silks. Polyserine blocks and short polyalanine stretches were highly represented in the TuSp 1 amino acid sequence. Our data support the assertion that TuSp 1 represents the main constituent within egg case silk. This …
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Part I characterization of MyoR in C2C12 mouse fibroblasts. Part II isolation and characterization of a novel class II bHLH transcription factor from the black widow spider, latrodectus hesperus
… glands, which includes the tubuliform and major ampullate glands. BW6 is capable of binding an E-box element as a heterodimer with E2A, but was unable to bind this motif as a homodimer. BW6 is also capable of inhibiting the transactivation of rE47 in mammalian cells. BW6 represents the …
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Spinning of synthetic spider silk-like fibers and ex vivo rheology of spider silk
… (-1 microliter) available from the spider's major ampullate gland. A shear thinning behavior of the dope was observed using the microrheometer. The capillary break-up rheometer was used for extensional rheology. The rheological findings indicate potential mechanisms that could be employed by …
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Funktion und Einfluss der nicht-repetitiven, terminalen Domänen auf Speicherung und Assemblierung von Spinnenseidenproteinen
… aufgebaut, die als MaSp1 und MaSp2(Major Ampullate Spidroin) bezeichnet werden. Die mehrere hundert Kilodalton großen Spidroine bestehen im Wesentlichen aus einer repetitiven, strukturell ungeordneten Kerndomäne, die von relativ kleinen, ca. 100-140 Aminosäuren langen …
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Development of a codon-optimized Latrodectus hesperus MaSp1 synthetic gene for bacterial protein expression using a seamless cloning strategy
<p>Spider silk has outstanding mechanical properties, displaying high tensile strength and extensibility. The unique combination of strength and great extensibility make it one of the toughest materials in the world. Of the seven different spider silks, dragline silk, the lifeline silk of the …
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Influencia de la radiación ultravioleta en el comportamiento mecánico y en la microestructura de las fibras de seda de araña
… for this work was silk fibers spun from the major ampullate silk gland (MAS) of the species Argiope trifasciata. The tensile properties of silk fibers subjected to different irradiation times with UV light of wavelength 254, 312 and 365 nm close -to bond energies of the polymeric backbone- …