Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"magnetic twisting cytometry"”.
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E-cadherin mechanotransduction beyond cell-cell junctions
… in mechanotransduction, using modified Magnetic Twisting Cytometry (MTC) and Traction Force Microscopy (TFM) coupled with fluorescence imaging. A major focus of Chapter 2 is the role of a cadherin-associated protein α-catenin in force transduction. MTC and TFM studies using α-catenin …
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Rapid Rac GTP-ASE activation in live cells by mechanical stress is independent of Src
… at the cellular and molecular level. Using the magnetic twisting cytometry technique, we applied local mechanical stresses to living human airway smooth muscle cells with a magnetic bead bound to the cell surface via transmembrane adhesion molecule integrins. The temporal and spatial activation …
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Mechanical regulation of cell-cell junctions
… adhesion and mechanotransduction, using bead-twisting measurements in conjunction with confocal fluorescence imaging. These experiments revealed rapid, early molecular events in mechanotransduction and the rudiments of a mechanotransduction mechanism. I further investigated how internal …
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Biophysical and gene expression change in living cells by force-induced mechanotransduction
Within the past decade, there has been abounding scientific evidences supporting the notion that mechanical forces are crucial in regulating the physiologic functions of cells and tissues. The importance of engineering principles in studying the biological behavior of cells is no longer in …
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Cyclic loading of fibronectin coated beads induces differentiation in mouse embryonic stem cells
… directs mouse embryonic stem cell (mESC) fate. A magnetic twisting cytometer (MTC) was used to apply oscillating force to mES cells through fibronectin (FN) coated magnetic microbeads. The ligand coated magnetic beads were allowed to bind to undifferentiated mES cells plated on 0.6 kPa …