Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 99 for “"magic-angle-spinning"”.
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Carbon-13 magic angle spinning NMR studies of myelin membranes
… T), high-resolution $\sp{13}$C solid state ""magic angle"" sample spinning nuclear magnetic resonance (MAS NMR) spectra of a variety of model phospholipids, sphingolipids, myelin and white matter samples, resolving and assigning over 60 resonances in the spectra of human myelin samples. …
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Advances in Instrumentation for Dynamic Nuclear Polarization & Magic-Angle Spinning NMR
… tool for increasing the sensitivity of magic angle spinning (MAS) nuclear magnetic resonance (NMR) experiments. Historically, one of the primary drawbacks of DNP has been limited spectral resolution. The resolution of DNP spectra can be substantially improved via data acquisition at …
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Assessment of magic angle spinning spectroscopy for studying migration in solid milk chocolate
In the confectionery industry, there is considerable interest in understanding the mechanisms of liquid lipid migration inside chocolates as it relates to combating fat-bloom defects in confectionery products. [1] This thesis investigates the ability of MAS NMR spectroscopy to adequately resolve …
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Structural Characterization of Plaque Seeded Amyloid-β Fibrils by Magic Angle Spinning NMR
Accumulation of plaques consisting of amyloid fibrils of the peptide amyloid-β (Aβ) in the brain is one of the hallmarks of Alzheimer’s disease (AD). Aβ is prone to polymorphism and the structure of fibrils is sensitive to the conditions under which they are formed. The two dominant forms of Aβ are …
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Structure determination of proteins and protein aggregates by magic-angle spinning solid-state NMR
Solid state NMR (SSNMR) is a structure determination technique uniquely suited to study protein aggregates and fibrils. Unlike solution NMR or X-ray crystallography, SSNMR can obtain atomic resolution structural information on samples of protein fibrils which are insoluble and do not produce X-ray …
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Monitoring apoptosis in intact cells by high-resolution magic angle spinning 1H NMR spectroscopy
… investigates the application of High-Resolution Magic Angle Spinning (HRMAS) 1H NMR spectroscopy in monitoring metabolic changes in intact cells during apoptosis. The technique is used to analyse metabolic profiles of cells treated with cisplatin and etoposide after 3, 8, 24 and 48 h. The results …
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Elucidating the pumping mechanism of bacteriorhodopsin using dynamic nuclear polarization enhanced magic angle spinning NMR
Bacteriorhodopsin (bR) is comprised of 7 trans-membrane helices that enclose a retinylidene chromophore formed by a Schiff base (SB) between the retinal and Lys216. Due to bR's relative availability, it serves as a model for other members of the rhodopsin family, ion channels and GPCRs. Since its …
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Studies of Structure, Dynamics, and Interactions of Amyloid Fibrils by Magic Angle Spinning Solid State NMR
… such as X-ray crystallography or solution NMR. Magic angle spinning (MAS) solid state NMR is a perfectly suitable method to study the amyloid fibrils, and is able to cover a very broad range of topics, from structures, dynamics to interactions. The theme of this thesis focuses on various topics …
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Magic angle spinning NMR applied to membrane protein 2D crystals : the structure and function of VDAC
… In pursuit of this objective we have applied Magic Angle Spinning Nuclear Magnetic Resonance (MAS NMR) to investigate the structure and function of the 32 kDa human integral membrane protein VDAC, the Voltage Dependent Anion Channel. Our results on VDAC have relied on a number of improvements …
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Amyloid fibril structure of peptides and proteins by magic angle spinning NMR spectroscopy and dynamic nuclear polarization
… to form amyloid fibrils in vitro. We have used magic angle spinning (MAS) NMR spectroscopy to investigate the structure of two amyloid fibril systems - an 11- residue segment from the disease-related protein transthyretin (TTR); and P2- microglobulin (32m), a 99-residue protein associated with …
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Structural Studies of Amyloid-β Fibrils using Magic Angle Spinning Nuclear Magnetic Resonance and Dynamic Nuclear Polarization
Magic Angle Spinning Nuclear Magnetic Resonance (MAS NMR) is a powerful method that probes the structure and dynamics of insoluble molecules at atomic level detail. The last two decades has seen a rise in studies of amyloid fibrils, which have been implicated in many diseases. In particular, …
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Solid-State Magic-Angle Spinning NMR Methods for Tensor Measurements and Protein Structure Refinement Using Chemical Shift Tensors
… (CSA) pseudostatic powder lineshapes, slow magic angle spinning (MAS) analysis of highly-13C,15N-enriched solid proteins using 2D heteronuclear correlation at 750 MHz 1H frequencies, rotational resonance (R2) lineshape analysis, and distance information using the REDOR and TEDOR …
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Structure and function of the Influenza membrane protein M2 by magic angle spinning NMR and dynamic nuclear polarization
… structural characterization of proteins using magic angle spinning (MAS) nuclear magnetic resonance (NMR) applied to the membrane protein M2 from Influenza A, a small helical transmembrane proton transporter that is the target of adamantane based inhibitors but which is now drug resistant due …
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Investigation of structural phase transitions in minerals and analogue systems by high-temperature magic-angle-spinning nuclear magnetic resonance spectroscopy
… magnetic resonance spectroscopy using mostly magic-angle spinning (MAS) techniques. The results provide important insight into each of these SPT's and illustrate the types of information that can be obtained about SPT's using MAS NMR spectroscopy.
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Biomarkers of cell stress and cell death detected by proton high resolution magic angle spinning (\(^1\)H HR-MAS) nuclear magnetic resonance (NMR) spectroscopy in a rat glioma cell line
… study is to determine \(^1\)H high-resolution magic angle spinning (HR-MAS) nuclear magnetic resonance (NMR) biomarkers of cytostasis and cell death in a rat glioma BT4C cell line. Cytostasis and cell death were induced in BT4C cells using cisplatin and substrate free medium, respectively. Cell …
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Solid state nuclear magnetic resonance methodology for biomolecular structure determination
… are presented. All studies are performed with magic angle spinning (MAS) and high-power proton decoupling, for optimal sensitivity and resolution. Chemical shift are assigned by multi-dimensional correlation spectroscopy in isotopically enriched molecules ...
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Multidimensional Solid-State NMR Studies of Membrane Proteins
… optimized the performance of shaped pulses in magic-angle spinning solid-state NMR experiments by characterizing their frequency profiles and refocusing efficiency under conditions commonly used for protein spectroscopy. We have also identified the correct rotor-synchronization condition, which …
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Synthesis of Selenium-Alkylated Selenocysteine Derivatives and Their Use in Native Chemical Ligation, Expressed Protein Ligation and the Synthesis of Dehydropeptides
… These solid phase reactions were monitored by magic angle spinning NMR spectroscopy.
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Multinuclear Solid-State NMR Studies of Supported Transition Metal Carbonyls
… and oxide-supported metal carbonyl complexes. Magic-angle spinning (MAS) C-13 NMR spectra of polycrystalline metal carbonyl clusters yield both isotropic chemical shifts and chemical shift anisotropies (CSA's) for the carbonyl ligands, providing information on solid-state symmetry, J-coupling, …
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Deformation induced molecular behavior of Cis 1,4-polyisoprene and its nanocomposites monitored by solid-state NMR
… strain. These are the first experiments to use magic angle spinning nuclear magnetic resonance techniques in situ with compression measurements to identify changes in the chain environment during compressive strain. For the polyisoprene composites, in situ compression measurements were made …
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