Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"macromolecular crowding"”.
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The effect of macromolecular crowding on the structure of the protein complex superoxide dismutase
… <em>in vitro.</em> This effect is referred to as macromolecular crowding and constitutes an important physical difference between native biological environments and the simple solutions in which biomolecules are usually studied.^ We used small angle scattering (SAS) to measure the effects of …
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From macromolecular crowding to transcriptional regulation: Exploring the complexities of cytochrome P450s, cannabinoids, and inflammation
… 2 is the investigation of lipid composition and macromolecular crowding and their effects on the stability and protein-protein interactions of the Cytochrome P450 2J2 (CYP2J2) and its redox partner Cytochrome P450 Reductase (CPR). CYP2J2 is a membrane-bound epoxygenase expressed in cardiac tissue …
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Macromolecular crowding effects on the excited-state dynamics of novel FRET probes using time-resolved fluorescence
… still a need to develop a technique to quantify crowding concentrations in live cell studies. Recently, several fluorescence-based probes have been engineered to potentially quantify crowding within living cells. These dynamic probes contain a pair of fluorophores (mCerulean3 and mCitrine, the …
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Effects of macromolecular crowding and small ions on the folding, structure, and stability of Desulfovibrio desufuricans flavodoxin
… in vitro studies. In addition to intracellular macromolecular crowding, cells are ionic in nature, and although the Hofmeister series of ions has its origin in a work from 1888, much is still unclear concerning how small, charged ions affect protein properties. This thesis summarizes in vitro …
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The Influence of Osmolytes on Nucleic Acid Helical Conformations
… cell, nearly 40% of the volume is occupied by macromolecular crowding agents and smaller osmolytes that accumulate in response to environmental stresses. The effects of these cosolutes were observed on the transition of helical conformation from B-DNA to Z-DNA. Distinct from the familiar, …
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Applications of fluorescence detected sedimentation I Studying the thermodynamic impact of valence in non-ideal solutions II High affinity interactions and biological media
… conditions will bridge what we know about macromolecular diffusion and assembly under ideal conditions and what has been learned about diffusion and assembly in the context of intact cells. New findings in this area will help to build upon what is already known about macromolecular crowding …
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Daily organisation of cellular physiology by mTOR kinase
… in mTORC activity e.g., protein synthesis and macromolecular crowding. In my research I have sought to gain insight into the nature of the interaction between circadian regulation and mTORC signalling, in vitro and in vivo. My lab previously observed reciprocal circadian rhythms in the …
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Single-Molecule and Ensemble Measurements Inform on the Role of Protein Crowding at Interfaces
… aim of this dissertation was to characterize the crowding mechanism of the most abundant serum protein, serum albumin. Many studies have explored serum protein conformation on albumin-crowded surfaces, but unanswered questions about the mechanism of interaction between different proteins remain. …
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Temperature- and pressure-induced protein dynamics from microseconds to minutes
… of phosphoglycerate kinase is influenced by macromolecular crowding. In Chapter 7, an outreach project is described where simple mechanical and computer models of protein folding were used to teach students at the high school and undergraduate levels about scientific modeling and other basic …
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Dynamics of E. coli genome and cytosol under antibiotics
… state of locus ”compaction” and the levels of macromolecular crowding of the cytosol, respectively. We improve the precision of those measurements developing a novel data treatment procedure and discover that sub-lethal doses of ciprofloxacin, rifampicin, and vancomycin as well as hyperosmotic …
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Single-Molecule Studies Of Local And Global Nucleic-Acid Dynamics
… RNA global motions in conditions with molecular crowding, which mimics the cellular environment, are reported using the cyclic-diguanylate monophosphate (c-di-GMP) riboswitch aptamer domain as a model. Riboswitches are examples of gene-regulating elements that normally act in cis to their host …
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Cell-Free Biosystems Comprised of Synthetic Enzymatic Pathways: Development of Building Blocks, Immobilization of Enzymes, Stabilization of Cascade Enzymes, and Generation of Hydrogen
… enzyme (e.g., PGI) due to the in vitro macromolecular crowding effect. And substrate channeling among CBM-tagged self-assembled three-enzyme complex (synthetic matabolon) immobilized on the easily-recycled cellulose-containing magnetic nanoparticles can not only increase cascade reaction …
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Insights into the structure and aggregation of lens crystallins and other aggregation-prone proteins
… of the molecular chaperone alphaB-crystallin. Macromolecular crowding using Ficoll 400 induces significant destabilisation, unfolding, an increase in size/oligomeric state, and a loss of chaperone function leading to kinetically distinct amorphous and fibrillar aggregation. These results are …
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Insights into the structure and aggregation of lens crystallins and other aggregation-prone proteins
… of the molecular chaperone alphaB-crystallin. Macromolecular crowding using Ficoll 400 induces significant destabilisation, unfolding, an increase in size/oligomeric state, and a loss of chaperone function leading to kinetically distinct amorphous and fibrillar aggregation. These results are …
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Investigating the self-assembly and molecular chaperone ability of casein proteins and polyproline
… lactose, lipids, and heparan sulfate) and crowding agents were investigated on reduced and carboxymethylated (RCM) kappa-casein fibril formation. Longer-chain phosphatidylcholine lipids enhanced RCM kappa-casein fibril formation, whereas shorter chain phospholipids and triglycerides had …
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Investigating the self-assembly and molecular chaperone ability of casein proteins and polyproline
… lactose, lipids, and heparan sulfate) and crowding agents were investigated on reduced and carboxymethylated (RCM) kappa-casein fibril formation. Longer-chain phosphatidylcholine lipids enhanced RCM kappa-casein fibril formation, whereas shorter chain phospholipids and triglycerides had …
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Characterizing and Influencing Intracellular Transport
… Paired with this analysis, we also observed that macromolecular crowding has no effect on directed transport through the reduction of ribosome concentration, indicating that directed intracellular transport is quite efficient despite significant obstacles in the cytosol. Looking further at the …
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