Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 42 for “"macromolecular complexes"”.
-
Coarse-Grained Molecular Dynamics Simulations of Macromolecular Complexes
… molecular dynamics simulations of large macromolecular complexes on relevant time scales, using a simplified, or ""coarse-grained"" description. The method developed for this purpose, called shape-based coarse-graining, is applied to a number of systems. Simulations of several viruses, …
-
Integration of Structural Methods to Characterize the Dynamics of Macromolecular Complexes
… the structure, and function, of various macromolecular complexes beyond the conventional low-energy, singular state approach. To do this, we extend existing methods and create new approaches catered for the study of large-scale macromolecular dynamics. Here, we characterize the …
-
Cobalt Nanoparticle-Macromolecular Complexes and Their Conversion to Oxidatively Stable Entities
… in this dissertation was to synthesize novel macromolecular materials that would afford oxidative stability to magnetic cobalt nanoparticles under ambient conditions. The cobalt nanoparticles were formed via the thermolysis of Co2(CO)8 in concentrated solutions of toluene containing the …
-
From Homogeneous To Heterogeneous: Statistical 3-D Signal Reconstruction Of Macromolecular Complexes
The structure and function of biological macromolecular complexes is currently a topic of great interest in biology. The primary contribution of this thesis is the mathematical description of a specific problem in this area of biology, the development of algorithms and software to solve the …
-
Assembly And Function Of Macromolecular Complexes For Accurate Trna Aminoacylation In Helicobacter Pylori
… </p> <p> <strong>ASSEMBLY AND FUNCTION OF MACROMOLECULAR COMPLEXES FOR ACCURATE TRNA AMINOACYLATION IN <i>HELICOBACTER PYLORI</i> </strong> </p> <p>by</p> <p> <strong>GAYATHRI SILVA</strong> </p> <p>January 2014</p> <p>Advisor: Dr. Tamara L. Hendrickson</p> <p>Major: Chemistry …
-
Macromolecular Complexes of Cystic Fibrosis Transmembrane Conductance Regulator Alter Fluid Transport in inflammatory Bowel Disorders
<p>Macromolecular complexes of cystic fibrosis transmembrane conductance regulator (CFTR) comprise of network of proteins that can regulate cAMP-/cGMP-activated CFTR chloride channel function. We report the physical and functional coupling of CFTR with nitric oxide (NO) producing enzyme-inducible …
-
Physical and Functional Coupling of CFTR and PDE3A
<p>Formation of multiple-protein macromolecular complexes at specialized subcellular microdomains increases the specificity and efficiency of signaling in cells. In this study, we demonstrated that phosphodiesterase type 3A (PDE3A) is physically and functionally coupled to cystic fibrosis …
-
Design framework of the MuA remodeling signal that confers preferential complex disassembly by the AAA+ unfoldase ClpX
… of ATPases are unfolding chaperones that remodel macromolecular complexes and facilitate ATP-dependent protein degradation. They are members of the superfamily of AAA+ enzymes (ATPases Associated with various cellular Activities), which is conserved across all kingdoms of life. Efficient selection …
-
Hormonal regulation of phosphodiesterase 3B in adipocytes
… stimulate the recruitment of PDE3B into large macromolecular complexes in adipocytes. The recruitment is dependent on caveolin-1 especially in response to insulin and the constituents of the PDE3B macromolecular complexes are dependent on the stimuli used. After insulin stimulation insulin …
-
Obscurin Mediates Ankyrin Complex Formation In The Heart
… And while some of these obscurin-ankyrin complexes have been studied, many others have not been characterized. Further, previous studies have focused on these ankyrin-obscurin complexes individually; however, how ankyrin-mediated macromolecular complexes are integrated together within the …
-
Cryo-electron microscopy image analysis using multi-frequency vector diffusion maps
… technique for 3D structure determination of macromolecular complexes. However, because the images are taken at low electron dose, it is extremely hard to visualize the individual particle with low contrast and high noise level. In this thesis, we propose a novel approach called …
-
Molecular Biophysics of Mammalian and Fungal Macromolecular Assemblies
… allows for the collection of data reflecting the macromolecular organization of an ordered structure, and even intramolecular interactions of nuclei. This thesis details two projects that utilized NMR to gain a higher understanding of two macromolecular assemblies; each project has been divided …
-
Segmentation and registration of molecular components in 3-dimensional density maps from cryo-electron microscopy
… is a method that produces 3D density maps of macromolecular complexes. Segmentation and registration methods are heavily used to extract structural information from such density maps. Segmentation aims to identify regions in a density map corresponding to individual molecular components, so as …
-
Mechanical manipulation and visualization of single biomolecules using fluorescence spectroscopy
… within a wide range between ~ 1 and 28 pN. Macromolecular complexes of proteins are ubiquitous in the cellular milieu. Their composition, stoichiometry, order of assembly etc. are intrinsic to their underlying functions. We combined the principles of conventional immunoprecipitation assays …
-
Component Trees For The Exploration Of Macromolecular Structures In Biology
… the three-dimensional structure of a macromolecular complex is essential for understanding its function. A component tree is a topological and geometric image descriptor that captures information regarding the structure of an image based on the connected components determined by …
-
LOCALIZED WNT3A-SOURCES INSTRUCT MITOTIC ORIENTATION: DISSECTING THE WNT3A-TRANSDUCTION MECHANISM COUPLED WITH NUMA MACHINERY
… consisting of dynein/NuMA/LGN/Gai cortical complexes that define the spindle alignment by exerting pulling forces on dynamic plus-ends of astral MTs to control spindle position and orientation. How these force generators respond to extracellular signal remains poorly characterised. In …
-
Global identification of SUMO modification sites
… with similar functions or found within the same macromolecular complexes. These data provide a comprehensive resource for future research on the physiological role of sumoylation in health and disease.
-
Role of communication between subunits and enzymes in ClpXP-mediated substrate unfolding and degradation
… proteins fold, by dismantling hyper-stable complexes, and by degrading unwanted proteins. The highly conserved AAA+ Clp/Hsp100 proteins are ATPases which function as disassembly chaperones as well as essential components of energy-dependent proteases. For example, the ClpX ATPase …
-
Femtosecond Time-Resolved Laser Spectroscopic Studies on the Electron Dynamics in Heterostructured Nanomaterials
… The electron transfer dynamics in organic macromolecular complexes are also of interest in this dissertation. The organic capsule named octa acid is the model system studied with femtosecond laser techniques. We believe that understanding the charge carrier dynamics in nano- and …
-
Deep learning methods to study structurally heterogeneous macromolecules in vitro and in situ
… carry out essential biological processes. These macromolecular complexes are often structurally heterogeneous, which is key to executing or regulating their specific biological functions. To understand the molecular mechanisms underpinning these biological functions, structural biologists aim to …
Page 1 of 3