Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"mRNPs"”.
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Utilization of Poly(A)-Binding Protein in Monitoring the Formation of mRNP Granules in Toxoplasma gondii
… of messenger ribonucleoprotein complex (mRNPs). I chose to study the poly(A) binding protein (PABP). Metazoans usually encode multiple isoforms of PABP to function in nucleus and/or cytoplasm but in Toxoplasma there is only one predicted PABP. In this thesis, I studied the localization of …
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Nanobody-Based Interactomic Studies of Single Transcripts During mRNA Maturation
… We thus sought to develop a method to isolate mRNPs from a single transcript, allowing us to study the dynamic RNP compositions of individual mRNA maturation pathways. To optimize purifications of the protein tags required for RNP isolations, we first generated high affinity reagents targeting …
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SYK promotes TGF-beta-induced P-body clearance in breast cancer cells through the enhancement of autophagy
… a variety of messenger ribonucleoproteins (mRNPs) as SYK substrates. A number of these mRNPs are associated with processing bodies (P-bodies), mRNP granules that are associated with mRNA degradation. These interactions led me to hypothesize that Syk co-localizes with P-bodies. I found that …
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Genetic, Biochemical, and Electron Microscopic Analysis of Components Involved in Transcription Coupled mRNA Export
… in the nucleus that couple formation of mRNPs with translocation through the pores. When I started my PhD work, I performed a synthetic lethal (sl) screen to investigate the role of Gle2 in mRNA export. Gle2 was a proposed to be an mRNA export factor. Thus, the aim has been to gain an …
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Papel de la helicasa UAP56/DDX39B y otros factores asociados al metabolismo del ARN en la integridad del genoma
… en forma de ribonucleoproteínas mensajeras (mRNPs) para la elongación de la transcripción, la integridad y el procesamiento del ARNm, previo a su transporte al citoplasma. Dicho ARNm, como sustrato potencial en la formación de R loops, ha de ser pertinentemente procesado, empaquetado y …
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Functional Roles of Nova in RNA Metabolism
… of Nova in heterogeneous structures, probably mRNPs, and with polysomes, suggesting a role for Nova in the regulation of cytoplasmic phenomena of RNA metabolism, such as mRNA localization and translation. These considerations prompted a gene expression screen aimed to identify differences in …
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Structural characterization of a protein/RNA complex : human TAP/NXF1 protein/retroviral CTE RNA
… packing it into ribonucleoprotein particles (mRNPs). In humans, the protein TAP/NXF1 interacts with mRNA export cargoes and mediates their nuclear export by shuttling through nuclear pore complexes (NPCs). TAP is a multidomain modular protein composed of an N-terminal mRNP-binding region and a …
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Ruolo della TDP-43 nella formazione dei granuli da stress nella Sclerosi Laterale Amiotrofica
… of non-translating messenger ribonucleoproteins (mRNPs) that rapidly aggregate in cells exposed to adverse environmental conditions. Stress granules function in part to triage RNA and sequester transcripts not needed for coping with the stress. We evaluated stress granule dynamics in primary …
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Multi-color visualization and quantification of single RNA translation and HIV-1 programmed ribosomal frameshifting in living cells
… between RNP granules. Thus, the recruitment of mRNPs to RNP granules involves dynamic, stable and extended interactions affected by translation status, mRNA length and granule size that collectively regulate RNP granule dynamics. Finally, NCT was utilized to quantify the dynamics of multiple …
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Etablierung und Optimierung der synthetischen primär-microRNA Technik für funktionale Genomanalysen in Arabidopsis thaliana
… in direkter oder indirekter Weise am Export von mRNPs beteiligt sind, höchstwahrscheinlich über den Exportin1-vermittelten Transportweg.
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Molecular mechanisms behind the adjustment of phototrophic light-harvesting and mixotrophic utilization of cellulosic carbon sources in Chlamydomonas reinhardtii
… silent messenger ribonucleoprotein complexes (mRNPs). The overexpression of NAB1 decreases LHCII protein amount whereas NAB1 knockout leads to an increased level of LHCII proteins. Consequently, NAB1 is part of a control system regulating the size and composition of the LHCII complex at the …
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Investigating the function and mechanistic control of processing bodies in early Drosophila melanogaster development
… that either affected the ability of P-body mRNPs to condense into larger bodies or altered the physical properties of P-bodies, which in turn affected their ability to retain *bcd*. In mature oocytes, when *bcd* fails to localise or condense into P-bodies either through genetic or chemical …
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The role of human and Drosophila NXF proteins in nuclear mRNA export
… to facilitate the interaction of NXF1:p15 with mRNPs. Even though both NXF1:p15 heterodimers and UAP56 had been implicated in general mRNA export, it was unclear whether there are classes of mRNAs that require NXF1:p15, but not UAP56 or vice versa. It was also unclear what fraction of cellular …