Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"mRNA-seq"”.
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Transcript Diversity In The Protozoan Parasite Toxoplasma Gondii
Technological advances have made possible to sequence RNA transcripts at unprecedented depth, enabling deep profiling of abundance and diversity under a variety of conditions. Such information permits refinement of draft genome annotation originally generated in the absence of transcript coverage …
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Genome-Wide Characterization of the Effects of Nucleic Acid Modifying Enzymes: Cytidine Deaminases and DNA Methylation
… of these cases, the advent of high-throughput sequencing provides methods for genome-wide characterization of AID's effects. This thesis presents the application of a number of genome-scale, sequencing-based methods to characterize the effects of AID deficiency and overexpression on the …
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A study of mammalian microRNA-mediated repression of gene expression by ribosome profiling
… gene expression by base pairing to their target mRNAs to direct repression. In animals, this repression is usually mediated through translational repression and/or mRNA destabilization. In studies that investigate miRNA-mediated repression with reporter constructs or individual endogenous genes, …
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Molecular characterization of CLE peptide mimicry during cyst nematode pathogenesis
… laser capture microdissection coupled with mRNA-Seq to identify downstream signaling cascades after nematode CLE perception. The results of these studies will contribute to our understanding of how plant-parasitic nematodes interact with their host plants on a molecular level, and could open …
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Characterizing the Nuclear Function of the Cell Adhesion Molecule Fat and Chromatin Regulation by the Inner Nuclear Membrane Protein Speg
… to specific genomic loci in vivo. Through ChIP-seq, I characterized the genome-wide DNA binding profile of Ft in vivo. ChIP-seq results suggested that nuclear Ft binds mostly open chromatin. Interestingly, the ChIP-seq profile of Ft is highly correlated with that of the Hippo pathway …
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Mapping PAMP Responses and Disease Resistance in Brassicas
… PAMP-Induced resistance. Illumina-based RNA sequencing analysis produced a genome-wide survey of transcriptional changes upon PAMP treatment seen in both the A and C genomes of the allotetraploid B. napus. Using these assays substantial variation in PAMP-responsiveness was observed amongst …
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Identification of RBPMS as a smooth muscle master splicing regulator via association of its gene with super-enhancers
… changes during this transition as indicated by mRNA-Seq of rat PAC1 cells with RBPMS-manipulated levels. RBPMS overexpression also promoted splicing events that are usually only observed in tissue SMCs. RBPMS targeted a network of proteins involved in the cytoskeleton and cell-adhesions, …
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Quality checking and expression analysis of high-throughput small RNA sequencing data
The advent of high-throughput RNA sequencing (RNA-seq) methods have made it possible to sequence transcriptomes for the cell-wide identi�cation of small non-coding RNAs (sRNAs) and to assess their regulation using di�erential expression analysis by comparing two or more di�erent conditions. During …
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THE EPITRANSCRIPTOMIC REGULATION OF STRESS: ROLE OF N6-METHYLADENOSINE IN ABIOTIC STRESS RESPONSE
… the most prevalent internal mRNA modification, can alter gene expression by affecting the stability, processing, and translation of mRNA among other functions. Very few studies have explored the involvement of m6A in the epitranscriptomic regulation in a physiological context, …
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Characterization of activation tagged potato (Solanum tuberosum L.) mutants
… transcriptional and regulatory pathways. The mRNA-seq analyses resulted in 1,632 genes that were differentially expressed between mutant up and Bintje and the total number of up-regulated genes (661) were less than the number of genes down-regulated (971 genes) in the up mutant. Further …
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Functional study of miRNA-mRNA interactions in malaria mosquito An. gambiae
… of miRNAs in reproduction, I used small RNAs sequencing to monitor miRNAs expression and their association with Argonaute 1 (Ago1) and Argonaute 2 (Ago2) in the malaria mosquito Anopheles gambiae (An. gambiae) during the 72-h period immediately after blood feeding. I found the abundance and …
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Role of virus genes in seed and aphid transmission and development of a virus-induced gene silencing system to study seed development in soybean
… The small RNA (21-24 nucleotide size) deep sequencing of TSV-infected soybean plants with symptom recovery revealed that virus derived small interfering (vsi)RNA originated from hot spots within the TSV genome as indicated by the number of mapped sequences. The vsiRNA hot spots were not …