Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 68 for “"mRNA translation"”.
-
SOMATIC SIGNALS REGULATING MRNA TRANSLATION IN MAMMALIAN OOCYTE.
… mechanisms on a background of repressed mRNA transcription. However, a comprehensive understanding of this regulation in higher-order mammals has been limited by challenges in isolating polysome-associated mRNAs. This study aims to shed light on these processes and how they can be …
-
Rbfox1 Regulates mRNA Translation to Promote Germ Cell Differentiation
… We further show that Rbfox1 represses the translation of mRNAs that contain (U)GCAUG elements within their 3’ UTRs. We have identified pumilio (pum) as a critical Rbfox1 target gene. Pum is an RNA-binding protein essential for germline maintenance across species. During germline cyst …
-
Bruno regulates mRNA translation by binding to multiple sequence motifs
… in Drosophila melanogaster oocytes. oskar (osk) mRNA is translationally repressed until it reaches the posterior of the oocyte where Osk protein accumulates. Translational repression of osk prior to posterior localization is mediated by the RNA binding protein, Bruno (Bru). To better define Bru …
-
An investigation of nutrition-dependent mRNA translation in Drosophila larvae
… for sustained growth downstream of nutrition is mRNA translation. In order to coordinate cellular and organismal growth with nutrient availability, organisms have devised several control checkpoints to limit the energy consuming process of translation under starvation. Work on mammalian cell …
-
Mitochondrial mRNA Translation is Required for Maintenance of Oxidative Capacity
… accomplish this may be through targeting the mt-mRNA translation system. Purpose: This investigation’s purpose was to characterize disruptions in mt-mRNA translation machinery in multiple forms of cardiomyopathy and to determine if mitochondrial mRNA translation initiation factor (mtIF2) is …
-
Iron regulates mRNA translation initiation through RNA iron responsive element (IRE)
… (IRP) proteins inhibits ribosome accessing mRNA. Increasing iron concentration decreases IRP binding with IRE-RNA. Previous models of IRE-mRNA translation regulation concentrate on Fe-S binding to IRP and IRP degradation after release from IRE-RNA. These models lack information on the …
-
A study of mRNA translation with computational analysis of ribosome profiling datasets
… is based on capturing and sequencing of the mRNA fragments enclosed within the translating ribosome and it thereby provides a “snapshot” of ribosome positions at the transcriptome wide level. The approach was developed in 2009 and was a significant advancement towards the better understanding …
-
Dissecting the role of RNA helicase DDX3X in mRNA translation and B-cell function during immune responses
… the effect of MYC on the upregulation of global translation, ultimately lowering the levels of proteotoxic stress and enabling lymphoma development. These findings suggest the functions of MYC and DDX3X are related but how this occurs in the context of physiological B-cell responses has remained …
-
Understanding the Role of Hypusine Biosynthesis in Endocrine-Exocrine Crosstalk
… pancreatic growth and health. Additionally, translational control mechanisms have been linked to organ development. Our lab has shown that the mRNA translation factor eukaryotic initiation factor 5A (eIF5A), when in its post-translationally modified “hypusinated” form, plays a role in …
-
Translational Control by the Ribosome-Associated Complex in the Unfolded Protein Response
… role of de novo protein folding with translation. Deletion of RAC causes growth defects and sensitizes to osmotic, cold, and aminoglycoside stresses in yeast. Furthermore, studies have shown that Mpp11 is over-expressed in head and neck squamous cell cancer and leukemia. However, the …
-
The role of cellular signalling pathways and translation initiation factors in Herpesvirus infection
… the role of host cell signalling and translation initiation during reactivation from a non productive state. To achieve these objectives, a new system was established to study HSV-1 quiescence. The system employs the use of serum starved and temperature elevated primary human cells …
-
Design, Off-Target Toxicity, and Intracellular Processing of Nucleotide-Modified Messenger RNAs for Human Therapies
… to gene therapy, in which in vitro-transcribed mRNA (IVT mRNA) is introduced to cells for therapeutic applications. IVT mRNA is transfected into target cells, translated, and the fully-synthesised encoded protein exerts a therapeutic effect via specific mechanisms. IVT mRNAs have been applied to …
-
Development of a ribosome profiling protocol to study translation in the yeast Kluyveromyces marxianus
During mRNA translation, the ribosome protects ~28 nt of mRNA within its mRNA tunnel. Ribosome profiling is a method which takes advantage of this protected mRNA fragment, commonly referred to as the ribosome protected fragment (RPF). This method uses endonucleases to digest unprotected mRNA, …
-
Mining common genetic Variants impacting on Allele-Specific Translation and cancer risk
The mRNA translation regulation is a critical factor in protein expression and cellular homeostasis, with deep implications for cancer biology. The work described in this thesis identifies, validates, and investigates allele-specific differences in translation efficiency mediated by tranSNPs: …
-
A Gain-of-Function Role of Apolipoprotein E2 in Melanoma Progression
… from this genetic model revealed upregulation of mRNA translation in APOE2 melanomas relative to APOE4 melanomas. After experimental examination of the effects of APOE variants on melanoma translational efficiency, I report the discovery that APOE2 acts in a gain-of function manner to enhance …
-
A multi-level approach to understanding the regulation of translation initiation
mRNA translation is an extremely complex process required for life. Translation consumes vast amounts of cellular resources, and organisms have evolved tight regulatory mechanisms to control this process, which are often deregulated in cancer and other disease states. Initiation, as the …
-
Modulation of RNA Cytosine-5 Methylation by Neuronal Activity and Methyl-donor Folate
… important epitranscriptomic mark that modulates mRNA transportation, stability and translation. In chapter 1, we summarize the currently available approaches to detect 5-mrC modification at global, transcriptome-wide and locus-specific levels, and compare the corresponding advantages and …
-
Integrating discrete stochastic models with single-cell and single-molecule experiments
… such as stochastic gene expression and single-mRNA translation. The methods developed here emphasize a strong link between model and experiment to help understand, design, and eventually control biological systems at the single-cell level.
-
Deconvoluting Cell-Type Specific 3'UTR Isoform Expression in the Adult and Developing Cerebellum
… has been implicated in the regulation of mRNA translation and stability, as well as mRNA and protein localization. However, it is unclear to what extent alternative polyadenylation regulates these processes uniquely in specific cell types. Using a newly developed technique, termed …
-
Investigating the translational and metabolic reprogramming of the bone marrow niche in myeloid malignancy
… cells surviving chemotherapy rely on high mRNA translation and their microenvironmental metabolic support to drive relapse. However, the role of translational reprogramming in the niche was, until now, unclear. This study found that relapsing AML cells increase translation in their bone …
Page 1 of 4