Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"mRNA transcription"”.
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Comparative study of the kinetics and mechanisms of adenovirus and bacteriophage PR772 inactivation by disinfectants used for global safe drinking water
… but genome synthesis as well as late hexon mRNA and early E1A mRNA transcription were inhibited. When exposed to free chlorine, PR772 had remarkably similar inactivation kinetics compared to HAdV-2. Like HAdV-2, free chlorine inactivated PR772 could still bind to host cells but genome …
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The hepatitis B core promoter: Control of viral life cycle events
… both the pre-core and the pre-genomic mRNAfrom which translation of the HBeAg and HBcAg occurs respectively..This study aimed to investigate the importance of the CP in viral replication. Pairedsamples, pre- and post HBeAg seroconversion, were selected from 14 chronic HBVpatients who …
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Staphylococcus aureus as a source of antigens stimulating bovine dendritic cells and lymphocytes in vitro
… loaded with either ISA or LSA induced increased mRNA transcription of Th17-related cytokines and cytotoxic effector memory cell formation during antigen recall experiments. Lymphocytes co-cultured with LSA-loaded DC exhibited a higher fold-change in interferon (IFN) γ mRNA compared to ISA-loaded …
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Adenovirus-based exogenous gene expression in mammalian cells
… and stability inside the cells as well as mRNA transcription and protein expression. First, the effect of DNA quality on its delivery, stability and expreSSIOn III mammalian cells was studied. Five different conditions of the major DNA contaminants were used in this investigation including …
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Discovering non-equilibrium mechanisms that regulatestructure and function of biomolecular condensates usingphase-field modeling
… in biomolecular condensates associated with RNA transcription undergo complex coacervation with the RNA product. The first project in this thesis investigates the interplay between complex coacervation and spatially heterogeneous RNA synthesis on condensate morphology and dynamics using a …
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Identification and functional evaluation of a bioactive peptide QUB-1369 Secreted from the skin of The Common Frog (Rana Temporaria)
… temporaria, was used to build a cDNA library by mRNA transcription. A cDNA encoding a peptide biosynthetic precursor transcript was consistently cloned from this by “shot-gun” cloning. The precursor peptide sequence was analysed by bioinformatics tools such as NCBI-BLAST, while Uniprot identified …
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CRISPR-based Transcriptional Activators for Dissecting Human Gene-Regulatory Mechanisms
… human CBP and GCN5 and tested their efficacy as transcriptional activators. For the optimization of the dCas9-toolbox, we showed that affinity-purification-mass spectrometry could be applied to identify the antagonistic gene for these dCas9-based tools in the cellular context, which enables us to …
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Computational Approaches to Predict Effect of Epigenetic Modifications on Transcriptional Regulation of Gene Expression
… protein-DNA bindings. Such proteins are known as transcription factors. Transcription is the first step of gene expression where a particular segment of DNA is copied into the messenger-RNA (mRNA). Transcription factors orchestrate gene activity and are crucial for normal cell function in any …
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Development and Evaluation of Nano‑scale Systems for Targeted Delivery to Treat Liver Fibrosis
… administrated to prevent type I collagen mRNA transcription. To enhance circulation time and targeted delivery efficiency, TFO was conjugated to M6P‑HPMA and showed efficient targeted delivery to HSCs. Two week short term in vivo i.v. administration also showed the therapeutic effects on …
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Identification and Molecular Cloning of A Bioactive Peptide from the Defensive Skin Secretion of the Purple-Sided Leaf Frog, Phyllomedusa baltea
… of genomic and proteomic analysis. The mRNA was isolated from the skin secretion and used to establish a cDNA library by mRNA transcription. The target cDNA of interest was amplified and sequenced using a typical procedure of molecular cloning. On the other hand, MS/MS fragmentation …
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Cellular Reprogramming in Skeletal Muscle after Repeated Exposures to Endotoxin
… was assessed by measuring: 1) changes in mRNA expression of interleukin-6 (IL-6) and monocyte chemoattractant-1 (MCP-1) as markers of a pro-inflammatory response; and 2) mRNA levels of peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC1-°) and mitochondrial oxidative …
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The enzymatic function of the CCR4 protein
… 1.9 and 1.0 x 10 6 MDa in size, is a general transcriptional regulatory complex. It regulates mRNA transcription in initiation, elongation and degradation.</p><p>The 1.0 MDa complex was purified to near homogeneity, and mass spectrometric analysis was used to identify all the components of the …
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Tissue specific genetic regulation of Interleukin 6
… were genotyped for SNP G-174C. A new pre-mRNA assay was developed, using gel electrophoresis, restriction digest and fluorescence quantification, to measure the ratio of heterozygous allelic pre-mRNA transcription. IL6 mRNA expression in different tissues was also measured using relative …
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Cytochrome P450 mRNA profile in human breast cancer cell lines.
… P450 and P450-regulatory nuclear receptor mRNAs in a series of breast cancer cell lines (BCCs), and secondly to compare this profile with normal breast cells. This was achieved using a qualitative reverse transcriptase-polymerase chain reaction (RT-PCR) to detect mRNA expression of target …
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Investigating the Role of the mRNA Capping Enzymes in Mouse Embryonic Stem Cells and Differentiation
The mRNA cap consists of a series of co-transcriptional modifications on nascent transcripts that confer protection from degradation and mediate RNA processing and translation. The addition of an inverted guanosine cap to the 5’-end of the transcript and its subsequent methylation along with the …
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Molecular characterization of three linked genes, fixB, βshbd and adh1 from Clostridium acetobutylicum P262
… the expression of these linked genes by transcriptional analysis. The upstream region of the βhbd gene was isolated from a gene bank of C. acetobutylicum P262, constructed using the pWE15 cosmid vector. Characterization of this upstream region was done initially at the nucleotide sequence …
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Enhancing Chondrocyte Biosynthetic Activities: Harnessing the Power of Loading-Induced Evolved Temperature
… cell encapsulation, as illustrated by enhanced mRNA transcription and synthesis of cartilage-related proteins. Next, through external application of load, heat, and hypoxia, we observe synergistic enhancements in chondrocyte biosynthetic activity (predominantly in collagen and aggrecan …
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Proteomics and dynamics of the human nucleolus
… entry. The NHPX progression is dependent on pre-mRNA transcription and may link multiple RNA metabolic pathways that occur in distinct subnuclear domains.
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Appendage development and early Distal-less regulation in arthropods: A study of the chelicerate Tetranychus urticae (Acarida)
… to provide a basis for understanding mRNA transcription and protein activity patterns, I confirmed typical expression of Tetranychus Dll in prosomal limb primordia. I obtained limited results for the candidate antero-posterior positioning genes wingless and engrailed, although one of …
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Identification of determinants of sensitivity to duocarmycin analogues: A class of potent anti-cancer drugs
… (G1-S phase transition), and RNA processing (mRNA transcription regulation) as key processes related to sensitivity to duocarmycin analogues. Differential gene expression analysis between sensitive and resistant cell lines as defined by the IC50 data was also carried out identifying RAD54 and …
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