Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 54 for “"mRNA processing"”.
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Nonsense-mediated mRNA reduction and pre-mRNA processing in drosophila
… mutation causes a reduction in the level of the mRNA of the corresponding gene. The reduction is not, contrary to initial expectations, due to a passive mechanism by which non translated mRNAs are degraded; rather it is a active process in which active translation, cis-acting sequences and …
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Detecting Changes in Alternative mRNA Processing From Microarray Expression Data
<p>Alternative mRNA processing can result in the generation of multiple, qualitatively different RNA transcripts from the same gene and is a powerful engine of complexity in higher organisms. Recent deep sequencing studies have indicated that essentially all human genes containing more than a …
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Functions of Deadenylation Factors In Mrna Decay and Mrna Processing Body Formation
… because deadenylation not only leads to mRNA translational inhibition but also is the first step of major mRNA degradation. Translationally inhibited mRNAs can be stored and/or degraded in dynamic cytoplasmic foci termed mRNA processing bodies, or P bodies, which are conserved in …
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Integrative analysis of transcriptomic data to elucidate regulators of pre-mRNA processing
Alternative pre-mRNA processing events, including alternative splicing (AS) and alternative polyadenylation (APA), are key drivers of transcriptomic and proteomic diversity. This dissertation presents a comprehensive framework for analyzing these events, emphasizing improvements to computational …
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Crystallographic studies on a cold adapted subtilase and proteins involved in mRNA processing
Die Kristallstruktur einer Subtilisin-ähnlichen Serin-Proteinase des psychrotrophen marinen Bakteriums, Vibrio sp. PA-44, wurde durch molekularen Ersatz bei 1,84 Å Auflösung aufgeklärt. Damit konnte zum ersten Mal die Struktur einer kälteadaptiven Subtilase aufgeklärt werden. Dies macht es möglich, …
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Diverse primary and secondary structural features are associated with Y complex-dependent mRNA maturation in B. subtilis
In Bacillus subtilis, mRNA processing and decay are primarily initiated by the endonuclease RNase Y. Recent work by our group discovered a set of auxiliary factors called the Y complex that is required for the mRNA maturation of 21 operons. It is unclear, however, how these operons specifically are …
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Functional and computational analysis of RNA-binding proteins and their roles in cancer
This work is concerned with mRNA processing in mammalian cells and proceeds in two parts. In the first part, I introduce a computational framework for inferring the abundances of mRNA isoforms using high-throughput RNA sequencing data. This framework was applied to study the targets of the …
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Polymorphisms in the Flt1 gene and their relation to expression of the secreted Flt1 variant
… form of the receptor (sFlt1) translated from an mRNA in which a portion of intron 13 is preserved. sFlt1 retains high affinity for VEGF and thereby inhibits its angiogenic activity. Intron 13 contains important cis elements involved in sFlt1 mRNA formation. Here, we test the hypothesis that …
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Multiple Levels of Regulation of Cyclin-dependent Kinase Inhibitor 1A (p21/WAF-1) by the <i>DDX5 </i>Gene
… biogenesis, transcription, RNA decay, miRNA processing and pre-mRNA processing. Alterations in both expression and functional activity of p68 have been linked to carcinogenesis, with p68 exhibiting both tumour suppressive and oncogenic properties, highlighting the extremely contextual nature …
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Localization of Small-Nuclear-Rna Genes of Drosophila Melanogaster on Polytene Chromosomes
… the suggestion that snRNAs act as mediators in mRNA processing. However, there is no direct evidence.
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New Roles in RNA Stability and Transcription for the RNA Export Protein, REF/Aly
Pre-mRNA processing consists of a series of events including capping, splicing, and polyadenylation, which occur co-transcriptionally, are interdependent, and are coupled to other steps in gene expression including transcription and RNA export. For example, in mammalian cells the TREX …
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Regulation of APOBEC3 activity and HIV-1 replication by P-body associated proteins
… in miRNA mediated translational repression and mRNA decay. Components of this pathway, as well as A3G and silenced mRNAs have been shown to localise to discrete cytoplasmic foci termed mRNA Processing (P) Bodies. These foci have very recently been implicated in influencing viral life cycles. …
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A Proteomic Analysis of Differentiation in the Mammary Epithelium
… in proteins related to structural dynamics and mRNA processing within these two mammary epithelial cell types. Intriguingly, we observed several differences in the regulation of metabolic proteins, highlighting the distinct pathways by which different species probably metabolize energy and …
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Characterisation of the multi-isomeric protein nesprin-1 in p-body and mRNA dynamics
… promoter usage and alternative 3’ end processing, the nesprins are able to generate multiple mRNA transcripts, leading to the production of diverse tissue specific isoforms with potential roles beyond the NE. To explore further the capacity of nesprin-1 to generate alternative …
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Nanobody-Based Interactomic Studies of Single Transcripts During mRNA Maturation
… transcription in the nucleus, messenger RNAs (mRNAs) undergo a variety of processing events before being exported to the cytoplasm through the nuclear pore complex. mRNA processing and nuclear export require a wide range of protein factors, which interact with maturing transcripts and each …
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Purification, characterization and substrate specificity of a nuclear mRNA (Guanine-7)-Methyltransferase from Ehrlich ascites cells
The 5' - termini of most eukaryotic and viral mRNAs contain the sequence of m7G(5')ppp(5')N- known as the "cap" structure. The guanine-7 methylation of this cap structure is important for mRNA processing and initiation of translation. A RNA (guanine-7)-methyltransferase that specifically methylates …
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ARCHITECTURE AND RNA RECOGNITION BY THE EUKARYOTIC M6A METHYLTRANSFERASE COMPLEX
… (m6A) is one of the most abundant mRNA modifications and contributes to key cellular processes, including mRNA processing, translation, and decay. In eukaryotes, m6A is installed by the m6A Methyltransferase Complex (MTC), a multi-protein assembly composed of seven subunits in …
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Role of PBRM1 in regulation of the HIF pathway
… PBRM1 is important for recognition of HIF1α mRNA by YTHDF2 and able to interact with mRNA suggesting a link between this chromatin remodeling complex and mRNA processing. Additionally, PBRM1 contributes to the hypoxia induced expression of HK2 and PHD2 genes, possibly by interacting with HIF1 …
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Cell cycle-dependent regulation of the human RNA cap methyltransferase (RNMT)
… gene expression including transcription, pre-mRNA processing, RNA export and translation. The N-7 methylguanosine cap is added co-transcriptionally to RNA pol II transcripts. In mammals, two enzymes catalyse the synthesis of the N7-methylguanosince cap. RNGTT adds an inverted guanosine group …
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Structural characterization of a protein/RNA complex : human TAP/NXF1 protein/retroviral CTE RNA
… including transcription, post-transcriptional processing of pre-mRNA transcripts and export of the correctly processed mRNAs from the nucleus to the cytoplasm, where translation takes place. Evidence has accumulated suggesting that these steps are both functionally and physically coupled by …
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