Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"mRNA metabolism"”.
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From cradle to grave: The influence of mRNA metabolism on plant stress responses
… As the intermediary between DNA and protein, mRNA is a crucial cog in plant gene expression, and consequently a key regulatory target. During stress, control of mRNA metabolism (and downstream protein production) takes on particular importance due to the energy constraints imposed on the cell. …
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From cradle to grave: The influence of mRNA metabolism on plant stress responses
… As the intermediary between DNA and protein, mRNA is a crucial cog in plant gene expression, and consequently a key regulatory target. During stress, control of mRNA metabolism (and downstream protein production) takes on particular importance due to the energy constraints imposed on the cell. …
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Form and function of Poly(A) tails
Central to mRNA metabolism is the poly(A)-tail, a stretch of adenosine nucleotides at the mRNA 3' end. In this dissertation, I investigate the role of the tail in the dynamics of mRNA decay, and describe the predominant mechanisms of decay for thousands of mammalian mRNAs. Next, I examine the …
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Global analysis of mRNA decay rates and RNA-binding specificity reveals novel roles for CUGBP1 and PARN deadenylase in muscle cells
… and MBNL1 are disrupted, resulting in altered mRNA metabolism at the levels of splicing and translation. Intriguingly, despite strong evidence for CUGBP1 being a regulator of mRNA turnover in humans and other organisms, the possibility that defects in mRNA decay contribute to DM1 pathogenesis …
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Stress-Induced Targeting of Molecular Chaperones In The Yeast Saccharomyces Cerevisiae
… bodies (P- bodies) and stress granules, sites of mRNA metabolism. I found that Hsp104 recruits both Ssa1 and Hsp26 to P-bodies and that these three chaperones are required for stress granule formation. These findings suggest a possible role for chaperones in mRNA metabolism by aiding in the …
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KSHV encoded viral processivity factor, ORF59, regulates multiple pathways for viral replication
… (NCL), involved in ribosome assembly, pre-mRNA metabolism, cytoplasmic RNA stability, and nucleo-cytoplasmic transport as well as ORF57, an mRNA transcript accumulation (MTA) viral early protein involved in posttranscriptional regulation, splicing of viral RNA transcripts, RNA stability, …
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Spinal Muscular Atrophy: Evidence of a Multi-System Disease
… protein) has critical and ubiquitous roles in mRNA splicing, amongst various other functions in mRNA metabolism. As such, SMN depletion in other non-neuronal cells type is likely to have physiological repercussions, and perhaps modulate the SMA phenotype. Herein, we identify the molecular …
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Functional characterization of the RNA binding protein RALY
… many steps of biological processes regarding RNA metabolism. The heterogeneous nuclear ribonucleoproteins (hnRNPs) belong to big family of RBPs involved in many aspects of RNA metabolism including RNA stability, intracellular transport and translation. More recently, RALY, a RNA-binding protein …
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Bioinformatics study of mammalian MRNA polydenylation
… cells, which have influences on many aspects of mRNA metabolism. Several human diseases have been shown to associate with abnormal polyadenylation, highlighting the importance of this process. The availability of the complete sequence of human and mouse genomes, together with their gene …
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Mechanisms of persistent translation arrest following global brain ischemia and reperfusion
… studies, my results indicate that the mRNA granules are ribonomic structures involved with mRNA regulation. This finding is significant because it shifts the focus onto mRNA metabolism and away from ribosomal molecular biology. I have identified new pathways to investigate for …
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Function and regulation of poly(A)-tail length
… 3' ends of nearly all eukaryotic messenger RNAs (mRNAs) and long non-coding RNAs. The presence of a poly(A) tail promotes translation and inhibits decay of an mRNA, with both effects mediated through poly(A)-binding protein. However, an understanding of the relationship between the length of a …
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The regulation and function of mRNA cap methylation in pluripotency and differentiation
… the N7-methylguanosine cap at the 5’ end of pre-mRNA occurs co-transcriptionally and is catalysed by a series of enzymes including the N7 RNA methyltransferase (RNMT), which along with its recently discovered activating subunit RAM, methylates the cap. RAM, which contains an RNA binding domain, …
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Structure-Function Relationships of Elements of the Bacteriophage f1 Genome
… requirements of specific regions involved in f1 mRNA metabolism were investigated by: 1) relocation of an intact transcription unit on the phage genome; 2) construction of a hybrid E. coli-f1 transcription unit which could be studied in a phage or plasmid context; 3) identification and …
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Mechanisms of translation arrest following focal brain ischemia
… formation of subcellular particles involved in mRNA metabolism such as stress granules and mRNA granules. My Background discussion will lead to my hypotheses about mechanisms of prolonged TA following focal brain I/R and the possible effect of diabetes on these mechanisms. In subsequent chapters …
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Modulation of Stress Granules formation: Role of mGlu5 receptor and FMRP and implications for pathophysiology of Fragile X Syndrome
… FMRP is an RNA-binding protein involved in mRNA transport and translation. Despite numerous studies, the available treatments are only symptomatic. There is no cure to replace FMRP expression, yet. FMRP can interact with RNA-binding proteins such as FXR1P, FXR2P, NUFIP and 82-FIP, and with …
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Characterizing novel molecular regulators of antiviral gene expression
… (m6A), which regulates many aspects of mRNA metabolism through transcript-specific effects. m6A deposition is mediated by a cellular complex of proteins including METTL3 and METTL14 (METTL3/14) and other cofactors, and m6A can also be removed from RNA by the demethylase proteins FTO and …
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Commonalities between SMA and ALS: investigation of ribosome heterogeneity and translational defects
… capable of finely tuning translation of selected mRNAs, do exist. Multiple types of ribosomes are defined by several elements, such as their composition, and their association with different proteins, known as ribosome-associated proteins (RAPs). In particular, these proteins can exert a direct …