Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"mRNA export"”.
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Investigating Mechanisms of Nuclear mRNA Export
… cytoplasm has important implications for nuclear export and retention of mRNAs. This division ensures that only correctly processed mRNAs are exported to the cytoplasm to be translated into proteins, while misprocessed and aberrant transcripts are retained in the nucleus to be degraded by various …
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A link between c. Elegans morphogenesis and mrna export
… thermo-sensitive t2160 mutant allele of nuclear export 1, nxf-1. nxf-1/TAP encodes a protein that is required for mRNA export from the nucleus to the cytoplasm. To better understand the role of NXF-1 in C. elegans embryonic morphogenesis, we analysed the expression of several apical junction …
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ELUCIDATION OF REGULATORY MECHANISMS OF TRANSCRIPTION AND COUPLED mRNA EXPORT
… the central processes in gene expression is the export of the synthesized mRNA from nucleus to the cytoplasm where it gets translated into proteins. This journey of mRNA is mediated by numerous factors and receptors that get loaded onto the transcribing mRNA in a co-transcriptional fashion. …
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The role of human and Drosophila NXF proteins in nuclear mRNA export
… cells is the spatial separation of the site of mRNA synthesis (nucleus) from the site of mRNA function (cytoplasm) by the nuclear envelope. As a consequence, mRNAs need to be actively exported from the nucleus to the cytoplasm. At the time when this study was initiated, both human TAP and yeast …
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Biochemical and Structural Analysis of the Nucleoporin Nup214 and its Involvement in mRNA Export
… sense of the architecture and regulation of the mRNA export machinery at the NPC, I set out to biochemically and structurally characterize Nup214. In this thesis, I present the crystal structure of the human Nup214 N-terminal domain at 1.65 Å resolution. The structure reveals a sevenbladed …
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Genetic, Biochemical, and Electron Microscopic Analysis of Components Involved in Transcription Coupled mRNA Export
… between these compartments. Import and export of protein and RNA through the NPCs are highly regulated and follow several different pathways. Messenger RNAs (mRNAs) are exported from the nucleus only after extensive processing and assembly into ribonucleoprotein particles (RNPs). Studies …
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Characterization of the Association of mRNA Export Factor Yra1 with the C-terminal Domain of RNA Polymerase II in vivo and in vitro
… facilitates their localization to nascent pre-mRNA during transcription. In <italic>Saccharomyces cerevisiae</italic>, the mRNA export protein Yra1 (ALY/REF in metazoa) has been shown to cotranscriptionally associate with mRNA and is thought to deliver it to the nuclear pore complex for export …
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The Influenza A Virus NS1 Protein and Vriral mRNA Nuclear Export
… which means both the viral genome (vRNA) and mRNA must be exported from the nucleus to the cytoplasm. The mechanism by which vRNA nuclear export is achieved has been well characterised, but how viral mRNAs are exported is poorly understood. The cellular NXF1-dependent mRNA export pathway has …
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Studies on the interaction of HSV-1 multifunctional protein ICP27 with the nuclear pore complex proteins
… both a nuclear localization signal and a nuclear export signal and shuttles between the nucleus and the cytoplasm. It interacts directly with various cellular proteins including REF and cellular mRNA export receptor TAP to export viral mRNAs. However other transport mechanisms may also be …
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Untersuchung des CRM1-abhängigen RNA-Exports mittels Repräsentativer Differenz-Analyse
… anhand bestimmter Transportmotive. CRM1 ist ein Export-Rezeptor, der seine Substrate über Leucin-reiche Kern-Export-Signale bindet und daraufhin exportiert. Dieser Transport-Rezeptor hat ein weites Spektrum an Export-Substraten, da es sowohl Proteine als auch Protein-RNA-Komplexe wie ribosomale …
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Structural characterization of a protein/RNA complex : human TAP/NXF1 protein/retroviral CTE RNA
… post-transcriptional processing of pre-mRNA transcripts and export of the correctly processed mRNAs from the nucleus to the cytoplasm, where translation takes place. Evidence has accumulated suggesting that these steps are both functionally and physically coupled by protein-protein …
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The Role of NS1-BP in Influenza Virus Replication
… gene expression. I have shown that NS1 binds an mRNA complex containing NXF1/TAP, NXT/p15, Rae1, and E1B-AP5, which are key components of the mRNA export machinery. By targeting this complex, NS1 blocks host mRNA export, and cells become highly permissive to viral replication. Another …
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RNA EXPORT FACTOR ELF1 MAINTAINS GENOME STABILITY IN FISSION YEAST
… that Elf1, a AAA+ family ATPase involved in mRNA nuclear export, prevents genome instability. Upon deletion, elf1Δ cells grow slowly, forming small colonies. Curiously, they can switch to larger, faster-growing colonies. Genetic analysis revealed that frequent mutations, rather than prions or …
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Proteomic Identification of Histone Post-Translational Modifications Induced By Dna Double-Strand Breaks and Novel Proteins Involved In The Dna Damage Response
… the RNA pol II degradation factor Def1, the mRNA export protein Yra1 and the HECT E3 ligase Tom1. Each of these proteins was required for resistance to radiomimetics. Yra1 and Def1 were required for DSB repair per se, while Sit4 was required for rapid inactivation of the DNA damage checkpoint …
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Human FRG1 is an Actin-Bundling and a RNA-Associated Protein with Distinct Subcellular Localizations
… specifically with RNA in vitro, associates with mRNA in vivo, and directly interacts with the TAP mRNA export receptor. FRG1 also exists in a cytoplasmic pool that is dependent on an intact actin cytoskeleton for its localization and we demonstrate FRG1 itself is an actin binding protein. These …
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Characterization of Nuclear Localization Signals of Karyopherin-Mediated Nuclear Import
… the cytoplasm and significantly compromised its mRNA export function. Sequence examination of NXF1 from divergent eukaryotes and the interactions of NXF1 homologs with various Karyopherins have revealed the redundancy of nuclear import pathways for NXF1 increased progressively from fungi to …
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Analyzing the effects of Cis-elements and trans-factors on the stability of the Gal1 mRNP
… eukaryotic protein involved in transcription, mRNA export and perinuclear gene repositioning. Here, we show that the functions of Sus1p in perinuclear repositioning of GAL genes and its chromatin-linked functions can be genetically uncoupled, and that the role of Sus1p in the retention of mRNA …
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Investigating the cellular localisation of DEAD box helicase DDX3
… transcription, translation, splicing and mRNA export. DDX3 has also been shown to play a role in innate immune signalling and tumorigenesis. DDX3 is targeted by multiple viruses: HIV, HCV , HBV and Vaccinia Virus have all been shown to interact with DDX3; either using DDX3 to replicate or …
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Determinants of piRNA precursor transcription and export in Drosophila melanogaster
… These features are essential for canonical mRNA export mediated by Nuclear export factor 1, suggesting that piRNA precursor export requires an alternative machinery. In the second part of this thesis, I show that dual-strand cluster precursor export requires the Drosophila Nuclear export …
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Characterization of the Interactions Between Kaposi’s Sarcoma-Associated Herpesvirus ORF57 and Its RNA Partners
… levels including transcription, splicing, mRNA export, RNA stability, and translation. At least in some cases, direct interactions between ORF57 and its target RNAs are necessary for ORF57-mediated upregulation of viral gene expression. The work highlighted in this document reveals our …
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