Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"mRNA display"”.
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Development of Natural Cyclic Peptide Inhibitors of XRCC4/XLF Interaction for Radio-Sensitization of Breast Tumor Cells
… near M61 and F106 can be identified through an mRNA display in vitro selection, and these peptides will inhibit NHEJ and thereby radiosensitize breast tumor cells. We have synthesized five DNA libraries that produced mRNA-peptide fusions containing a trillion unique peptide sequences that will …
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Directed Evolution of Macrocyclic Peptides For Inhibition of Autophagy
… Scanning Unnatural Protease Resistant (SUPR) mRNA display to develop macrocyclic peptides targeting the autophagy protein LC3. The resulting peptides bound LC3A and LC3B—two essential components of the autophagosome maturation machinery—with mid-nanomolar affinities and disrupted …
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Characterization and evolution of artificial RNA ligases
… protein scaffold. The selection employed mRNA display, a technique to isolate de novo enzymes in vitro from large libraries of 1013 protein variants. The artificial RNA ligase catalyzes the formation of a phosphodiester bond between two RNA substrates by joining a 5'-triphospate to a …
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Reactive Peptides for Site-Selective Cysteine and Lysine Bioconjugation
… properties you are screening for. We turn to mRNA display, an in vitro selection technique that enables the screening of trillions of peptide sequences for desired functions. We show our efforts into establishing an mRNA display platform in our laboratory to discover novel binders and reactive …
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Targeting PfUCHL3 Using Chemically Constrained Peptides
… selected for against PfUCHL3. The peptides all display tight dissociation constants for PfUCHL3 in the low nanomolar range (6-35 nM) and 4 of the 10 peptides inhibit the activity of PfUCHL3 in cleaving its ubiquitin substrate *in vitro*. The peptides all have selectivity for PfUCHL3 over …
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Selection of a Non-Phosphorylated Peptide Inhibitor of BRCA1’s (BRCT)2 Domain
… its pharmacologic challenges. We generated an mRNA display library with 1.3 x 10^13 cyclized peptides covalently attached to the mRNA that encoded them. Eight rounds of selection exposing the library to a GST-BRCT fusion resulted in selection of non-phosphorylated peptides that bind to a BRCT …
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Long peptides for cysteine arylation
… small molecule, we performed an mRNA display selection. This uncovered a host of putative reactive peptides with little similarity at the sequence level. The primary isolate (MP01) displayed reactivity confirming the success of the selection and was sensitive to truncation and …