Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"mRNA destabilization"”.
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A Nascent Peptide Code for Translational Control of mRNA Stability in Human Cells
Stability of eukaryotic mRNAs is associated with their codon, amino acid, and GC content. Yet, coding sequence motifs that predictably alter mRNA stability in human cells remain poorly defined. Here, we develop a massively parallel assay to measure mRNA effects of thousands of synthetic and …
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A study of mammalian microRNA-mediated repression of gene expression by ribosome profiling
… gene expression by base pairing to their target mRNAs to direct repression. In animals, this repression is usually mediated through translational repression and/or mRNA destabilization. In studies that investigate miRNA-mediated repression with reporter constructs or individual endogenous genes, …
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Messenger RNA Destabilization by -1 Programmed Ribosomal Frameshifting
… genomic sequences, and suggested a role in mRNA stability. This work improves and extends the computational methods used to search for potential -1 PRF signals. It continues to examine four yeast -1 PRF signals and show that they promote significant mRNA destabilization through the nonsense …
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Restoring and enhancing Argonaute2-catalyzed cleavage
… either through Argonaute2-mediated slicing or mRNA destabilization and decay. The RNAi pathway provides both innate immunity against foreign sequences, such as viruses and transposons, and has been harnessed as an efficient gene-knockdown tool in many eukaryotic species, but curiously, not in …
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Destabilization of IL-8 mRNA by Anthrax Lethal Toxin: Demonstration of the Requirement for TTP and Examination of its Cellular Interactions
Control of mRNA stability is an important aspect in the regulation of gene expression. A well studied signal for rapid transcript decay in mammalian cells is the AU-rich element (ARE), which is found in the 3’ untranslated region (UTR) of many labile transcripts. These sequence elements confer …
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Function and regulation of poly(A)-tail length
… 3' ends of nearly all eukaryotic messenger RNAs (mRNAs) and long non-coding RNAs. The presence of a poly(A) tail promotes translation and inhibits decay of an mRNA, with both effects mediated through poly(A)-binding protein. However, an understanding of the relationship between the length of a …
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High-throughput sequencing of RNA 5'- and 3'-termini yields insights into viral and vertebrate gene expression
… vertebrate transcripts. The 5' ends of influenza mRNAs include heterogeneous sequences derived from host RNAs. In a process termed cap snatching, the viral polymerase cleaves host RNAs ~10-13 nucleotides downstream of their caps and uses the resulting fragments to prime viral transcription. …
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MicroRNA targeting in mus musculus and Caenorhabditis elegans
… to complementary sites in the target mRNA. The first miRNA, lin-4, was discovered in 1993 in Caenorhabditis elegans; since then hundreds of miRNAs have been identified in C. elegans, Drosophila melanogaster, plants, mouse, and humans, where they approach a number equivalent to 1-2% of …