Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 46 for “"mRNA degradation"”.
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Theory of mRNA degradation
… concerns the regulation of the stability of mRNAs. All organisms have evolved diverse and powerful mechanisms to achieve this. In order to better comprehend the regulation in living cells, biochemists have studied specific degradation mechanisms in detail. In addition to that, modern …
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Functions of Dcp2 and Ski7 In Mrna Degradation
… fidelity. This is partially achieved by mRNA decay. When no longer required, mRNA is degraded by two alternative pathways. The decapping enzyme Dcp2 removes the 5` <sup>m7</sup>G cap of mRNAs, allowing Xrn1 to degrade the mRNA from the 5` end. Alternatively, mRNA is degraded from the 3` …
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The Roles of Rnt1 and Putative Endoribonucleases in Eukaryotic mRNA Degradation
<p>Endoribonucleases initiate degradation by cleaving RNAs internally. Mutations in these enzymes have been shown to cause diseases such as cancer, developmental disorders, and neurodegenerative diseases. Yet, eukaryotic endoribonucleases have not been well studied, especially with regard to their …
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Deciphering the mechanism of Transferrin Receptor mRNA degradation under iron replete conditions
… regulated by changes in the stability of TFRC-1 mRNA. Under iron deplete conditions, iron regulatory proteins 1 and 2 (IRP-1 & 2) bind to specific sequence elements within the 3’ untranslated region (3’UTR) of the TFRC-1 mRNA and prevent its degradation. Whereas under iron replete conditions …
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Analysis of the role of poly(A) -binding protein (PAB1) in the mRNA degradation process in yeast
<p>The mRNA deadenylation process influences multiple aspects of protein synthesis and is known to be the major factor controlling mRNA decay rates. My data demonstrates that yeast PAB1 plays both positive and negative roles in controlling deadenylation, and I have identified particular regions of …
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Transcriptional elongation defects enhance upstream poly (A) site utilization and SPT5 affects mRNA degradation through its physical and functional interaction with CCR4 -NOT complex
… internal poly (A) sites, whose full-length mRNA formation is reduced by mutations in RNA polymerase II subunit RPB2, elongation factor SPT5, or TFIIS. RPB2 and SPT5 defects also promoted the utilization of upstream poly (A) sites for genes that contain multiple 3 ' poly (A) signaling …
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Developing Advanced Representation Learning Techniques for mRNA Sequence and Structure Modeling
… these sequences is essential for predicting mRNA degradation, a key factor in designing effective RNA-based therapies. However, many existing models struggle to capture the intricate relationships between sequence and structure, limiting their predictive power. We introduce StructmRNA, a …
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Identification of suppressors of spt10 that affect ADH2 expression in the yeast Saccharomyces cerevisiae
… subsequent to transcriptional initiation, namely mRNA degradation. Along with the recent identification of CCR4 and CAF1 as key components of the major yeast deadenylase, this research shows that there are important regulatory factors at multiple points of mRNA degradation which are important for …
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Translational regulation by short RNAs in mammalian cells
… major generalized roles in the regulation of mRNAs which are typically 100 times larger in size. These short RNAs, siRNAs and microRNAs, exert their influence on gene expression in mammals by RNA interference (RNAi) and translational repression. RNAi initiated by exogenous siRNAs results in …
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Computational analysis of small RNAs and the RNA degradome with application to plant water stress
… gene expression by targeting messenger RNAs (mRNAs) and directing endonucleolytic cleavage resulting in mRNA degradation. A snapshot of an mRNA degradation profile (degradome) can be captured through a new high-throughput technique called Parallel Analysis of RNA Ends (PARE) by using next …
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Messenger Rna Profiling: A Prototype Method For Body Fluidand Tissue Identification
… the presence and relative abundance of specific mRNA species. If the type and abundance of mRNAs can be determined in a stain or tissue sample recovered at the crime scene, it would be possible to definitively identify the tissue or body fluid in question. Advantages of an mRNA-based approach, …
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Regulation of the Mannose PTS Operon by the small RNA SgrS
… SgrS has been shown to stimulate degradation of the ptsG mRNA, encoding the major glucose transporter. This study demonstrates that SgrS regulates the genes encoding the mannose and secondary glucose transporter, manXYZ. Analysis of manXYZ mRNA stability and translation in the …
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Regulatory consequences of mRNA Poly(A)-Tail length changes
Eukaryotic mRNAs have a cap structure at their 5' ends and a poly(A) tail at their 3' ends, and the proteins that bind these features increase the stability and translation of an mRNA. The influence of the poly(A) tail on translation was discovered decades ago, but primarily with regard to the idea …
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The synthesis and in vitro evaluation of short interfering RNA containing triazole functionalized sphingolipid-based derivatives
… a sequence-specific manner by mediating targeted mRNA degradation. One of the main challenges in RNAi research is developing an effective delivery system for siRNAs. Recently, fatty acid conjugated siRNAs have shown promising potential as safe and effective method to deliver oligonucleotides to …
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Post-transcriptional control of gene expression
… at several levels, including transcription, mRNA degradation, translation and protein degradation. When intact, this system maintains cell homeostasis, keeping the cell alive and adaptable to different environments. Malfunction in the system can result in disease states and cell death. In …
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Alternative Mrna Splicing Redefines The Landscape Of Commonly Dysregulated Genes Across The Acute Myeloid Leukemia Patient Population.
… to this disease. Here, I investigate pre-mRNA splicing events with significant variation and striking coregulation across distinct AML cohorts. I find that most splicing events are expected to alter the expression of a subset of AML-associated genes independent of known somatic mutations. …
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The function of PAB1 in translation and in PUF3 dependent deadenylation
… of strains containing PAB1 variants, defects in mRNA degradation proteins, and defects in translation components. In chapter II, I address the role of PAB1 in regulated deadenylation. For this analysis I studied the effect of PUF3, a member of the PUF family of proteins that bind specific 3' UTR …
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Regulation of mRNA decay by Pumilio in Drosophila melanogaster
… development is driven by maternally loaded mRNAs synthesized in the female parent during oogenesis. These maternally synthesized mRNAs are regulated by post-transcriptional mechanisms, including mRNA localization, degradation and translational activation and repression. Many of these …
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Estrogen Regulates Vitellogenin Gene Transcription and Messenger-Rna Degradation in Xenopus Laevis Liver
Vitellogenin gene transcription and mRNA degradation rates were measured in Xenopus laevis liver using pulse labeling and pulse chase techniques. Estrogen regulates the accumulation of vitellogenin mRNA through three processes: (1) There is a selective transcriptional activation of the vitellogenin …
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The Influence of Osmolytes on Nucleic Acid Helical Conformations
… transcription, reverse transcription, and mRNA degradation. Our analyses revealed that the helical conformation of the hybrid duplexes ranged from A-form like to B-form like, depending on the base composition of each strand. In the presence of macromolecular crowding agents, the …
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