Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"mRNA cap"”.
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The mRNA Cap and Discontinuous Transcription in Trypansoma brucei
… phylogenetic order, Kinetoplastida, synthesize mRNA by a novel pathway that joins RNAs from physically unlinked gene segments. This results in the presence of a short non-coding RNA leader, called the miniexon, at the 5' end of most, probably all, mRNA. Indirect evidence suggests that …
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The regulation and function of mRNA cap methylation in pluripotency and differentiation
The synthesis of the N7-methylguanosine cap at the 5’ end of pre-mRNA occurs co-transcriptionally and is catalysed by a series of enzymes including the N7 RNA methyltransferase (RNMT), which along with its recently discovered activating subunit RAM, methylates the cap. RAM, which contains an RNA …
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REGULATION OF EUKARYOTIC GENE EXPRESSION BY mRNA CAP BINDING COMPLEX AND CAPPING MACHINERY
… the addition of a 5' terminal 7-methylguanine "cap" to nascent pre-messenger RNA (mRNA) in the nucleus. It is the 5'capping process, which proves vital to creating a mature mRNA. The synthesis of an mRNA followed by its capping is a complex undertaking which requires a set of protein factors. …
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Investigating the role and regulation of mRNA capping in pluripotency and differentiation
The mRNA cap added to the 5’ end of nascent transcripts is required for the efficient gene expression in eukaryotes. In vertebrates, the guanosine cap is methylated at N7 position by RNMT, which is in complex with its activating subunit RAM. Additionally, the first and second transcribed …
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Investigating the role of mRNA capping enzyme in C-MYC function
… transcription, C-MYC promotes formation of the mRNA cap which is important for transcript maturation and translation. However, the mechanistic details of C-MYC-dependent mRNA capping are not fully understood. Since anti-cancer strategies to directly target the C-MYC protein have had limited …
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Investigating the Role of the mRNA Capping Enzymes in Mouse Embryonic Stem Cells and Differentiation
The mRNA cap consists of a series of co-transcriptional modifications on nascent transcripts that confer protection from degradation and mediate RNA processing and translation. The addition of an inverted guanosine cap to the 5’-end of the transcript and its subsequent methylation along with the …
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Assessing eIF4E-S209 Signaling and Translationally Regulated Mechanisms in Neurodegeneration
… role in translational control through the mRNA cap binding protein, eukaryotic translation initiation factor 4E (eIF4E). Downstream of stimuli, ERK or p38 can phosphorylate the MAPK interacting serine/threonine kinases (MNKs) 1 and 2. MNK1 or 2 then phosphorylate eIF4E, at a specific …
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Single-Molecule Characterization of RNA via Nanopore Sensing
… that offer broad overviews typically struggle to capture sequence-specific features or detect chemical modifications which can profoundly influence cellular function and disease. Several RNA characterization techniques still rely on reverse transcription or amplification, introducing enzymatic …
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Pyp3 Involvement in Mitotic Control and Cell Growth in Fission Yeast
… proteins is tif1. eIF4E (Tif1) is the mRNA cap-binding protein and essential for recruitment of ribosomes to mRNA. Our results show that Pyp3 physically interacts with eIF4E in vivo and affects both the phosphorylation state and cellular distribution of eIF4E. In addition, …
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The Effects of Polysomal mRNA Association and Cap Methylation on Gene Expression in <i>Trypanosoma brucei</i>
… together are subsequently cleaved into separate mRNAs by trans-splicing and are individually regulated. During the process of trans-splicing, a 39-nucleotide splice-leader RNA is added to the 5´ end of mRNA. In this study, gene regulation in trypanosomes will be examined in the context of the …
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Development of chemical probes for intracellular nucleotide delivery, profiling of the metabolic fate(s) of nucleoside monoester phosphoramidates, and a nucleotide mimetic inhibitor of eIF4E
… substitute for 5´-phosphate in the design of 5´-mRNA cap analog antagonists of eIF4E. We successfully synthesized a mimetic of 7-Chlorophenoxyethyl guanosine monophosphate and demonstrated that its binding potency to eIF4E is comparable to that of the parent nucleotide, with only a modest loss in …
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Characterization of the Human Cytomegalovirus UL75 Late Gene Promoter
… Further deletion of sequences downstream of the mRNA cap site (+1) revealed the presence of upstream control elements that were masked by the dominant downstream activation domain. Mutational analysis demonstrated that a PEA3 element present in this downstream domain is important for promoter …
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The role of eukaryotic initiation factor 4E (eIF4E) in osteosarcoma metastasis
… translation the abundance and activation of the mRNA cap-binding phosphoprotein, eukaryotic initiation factor 4E (eIF4E) is considered to be both rate and process limiting. We describe for the first time, the biological role of eIF4E (new biology) in metastatic osteosarcoma. We employed a …
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Structural and functional characterization of proteins involved in the biogenesis of spliceosomal U snRNPs
… mit der UsnRNP Biogenese stehen bzw. das RNA 5 -cap binden. Dabei handelte es sich um die Dimethyltransferase TGS1 (Trimethylguanosin Synthase 1), die die zweifache Methylierung des 5 -Guanosins spezieller RNA-caps katalysiert. Der zweite Schwerpunkt lag auf der Charakterisierung eines …