Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"mRNA analysis"”.
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Effects of Long-Term Exposure of Normal C57BL/6J Inbred Mice to 17β-Estradiol on Gene Expression in Lymphocytes: mRNA Analysis of Lymphokines and bcl-2/fas
… on lymphokine and bcl-2/fas (proto-oncogenes) mRNA expression. We evaluated the effects of estrogen on the expression of genes for lymphokines, which are essential for the immune response. It is hypothesized that estrogen may regulate the immune system by modifying the expression of lymphokine …
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Transcriptional analysis of the role of CD8+ T lymphocytes in acute neural herpes simplex virus infection / David C. Tscharke.
… mediator, IFN-[gamma]. A non-directed method of mRNA analysis is applied to HSV infected ganglia with the specific aim of identifying transcripts that may be associated with CD8+ T cell activity in the nervous system.
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Cell-specific roles for CASK in the pathology of Optic Nerve Hypoplasia
… and deficits in vision-related tasks. Further analysis on a homozygous partial loss of function variant (Caskfl/fl) also displayed ONH with reduced numbers of RGCs. In order to understand the mechanisms underlying CASK-associated ONH, I explored whether RGCs, the projection neurons of the …
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Stability And Recovery Of Rna In Biological Stains
… that could be of use to forensic scientists. An mRNA based approach could allow the facile identification of the tissue components present in a body fluid stain and conceivably could supplant the battery of serological and biochemical tests currently employed in the forensic serology laboratory. …
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Characterization of protein secretion in Mycobacterium leprae using phoA fusions in Escherichia coli and Mycobacterium smegmatis
… were studied for gene expression and secretion. mRNA analysis identified transcripts for all Sec-dependent pathway proteins of 15 genes predicted to be secreted in M. leprae. PhoA fusion studies in E. coli showed that 5 of 6 (83%) ML proteins (ML0091, ML0097, ML0620, ML1811 and ML1812) were …
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The Role of Transforming Growth Factor-Beta In Alcohol-Induced Cardiac Fibrosis
… the use of histological techniques, mRNA analysis, apoptosis determination, and immunohistochemistry were employed to determine the functional and structural changes in the heart. Also, primary rat cardiac fibroblasts were cultured in the presence of 1 and 4mg/ml ethanol and assayed …
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ZFP36 proteins and mRNA targets in B cell malignancies
… (AREs) in 3’ untranslated (3’UTR) regions of mRNAs. The members of the human family, ZFP36L1, ZFP36L2 and ZFP36 are able to degrade mRNAs of important cell regulators that include cytokines, cell signalling proteins and transcriptional factors. This project investigated two proposed targets …
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Expression Analysis and Stem Cell Engineering
… called polonies, was applied to measure mRNA from several key stem cell genes in small numbers of ES cells. Due to its properties, we hypothesized that polonies would be uniquely poised to profile stem cells. Polonies were counted for Oct3 in a sample of 10 ES cells and from three …
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A study of the protective effects of an aspalathin enriched green rooibos extract on experimentally induced hepatic steatosis
… violet staining. The effect of GRT extract on mRNA relevant to lipogenic genes (PPAR-α, SREBP, FASN and CPT1), inflammation (TNF-α) and glucose metabolism (GCK and ChREBP) were assessed. Further investigation was done using leptin-receptor deficient C57BL6/J db/db mice (n=8/group) and their …
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pH Regulatory Molecules In The Tumour Microenvironment: Modulators Of Aggressiveness And Immune Profile Of Human Hepatocellular Carcinoma
… <br></br><br></br> <b>Results:</b> Our mRNA analysis showed that the expression of <i>CA9</i> was significantly correlated with the expression of the hypoxia-inducible factor 1α-related gene (<i>HIF1A</i>) and of the stem cell-associated markers CD24, CD133, EpCAM and KRT19. Moreover, …
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Role of androgens in testicular development and dysgenesis
… using Taqman RT‐PCR to determine the mRNA expression of key developmental genes after exposure to DBP. Sertoli cell number was reduced after exposure to treatments that reduced testicular testosterone levels, i.e. DBP alone or as a co‐treatment, TP and dexamethasone. Sertoli cell …
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Characterisation of CIS- and trans-acting factors that regulate the human alpha 2(1) procollagen gene
… to increased turnover of the a1(I) procollagen mRNA. Analysis of promoter activity showed that the promoter and first intron, which has been reported to contain negative regulatory elements, did not harbour any Fas-responsive elements. The -343 bp and the -2300 bp promoter constructs were …