Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 61 for “"mESCs"”.
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A study into the biology of mESCs exiting naïve pluripotency and technology development for subcellular proteomics
… mouse embryo. In recent years, the exit of mESCs from naive pluripotency has been partially characterised, and several protocols have been developed that can generate either transient or stable cellular states which are intermediate to mESC and EpiSC pluripotency, such as Epiblast-Like Cells …
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Attenuated Responses To Inflammatory Cytokines In Mouse Embryonic Stem Cells: Biological Implications And The Molecular Basis
… We have differentiated mouse ESCs (mESCs) to fibroblast-like cells (mESC-FBs) which were proved to have partially developed innate immunity. Using these cells as a model for comparison with mESCs, the insensitivity of mESCs to the cytotoxic effects from IFNg, which is an inflammatory …
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System to study colony-colony interactions in embryonic stem cells
… autocrine factors exist in murine ESCs (mESCs) and can influence their self-renewal and proliferation, predominantly in deficiency conditions. These observations were investigated based on a comparison of different groups of mESCs that were plated at varying cell densities. In this study, …
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Control of cell fate decisions of embryonic stem cells by mechanical forces
… here examines how mouse embryonic stem cells (mESCs) respond to externally applied forces and underlying substrates. We examined if mESCs can be directed to differentiate by external local forces through integrin mediated pathway. Surprisingly, we found that cyclic loading of the same stress …
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Effects of Trimethylamine N-Oxide on Mouse Embryonic Stem Cell Properties
… impact of TMAO on mouse embryonic stem cells (mESCs), which serve as an in vitro model of the early embryo and of other potent stem cell types. Briefly, mESCs were cultured in the absence (0mM) or presence of TMAO under two different sets of treatment conditions: long-term (21 days), low-dose …
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CRISPR activation screening at single-cell transcriptomic resolution: discovering new regulators of zygotic genome activation
… I test CRISPRa in mouse embryonic stem cells (mESCs) using two well characterised markers of ZGA as targets and, subsequently, I develop and validate a method for detecting short guide RNA (sgRNA) molecules in single-cell RNA sequencing libraries. In chapter 4, I select 230 screen candidates …
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Comparative Analysis of Antiviral Responses in Mouse Embryonic Stem Cells and in Mesenchymal Stem Cells
… are expressed at lower levels in mouse ESCs (mESCs) than in C3H10Tl/2 cells. However, mESCs are susceptible to LACV infection resulting in cell death, which can be reduced by IFNP pretreatment. Furthermore, IFNP induced expression of ISG 15, PKR and dsRNA receptor genes that play key roles in …
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Investigating the Role of the mRNA Capping Enzymes in Mouse Embryonic Stem Cells and Differentiation
… and RAM is particularly highly expressed in mESCs. RAM is essential for pluripotency maintenance by controlling the expression of pluripotency-associated transcription factors in a positive feedback loop manner. During neural differentiation, RAM is degraded in an ERK1/2-dependent …
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P120-Catenin Regulates Rest and Corest, and Modulates Mouse Embryonic Stem Cell Differentiation
… function. Using mouse embryonic stem cells (mESCs), mammalian cell lines, Xenopus embryos, and in vitro systems, we show that p120 directly binds to the zinc finger/DNA-binding region of REST, as well as to CoREST. Chromatin immuno-precipitation and other approaches indicate that p120 protein …
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Carbon Fiber Reinforced Lithium-Ion Battery Composites with Higher Mechanical Strength: Multifunctional Power Integration for Structural Applications
… of Multi-functional energy storage composites (MESCs) are examined. Experimental results on the electrochemical characterization reveal that the MESCs show comparable performance to the standard lithium-ion pouch cells without any external packaging and not under any loading requirements. The …
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Contribution of Lsh to DNA methylation reprogramming in embryonic stem cell, epiblast stem cell and embryoid body model systems
… to convert lsh-/- mouse embryonic stem cells (mESCs) to a hypomethylated ‘ground state’ of pluripotency. Culture conditions were then altered to transition these ground state mESCs to cells representing later, more methylated stages of development (‘serum’ mESCs, epiblast stem cells and …
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Investigating mechanisms of genome folding by single-cell Hi-C
… processed haploid mouse embryonic stem cells (mESCs) and calculated 3D structures of their entire genomes. The structures show several genome-wide features that are highly conserved across all mESCs, including: the genome shape is an ellipsoid; the chromosomes arrange in a Rabl configuration; …
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Getting at the Surface: A Promoter and Coding Sequence Characterization of an Odorant Receptor
… off and on marks in mouse embryonic stem cells (mESCs) to show that the M71 OR promoter, where M71 is thoroughly studied OR gene in ours and other labs, contains only off marks in mESCs. We established a high-throughput approach that scored for the expression of CRE protein and for the conversion …
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Investigation of the mechanisms underlying the effects of hyperglycaemia on cardiac structural and electrical remodelling
… Methods: Mouse embryonic stem cells (mESCs) were differentiated into pulsatile, cardiac-like cells via embryoid body (EB) formation and cultured under baseline- or high glucose conditions. A Ca2+ -sensitive fluorescent dye Fluo-4 was used to measure calcium transients and a …
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Investigating the role and regulation of mRNA capping in pluripotency and differentiation
… initiation. In mouse embryonic stem cells (mESCs), high levels of RAM maintain expression of pluripotency factors. Differentiation of mESCs to neural progenitors is accompanied by a suppression of RAM, resulting in downregulation of pluripotency factors and efficient formation of neural …
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Characterisation of HP1γ in mammalian cells
… for HP1γ in mouse embryonic stem cells (mESCs) and breast cancer cells. Mass spectrometry analysis identified citrullination of residues R38 and R39 within the CD of HP1γ. I show that these residues are citrullinated by peptidyl arginine deiminase 4 (PADI4) in vitro and in vivo. Mutations …
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Genetic Dissection of the Exit of Pluripotency in Mouse Embryonic Stem cells by CRISPR-based Screening
… by culturing mouse embryonic stem cells (mESCs) with MEK and GSK3 inhibitors (2i). The transcription network that maintains pluripotency has been extensively studied with the indispensable core factors being Oct4, Sox2 and Nanog, together with other ancillary factors reinforcing the …
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ROLE OF A SPECIFIC NANOG SUPER-ENHANCER ERNA IN EPIGENETIC REMODELING AND ENHANCER-PROMOTER INTERACTION
… the region 5kb upper the TSS of Nanog gene in mESCs has been investigated. In particular, a specific eRNA transcript, here defined as antisense eRNA, resulted actively involved in the maintenance of Nanog gene in an active epigenetic state. After the antisense eRNA silencing, Nanog was …
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Single molecule techniques to probe decision-making processes in developmental biology
… inner ear and single mouse embryonic stem cells (mESCs) during the process of X inactivation, to demonstrate our ability to perform single-cell, single-molecule assays that reveal both highly quantitative and spatial information. Accordingly, we adapted a high resolution, single-molecule RNA …
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Discovery of miRNA-regulated processes in mammalian development
… were performed in mouse embryonic stem cells (mESCs) and a miRNA-deficient derivative cell line, lacking miRNA-processing enzyme, Dicer, to enrich for targets of miR-290~295, a miRNA cluster highly-expressed in mESCs. By these methods, we have identified a set of candidate mRNAs that bear a …
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