Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"mESC"”.
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Gene Expression in mESC Deficient in FGFR Signalling and the Identification of the Novel Gene Ened
Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of blastocyst stage embryos, and upon differentiation into embryoid bodies (EBs) they recapitulate the genetic, cellular and morphological events occurring in early embryogenesis. Among several signalling pathways and inductive …
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HIGHLY SPECIFIC FLUORESCENT PROBE FOR MOUSE PLURIPOTENT STEM CELLS
… specific fluorescent probe was discovered for mESC via high throughput screening of compounds. The hit compound was named CDy9. High throughput screen found 23 hit compounds that are specific to mESC. The cell panel test identified CDy9 as the most specific compound. FACS sorting of CDy9 …
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Regulation of Dkk1, an Inhibitor of the Wnt Signaling Pathway, by DAX-1 in Mouse Embryonic Stem Cells
… of pluripotency in mouse embryonic stem cells (mESC), such that elimination of DAX-1 (through methods such as RNAi) leads to the differentiation of ESC (Khalfallah et al. 2009). Earlier research by the Tzagarakis-Foster lab identified target genes of DAX-1 in mESC through PCR array methodology …
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Effects of Trimethylamine N-Oxide on Mouse Embryonic Stem Cell Properties
… impact of TMAO on mouse embryonic stem cells (mESCs), which serve as an in vitro model of the early embryo and of other potent stem cell types. Briefly, mESCs were cultured in the absence (0mM) or presence of TMAO under two different sets of treatment conditions: long-term (21 days), low-dose …
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ZSCAN4 AND REPLICATION STRESS: SAFEGUARDING GENOME STABILITY IN MOUSE EMBRYONIC STEM CELLS
… that exposure of mouse embryonic stem cells (mESC) to RS induces the expression of Zscan4, a marker of the 2C stage in mouse embryo development. Depletion of Zscan4 in mouse zygotes reduces the number of blastocysts formed, which eventually fail to grow and implant. ZSCAN4 also plays a crucial …
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Hyperosmotic Stress Enzyme Signaling Modulates Oct4, Nanog, And Rex1 Expression And Induces Prioritized Differentiation Of Murine Embryonic Stem Cells
… cell mass, murine</p> <p>embryonic stem cells (mESC). Hyperosmotic stress slowed mESC accumulation due to</p> <p>slowing of the cell cycle, not apoptosis. PI3K signaling was responsible for cell survival</p> <p>136</p> <p>under stressed conditions. Stress initially triggered mESC differentiation …
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Developmental Regulation of Prion Expression in Cattle and Mouse Embryonic Stem Cells
… Finally, we used a mouse embryonic stem cell (mESC) model to study the potential role of PrPC during neurogenesis. In adult tissues, intense expression of PrPC was detected in the central nervous system (CNS), thymus and testes, whereas the liver, striated muscle and female reproductive tissues …
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Transcription factor antagonism regulates heterogeneity in embryonic stem cell states
… heterogeneity in murine embryonic stem cell (mESC) states. We identify differentially active and transcribed enhancers (DATEs) across states. DATEs regulate differentially expressed genes and are distinguished by co-binding of Kruppel-like transcription factors Klf4 and Zfp281. In contrast to …
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System to study colony-colony interactions in embryonic stem cells
… autocrine factors exist in murine ESCs (mESCs) and can influence their self-renewal and proliferation, predominantly in deficiency conditions. These observations were investigated based on a comparison of different groups of mESCs that were plated at varying cell densities. In this study, …
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Analysis of Interrelationships Among NAD+, PARP1, ADP-Ribosylation, and Splicing in Murine Embryonic Stem Cells
… catalytic activity during the early stages of mESC differentiation (e.g., within 12 hours of LIF removal) leading me to query the regulation and outcome of PARP1-mediated ADP-ribosylation in mESCs. NAD+ is synthesized through three main pathways - De novo, Salvage, and Preiss-Handler - and is …
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MES-DERIVED CARDIOMYOCYTES AS CELLULAR MODEL TO STUDY CARDIAC DISEASES: ALTERATIONS IN STRN AND GNB5 GENES
… and isogenic WT mouse embryonic stem cell (mESC) lines. 10-12-day old beating mESC-CMs were analyzed by Patch-clamp, motion video tracking, Ca2+ dynamics and immunofluorescence analysis. STRN-KO cells have a higher spontaneous beating rate and faster action potential depolarization (dV/dt) …
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Attenuated Responses To Inflammatory Cytokines In Mouse Embryonic Stem Cells: Biological Implications And The Molecular Basis
… We have differentiated mouse ESCs (mESCs) to fibroblast-like cells (mESC-FBs) which were proved to have partially developed innate immunity. Using these cells as a model for comparison with mESCs, the insensitivity of mESCs to the cytotoxic effects from IFNg, which is an inflammatory …
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Microfluidic perfusion culture for controlling the stem cell microenvironment
… for modulating diffusible cell-cell signaling in mESC culture, thus enabling more control over the soluble microenvironment over time. Non-recirculating microfluidic perfusion culture can effect a more defined microenvironment by continuously controlling the supply and removal of soluble factors, …
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An exploration of the molecular mechanisms underlying the effects of hyperglycaemia on the autorrhythmicity of cardiac-like stem cells
… autorrhythmicity of mouse embryonic stem cell (mESC)-derived cardiac-like cells.
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Cyclic loading of fibronectin coated beads induces differentiation in mouse embryonic stem cells
… directs mouse embryonic stem cell (mESC) fate. A magnetic twisting cytometer (MTC) was used to apply oscillating force to mES cells through fibronectin (FN) coated magnetic microbeads. The ligand coated magnetic beads were allowed to bind to undifferentiated mES cells plated on 0.6 …
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A study into the biology of mESCs exiting naïve pluripotency and technology development for subcellular proteomics
… mouse embryo. In recent years, the exit of mESCs from naive pluripotency has been partially characterised, and several protocols have been developed that can generate either transient or stable cellular states which are intermediate to mESC and EpiSC pluripotency, such as Epiblast-Like Cells …
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Transcriptional regulators in stem cell biology
… that BMI1 is required for melanocyte stem cell (MeSC) maintenance and function. RNAsequencing of MeSCs established that BMI1 loss leads to derepression of Cdkn2a and developmental transcription factor genes. Additionally, we observe downregulation of glutathione S-transferases, suggesting that …
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The cell-secreted microenvironment : shaping embryonic stem cell self-renewal and differentiation
… work showing that mouse embryonic stem cell (mESC)-secreted signals are required to maintain self-renewal, as cells enter a primed, epiblast-like state of early differentiation when microfluidic perfusion is used to deplete soluble cell-secreted signals. We show that this phenotypic change can …
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Carbon Fiber Reinforced Lithium-Ion Battery Composites with Higher Mechanical Strength: Multifunctional Power Integration for Structural Applications
… of Multi-functional energy storage composites (MESCs) are examined. Experimental results on the electrochemical characterization reveal that the MESCs show comparable performance to the standard lithium-ion pouch cells without any external packaging and not under any loading requirements. The …
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Control of cell fate decisions of embryonic stem cells by mechanical forces
… here examines how mouse embryonic stem cells (mESCs) respond to externally applied forces and underlying substrates. We examined if mESCs can be directed to differentiate by external local forces through integrin mediated pathway. Surprisingly, we found that cyclic loading of the same stress …
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