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Showing 1 to 20 of 29 for “"m6A"”.
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Investigation of m6A-regulated RNA-binding proteins
N6-methyladenosine (m6A) is one of the most ubiquitous modifications of eukaryotic RNAs. The modifi- cation functions to control the normal processing, export, splicing, and metabolism of cellular RNAs. All of these processes are mediated by a group of m6A RNA-binding proteins known as ‘readers’ …
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The role of the YTHDF m6A readers in neuroblastoma
… NB patients show that an overall increase in m6A-regulatory factors correlates with the worst prognosis. Notably, the m6A reader YTHDF1 is upregulated in stage 4 NB corresponding to the highest degree of MYCN amplification. In line with these notions, we observed a positive correlation between …
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M6A Reshapes the Folding and Recognition Landscape of RNAs
… effects on RNA metabolism. N6-Methyladenosine (m6A) is an abundant post-transcriptional RNA modification that influences multiple aspects of gene expression. While m6A is thought to mainly function by recruiting reader proteins to specific RNA sites, the modification can also reshape RNA-protein …
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ARCHITECTURE AND RNA RECOGNITION BY THE EUKARYOTIC M6A METHYLTRANSFERASE COMPLEX
… functions. Among them, N6-methyladenosine (m6A) is one of the most abundant mRNA modifications and contributes to key cellular processes, including mRNA processing, translation, and decay. In eukaryotes, m6A is installed by the m6A Methyltransferase Complex (MTC), a multi-protein assembly …
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Elucidating The Direct Role of M6A In The Human Transcriptome
… at position 6 to give N6-methyladenosine (m6A). m6A modification on cellular RNAs was discovered in the 1970s. The past decade has witnessed major progress that illustrated important roles of this mark in various aspects of gene control. Due to its pleiotropic roles, the direct effect of …
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Effects of m6A DNA methylation by bacterial methyltransferase in colorectal cancer
<p>Effects of m6A DNA methylation by bacterial methyltransferase in colorectal cancer Fabian Alejandro Mendoza Galvan Advisory Professor: Angela H. Ting, Ph.D. Fusobacterium nucleatum animalis (Fna) is found in the human oral cavity and gut. A distinct clade of Fna is primarily enriched in the …
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Role of N6-Methyladenosine (m6A) epitranscriptomic mechanisms in manganese-induced astrocyte inflammation
… this writing, namely that of N6-methyladenosine (m6A) epitranscriptomics and its regulatory tiers that control the writing, erasing, and reading of m6A modified RNAs. We find that the global m6A modifications are significantly affected in Mn exposed astrocytes. More specifically, our findings …
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THE EPITRANSCRIPTOMIC REGULATION OF STRESS: ROLE OF N6-METHYLADENOSINE IN ABIOTIC STRESS RESPONSE
… studies have shown that N6-methyladenosine(m6A), the most prevalent internal mRNA modification, can alter gene expression by affecting the stability, processing, and translation of mRNA among other functions. Very few studies have explored the involvement of m6A in the epitranscriptomic …
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Structures and Regulation of the N6-Methyladenosine Methyltransferase METTL16
… mRNA modification is N6 methyladenosine (m6A), which has been shown to regulate RNA splicing, translation and decay. The m6A modification is deposited by methyltransferases using S-adenosylmethionine (SAM) as the methyl donor. Determining the specificity and mechanism of action of m6A …
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CRISPR AS A TOOL FOR TARGET DISCOVERY AND TECHNOLOGICAL INNOVATION: HIGH-THROUGHPUT CBE SCREENING TO EVALUATE M6A MODIFICATION PATHWAY IN CANCER PROLIFERATION AND DRUG RESISTANCE, AND INNOVATIVE PRIME EDITING STRATEGIES FOR DISEASE MODELLING AND THERAPY
N6-methyladenosine (m6A) is the most abundant internal RNA modification of mRNA in mammals. The m6A -pathway comprises numerous effectors, which are classified based on their function. m6A is deposited co-transcriptionally by “writers”, including methyltransferase-like 3 (METTL3) and …
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Characterizing novel molecular regulators of antiviral gene expression
… is the RNA base modification N6-methyladenosine (m6A), which regulates many aspects of mRNA metabolism through transcript-specific effects. m6A deposition is mediated by a cellular complex of proteins including METTL3 and METTL14 (METTL3/14) and other cofactors, and m6A can also be removed from …
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Epitranscriptional Control of the Caspase-2—MDM2—p53 Axis: The Role of the m6A Writer in p53 Signaling.
… an unexpected role for N6-methyladenosine (m6A) RNA methylation as an essential post-transcriptional determinant of p53 activation in response to centrosome-derived stress. Using a genome-wide CRISPR/Cas9 screen coupled with a fluorescent reporter system, we uncovered all seven components of …
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INTERROGATING THE ROLE OF N6-METHYLADENOSINE RNA MODIFICATION ON TELOMERASE BIOLOGY
… has identified hTR to be an N6-methyladenosine (m6A) modified RNA. The presence of m6A adds another layer of complexity to hTR processing due to the ability of the mark to be added to specific RNA and act as a target for m6A selective RNA binding proteins. In the case of hTR, the addition of m6A …
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Role of m<sup>6</sup>A RNA "reader" YTHDF2 in Mammalian Germ Cells and Early Embryo Development
… in cell fate determination. N6-methyladenosine (m6A) is one of the most abundant and conserved internal mRNA modifications that plays important role in regulation of gene expression in development and disease. The m6A-RNA modification mostly destabilizes mRNA levels and its role is mediated by …
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Targeting Alternative Conformational States of the HIV-1 Rev Response Element Stem IIB using Small Molecules
… to be important for Rev binding. In addition, m6A modifications on HIV-1 RNA has shown their critical roles to HIV-1 replication, and two probably essential m6A sites on purine-rich region on RREIIB was discovered. However, the nature of the flexibility and m6A modification to RREIIB have …
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Posttranscriptional regulation with regard to cleavage factor I (CFI) associated alternative polyadenylation and methyltransferase A (MTA) assisted m6A modifications in Arabidopsis thaliana
… but not in Arabidopsis. N6-methyladenosine (m6A) is one of the most frequently observed RNA modifications, which is established by methyltransferase A (MTA) and other m6A writer proteins in Arabidopsis thaliana.<br /> This study revealed RNA binding sites of CFI subunit proteins GFP-CFI59 and …
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A Tale of RNA Circles: Function of Circular RNAs in Reproduction
… male germ cells; circRNAs tend to form between m6A-enriched sites on mRNAs, which are usually located around the start and stop codons. Consequently, ~46% of these spermiogenesis-enriched circRNAs contain translatable CDS with an m6A-modified start codon located within their junction sites. The …
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Uncovering the Roles of Human C2H2-Zinc Finger Protein ZNF121 in Gene Regulation
… gene regulatory mechanism. N6-methyladenosine (m6A) is the most abundant and well-characterized mRNA modification, facilitating mRNA regulatory processes that include mRNA decay regulated by the m6A reader proteins YTH-domain-containing family protein 2 (YTHDF2). However, YTHDF2 has a relatively …
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Genetic screens of RNA binding proteins identify a role for N6-methyladenosine in promoting B cell differentiation
… role of the RNA modification N6-methyladenosine (m6A) in B cells. This work demonstrated a role for the m6A binding protein Ythdf2 in promoting B cell terminal differentiation, independently of a role in proliferation or survival. In the future, I hope to validate an in vivo role for Ythdf2; and …
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Role of Methyltransferase Like-3 (Mettl3) In Pancreatic Ductal Adenocarcinoma (Pdac) Progression
… in the formation of N-6 methyl adenosine (m6A). m6A is the most abundant internal modification in mRNA and affects gene expression while also playing crucial roles in early-stage development, organogenesis, and cell-fate determination.</p> <p>To investigate the effect of METTL3 in PDAC, we …
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