Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"m5C"”.
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Genetic requirements and molecular functions of 5-methylcytosine in Caenorhabditis elegans RNA
… The methylation of carbon-5 of cytosines (m5C) in RNAs is a highly conserved and widely distributed feature in biological systems. Yet, the molecular functions of this modification, as well as the mechanisms through which this mark is interpreted are still poorly understood. In this thesis, …
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Evaluation of Computational and Experimental Parameters in RNA Bisulfite Sequencing Analysis and Applications in Brain Development Studies
… in a diverse range of RNAs. 5-methylcytosine, m5C, is an evolutionarily conserved and reversable modification that regulates the stability and export of tRNAs, rRNAs, and mRNAs. m5C has recently been implicated in many biological phenomena including tumorigenesis, embryonic cell expansion and …
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Investigating the role of RNA 5- methylcytosine on ribosomal and transfer RNAs in Arabidopsis thaliana
RNA 5-methylcytosine (m5C) is implicated to have multiple biological and molecular roles in single cell and multicellular organisms, including regulation of mRNA translation and plant growth. However, there are still many questions waiting to be answered. In my research, I addressed the roles of …
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Chemical and Enzymatic Methods to Detect Chemical Modifications in Nucleic Acids
… and tested to enable the chemical enrichment of m5C in RNA. A variety of enzymes characterised to modify genomic features are used for the detection of epigenetic features. One example is the use of Tn5 transposase in CUT&Tag. Chapter 5 describes the development of a method to covalently recruit …
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An Inverse Electron-Demand Diels-Alder Strategy for Mapping RNA Methylation
… 2'-O-methylation (Nm), and 5-methylcytosine (m5C), are critical regulators of gene expression. Mapping these modifications at single-nucleotide resolution, however, remains a major challenge as current methods often suffer from low resolution or antibody-related biases. While bioorthogonal …
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A systems-level analysis of dynamic reprogramming of RNA modifications in the translational control of cellular responses
… proteomics approach, it was demonstrated that m5C regulates the levels of the homologous ribosomal protein genes rp/22a and rp/22b, with hydrogen peroxide exposure causing an increase in the proportion of ribosomes containing rpI22a. Further, loss of rp/22a conferred sensitivity to hydrogen …