Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"lysosome fusion"”.
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Characterization of Phagosome-Lysosome Fusion in Macrophages
… were used to study the determinants of phagosome-lysosome (P-L) fusion in intact mouse macrophages. Lysosomes were labeled with the fluorescent vital dye acridine orange and the rate and extent of their fusion with yeast-containing phagosomes was monitored by fluorescence microscopy. Fusion was …
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The synthesis, characterization, and use of a protein-cysteine proteinase inhibitor complex for the study of endosome/lysosome fusion
The cysteine proteinases cathepsins B, L, and S are lysosomal enzymes responsible for the degradation of endocytosed proteins. Their presence in human cell monocytic lines THP1 and U937 was detected by the use of the membrane-permeable, irreversible, active-site directed inhibitor Fmoc-(¹²⁵I)Tyr- …
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The physiological role of P2X4 receptors in lysosome function
… to Ca2+. They are predominantly localised to lysosomes and from there can traffic to the cell surface. ATP levels within the lysosome are high but P2X4Rs are inhibited by the acidic pH. Previously, it was shown that the alkalinisation of lysosomes using pharmacological reagents was sufficient …
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Effects of in vivo dexamethasone sodium phosphate on in vitro equine neutrophil function
… Neither phagocytosis, iodination, or phagosome-lysosome fusion was affected by treatment. Hydrogen peroxide production, measured both spectrophotometrically and by flow cytometry, was not affected by treatment. Lysosomal enzyme activity for alkaline phosphatase, acid phosphatase, lysozyme, …
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Comprehensive definition of Ser/Thr/Tyr phosphorylation in mycobacteria: towards understanding reprogramming of normal macrophage function by pathogenic mycobacteria
… role in bacterial survival by blocking phagosome/lysosome fusion. We identified the in vivo physiological substrates of this kinase in actively growing M.bovis BCG culture. Our results revealed that this kinase is a regulator of protein synthesis. We then examined the mechanisms of survival in …
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Molecular and Cellular Mechanisms Responsible for Low-grade Stress and Inflammation Triggered By Super-low Dose Endotoxin
… the key regulators of mitochondrial fission and fusion, Drp1 and Mfn1 respectively, are inversely regulated. An increase in mitochondrial fragmentation and cell death which was not dependent on caspase activation was observed. In addition, super-low dose LPS was able to activate RIP3, a kinase …
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The Dynamics and Function of the Endolysosomal/Lysosomal System
Lysosomes are intracellular organelles that were considered for a long time to be simply an acidic and hydrolytically active end point of trafficking routes for degradation, in the last 20 years, light has been shed on their functional heterogeneity and striking role in signalling and nutrient …
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INVESTIGATING THE ROLE OF SNAP29 AND THE AUTOPHAGY PATHWAY IN DROSOPHILA MELANOGASTER MODELS OF C9ORF72 - LINKED ALS/FTD.
… (SNARE) protein involved in autophagosome-lysosome fusion, in modulating G4C2-induced toxicity. Through targeted downregulation of Snap29, we discovered that Snap29 promotes G4C2-induced toxicity in the fly eye. However, this effect was not recapitulated in motor neurons, despite the fact …
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Microbiology and cell biology of the interaction between Listeria monocytogenes and Acanthamoeba spp.
… (Cytochalasin D, Wortmannin), phagosome-lysosome fusion (Suramin) and phagosomal acidification (ammonium chloride, Bafilomycin A and Monensin). Sequence analysis of a section of the large plasmid from strain DRDC8 revealed a high level of similarity of gene organization and DNA sequences …
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Induction And Regulation Of Autophagy By Novel Prenylation Inhibitors In Sts-26t Malignant Peripheral Nerve Sheath Tumor (mpnst) Cells
… LAMP-2, which is required for autophagosome-lysosome fusion. These effects consequently lead to altered protein turnover and hence non-apoptotic cell death. Our observations identify a novel mechanism of action of GGTIs. We also show that autophagic cell death can be a consequence of an …
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Novel Mechanisms of Antigen Processing That Enhance Bcg Vaccine Efficacy
… of macrophages that do not fuse with lysosomes. Since lysosome fusion is necessary for peptide production and T cell priming leading to protective T<sub>H</sub>1 immunity, we hypothesized that vaccine efficacy is reduced and occurs perhaps due to non-lysosome dependent mechanisms. We …
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Role of Bacterial Effectors SopD and SopB in Pathogenicity of Salmonella enterica serovar Typhimurium.
… we have observed that SopB also inhibits fusion of lysosomes with Salmonella-containing vacuoles (SCVs) following invasion. This ability depends on SopB-mediated reduction of negative membrane charge of the SCV during invasion by hydrolysis of the phosphoinositide PI(4,5)P2. Membrane …
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Cationic amphiphilic drug-induced autophagosome accumulation is due to autophagosome sequestration within vimentin intermediate filament networks resulting in prolonged autophagosome half-life
… content did not inhibit</p> <p>autophagosome-lysosome fusion. Therefore, U18666A-mediated effects on</p> <p>trafficking were investigated.</p> <p>Fluorescent microscopy analysis revealed U18666A treatment affected</p> <p>F-actin and vimentin, but not microtubules. Rhodamine-phalloidin …
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The Membrane Polypeptides of the Vacuolar System: Composition and Recycling
… iodinating system into the confines of the phagolysosome, allowing us to study the nature of the phagolysosomal membrane. Lactoperoxidase (LPO) is covalently coupled to carboxylated latex spheres (LPO-latex) in a stable enzymatically active form. The addition of LPO-Iatex to cultured macrophages …
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Virulence and pathogenic mechanisms of <i>Legionella pneumophila</i>
… in tissue culture over 48 hours and phagosome lysosome fusion was not inhibited in these macrophages. Avirulent strains did not multiply but those produced by passage on supplemented Mueller Hinton agar (MHIH) maintained viability whereas those produced by passage on buffered charcoal yeast …