Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"lysosomal pH"”.
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Ionic Mechanism of Lysosomal Function and Cell Metabolism
<p>Two Pore Channels (TPCs) are endolysosomal ion channels that are permeable to sodium and calcium. Defects in TPCs have been implicated to impair vesicle trafficking, autophagy and cell metabolism control; however, the detailed mechanism remains largely unknown. In this study, I show that TPCs …
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Organelle remodeling and V-ATPase dynamics in the lysosome regeneration cycle
… our knowledge of the molecular biology and morphology of these organelles, revealing their diverse range of functions. The heterogeneity in lysosomal pH, catalytic activity, and localisation, within the lysosome regeneration cycle has been investigated in this work. This thesis provides …
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Liposome drug delivery systems for anticancer agents
<p>Development of liposome formulation of an amphiphilic anticancer peptide using the ANTS/DPX leakage assay. The effects of lipid composition on the liposomes' resistance to an amphiphilic cyclic peptide c[KS.S.S.KWL W] were studied by the ANTS/DPX leakage assay. One or more unsaturated acyl …
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The Effects of Four Consecutive Days of Acute Exercise on Macrophage Antigen Presentation
… the effects of exercise on the ability of M&phis;'s to present antigen to T cells. Pathogen-free male Balb/c mice (8 +/- 2 wk) were randomly assigned to either home cage control (HCC), moderate exercise (MOD, 18 m min-1, 5% grade, 0.5 hr day1), exhaustive exercise (EXH, 18--30 m min-1, 3 hr …
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The function of the hepatitis C virus p7 protein
… To evaluate the contribution of p7 to organelle pH regulation and virus production, a fluorescent pH sensor was incorporated within native, intracellular vesicles in the presence or absence of p7. p7 increased proton conductance in vesicles and was able to rapidly equilibrate H+ gradients. This …
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A study of the mechanism of action of novel inhibitors of tumour cell invasion
… identified: motuporamine C (MotC) and strongylophorine-26 (STP-26). Structure-activity studies identified dihdyromotuporamine C (dhMotC) as a potent and easily synthesized analogue. In this work, the mechanism of activity of dhMotC and STP-26 was investigated. It was found that both dhMotC and …
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THE LYSOSOMAL AMYLOID AGGREGATION OF B2-MICROGLOBULIN PROMOTES MULTIPLE MYELOMA PROGRESSION: A MOLECULAR FOCUS ON THIS PRO-CANCER MECHANISM
… inside the lysosomes of tumor-associated macrophages. The resulting lysosomal damage induces NLRP3 inflammasome activation which sustain inflammation and malignant plasma cell proliferation. This thesis investigates the link between β2m fold stability and aggregation propensity under …
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The effects of the Parkinson’s disease-associated proteins LRRK2 and TMEM175 on the lysosomal profile of cultured mammalian cells under lysosomal stress conditions
Lysosomal dysfunction is increasingly recognised as a central factor in the development of Parkinson’s disease (PD). At least 11 out of the 24 PD-associated genes have been identified by genome-wide association studies (GWAS) to be involved in autophagy-lysosome pathways. Leucine-rich repeat kinase …
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The Role of Compartmentalized Metabolism in Cellular Metal Homeostasis
… organelles, which accompanied the rise of atmospheric oxygen and an increased need for iron, also play a key role in iron homeostasis. Mitochondria are the sites of iron assimilation whereas lysosomes, with their v-ATPase-generated acidic lumens, are responsible for iron uptake and rely. In the …
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Characterisation of an immune-modulating peptide secreted by a helminth worm
… Th17 immune responses. This immune-modulatory phenomenon is attributable to the molecules excreted/secreted by helminths as they migrate through their human hosts. The identification of these molecules has the potential to contribute to the development of novel therapeutics for the treatment of …
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Characterization of Phagosome-Lysosome Fusion in Macrophages
… were used to study the determinants of phagosome-lysosome (P-L) fusion in intact mouse macrophages. Lysosomes were labeled with the fluorescent vital dye acridine orange and the rate and extent of their fusion with yeast-containing phagosomes was monitored by fluorescence microscopy. …