Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"lysosomal membrane"”.
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Biogenesis of lysosomes in macrophages : intracellular pathway of lysosomal membrane protein to lysosomes
Includes abstract. Includes bibliographical references (leaves 212-248).
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Studies of intraorganelle dynamics: the lysosome, the pre-lysosomal compartment, and the golgi apparatus
… as an example to study intraorganelle dynamics. Lysosomal contents and membrane proteins were shown to intermix rapidly in fused mammalian cells, with a t<sub>½</sub> of ~30 min. Lysosomal content intermixing, shown by a sensitive invertase-lysosome/[¹⁴C]-sucrose-lysosome pairing assay, was …
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Efficacy of cellular biomarkers in marine filter feeding organisms for detecting effects of anthropogenic pollution
… established a strong relationship between lysosomal membrane stability in oysters, reproductive effects and contaminant exposure associated with re-suspended sediments. In the Sydney rock oyster, the relationships between lysosomal membrane stability and contaminant exposure were consistent …
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Identification of novel proteins that regulate the amino acid-sensitive mTORC1 pathway
… of mTORC1 from the cytosol to the surface of the lysosomal membrane, allowing it to be activated downstream of growth factors. This amino acid-induced translocation is mediated by the Rag GTPases and Ragulator (the scaffold for the Rag GTPases and mTORC1 on the lysosomal membrane). Here we …
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Lysosomal nutrients and the mTORC1 pathway
… cellular homeostasis, a better understanding of lysosomal function and metabolism and its relation to the mTOR pathway is necessary. Most components of the nutrient-sensing machinery upstream of mTORC1 localize to the lysosomal surface, and amino acids generated by lysosomes regulate mTORC1 by …
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Plasma membrane-derived vesicles: composition and function
Plasma membrane-derived vesicles (PMVs) are small, intact, membrane vesicles, which are released by most cells upon activation with various extracellular stimuli. This study identified that procedures used in the isolation of PMVs could be further improved by first sonicating (sonicating …
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The Liver as a Target Organ for the Non-Genomic Actions of Estrogen
… for the ligand, and isolate and identify a membrane protein which binds the ligand and free estradiol. I present results which identify the liver as a major tissue with a non-genomic response to estrogen, namely, the uptake and translocation of the ligand from a plasmalemmal and microsomal …
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Characterization of Palmitic Acid Induced Lipotoxicity in Schwann Cells
… GRP78. In SC cultured in euglycemic conditions, lysosomal membrane destabilization preceded mitochondrial membrane depolarization, oxidative stress and caspase 3/7 activation. The release of cathepsin L and B were also observed with PA treatments. However, in hyperglycemic SCs, PA-LTx induced- ER …
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Regulation of the mTORC1 growth pathway by amino acids
… by promoting the translocation of mTORC1 to the lysosomal surface, its site of activation. The Rags are unusual GTPases in that they function as obligate heterodimers, which consist of RagA or B bound to RagC or D. We show that RagC/D is a key regulator of the interaction of mTORC1 with the Rag …
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Structures and Mechanisms of Lysosomal Transporters
Lysosomal membrane transporters are indispensable for maintaining lysosomal homeostasis and proper function. Indeed, mutations in these key proteins can lead to debilitating disorders known as lysosomal storage diseases. Cystinosin and Sialin are two such transporters. Both proteins utilize the low …
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Intracellular trafficking of Leishmania major peptidases
… secretory signal peptides and potential transmembrane domains. The aim was to find a peptidase accumulating in the flagellar pocket of the cell, from where it could be secreted. Five candidate peptidases (a ubiquitin hydrolase, a CaaX prenyl protease, a zinc carboxypeptidase and two rhomboid …
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Morphological and histochemical observations of hepatic peroxisomes and lysosomes of rats after treatment with methylmercuric chloride, hydrogen peroxide and gamma radiation
… rats demonstrated disruption of peroxisomal, lysosomal and mitochondrial membranes and formation of different shapes and sizes of peroxisomes at day 2. Damage appeared to be mild at day 7 and no apparent damage persisted until day 21. Following the whole body irradiation of 750 R, granularity …
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Identification of Cathepsin B and L as Novel Uva Targets Upstream of Cutaneous Lysosomal-Autophagic Dysregulation
… inactivation upstream of autophagic-lysosomal dysfunction. In human skin fibroblasts, exposure to noncytotoxic doses of chronic UVA (9.9 J/cm ², twice a week, 3 weeks) caused pronounced photooxidative impairment of cathepsin B and L enzymatic activity suppressed by antioxidant …
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Organelle remodeling and V-ATPase dynamics in the lysosome regeneration cycle
… diverse range of functions. The heterogeneity in lysosomal pH, catalytic activity, and localisation, within the lysosome regeneration cycle has been investigated in this work. This thesis provides evidence that during organelle remodelling in the lysosome regeneration cycle, lysosomal pH is …
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Using human induced pluripotent stem cells to model cystinosis
Cystinosis is a rare lysosomal storage disease caused by mutations in the CYSTINOSIN (CTNS) gene, encoding a cystine transporter located on the lysosomal membrane. Cystinosis leads to an accumulation of cystine within lysosomes in most cells of the body, however the kidney is the main organ …
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Differential Autophagic Responses to Nano-Sized Materials
… titanium dioxide nanoparticles, however, induced lysosomal membrane permeabilization, leading to blockage of autophagic flux and accumulation of autophagic substrates. These results point to the complexity of the autophagic response to nanomaterials, which may involve activation of the …
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Structure-function studies of SLC15 peptide transporters
… large extracellular domain (ECD) between transmembrane (TMD) 9 and 10. Human PepT1 (hPepT1) and hPepT2 mutants with their ECD deleted (ECDΔ) or swapped (hPepT1-ECD2, hPepT2-ECD1) were tested for functional effects. The hPepT1-ECDΔ and hPepT1-ECD2 transporters were found not to express, showing …
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Proteomic analysis of the sorting machineries involved in vesicular traffic between the biosynthetic and endosomal compartments
… virus (Alconada et al., 1996), while sorting of lysosomal membrane proteins like Lamp-1 and LimpII is AP-3 regulated (Eskelinen et al., 2003). To study how AP-1A and AP-3 coats are stabilized on membranes and to identify the protein networks involved, a liposome based in vitro assay that …
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The Hydrophobic Handoff Between NPC2 and the N-Terminal Domain of NPC1 in the Export of Cholesterol from Lysosomes
… of 132 amino acids; and NPC1, an intrinsic membrane protein of 1278 amino acids and 13 postulated membrane-spanning helices that span the lysosomal membrane. Recessive loss-of-function mutations in either NPC2 or NPC1 produce NPC disease, which causes death in childhood owing to cholesterol …
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The role of lysosomes in radiation induced genomic instability
… This study was designed to investigate lysosomal involvement in radiation induced NTE, whether it be downstream of one of the above mentioned mechanisms, or independent of their involve- ment. To this end the primary human fibroblast cell line, HF19, was exposed to X-rays at therapeutic …
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