Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 55 for “"lysosomal degradation"”.
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With No Lysine 1 (WNK1): A Potential Regulator of The Lysosomal Degradation Pathway
… an aberrant endosomal structure, slowing protein degradation via the lysosomal pathway. I hypothesize that WNK1 delivers cargo to VPS4a to facilitate the degradation of plasma membrane proteins.
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Ionic Mechanism of Lysosomal Function and Cell Metabolism
<p>Two Pore Channels (TPCs) are endolysosomal ion channels that are permeable to sodium and calcium. Defects in TPCs have been implicated to impair vesicle trafficking, autophagy and cell metabolism control; however, the detailed mechanism remains largely unknown. In this study, I show that TPCs …
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Investigating The Role Of Chaperone-Mediated Autophagy In Glioma Stem Cells
… selectively targets cytoplasmic proteins for lysosomal degradation, is significantly upregulated. Moreover, increasing of CMA activity through either forced expression of LAMP2A or the CMA activator QX77 leads to GSCs differentiation as well as reduced self-renewal capacity, whereas knockdown …
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V-ATPase regulation of Hypoxia Inducible transcription Factors
… are inactivated by ubiquitin-proteasome-mediated degradation of their a subunit, which is dependent on prolyl hydroxylation by 2-oxoglutarate (2-OG) and Fe(II)-dependent prolyl hydroxylases (PHDs). In hypoxia, HIF-$\alpha$ is no longer hydroxylated and is therefore stabilised, activating a global …
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The Role of Autophagy in Early Development and Tumor Suppression Using a Zebrafish Model System
Autophagy is an evolutionarily conserved lysosomal degradation pathway which involves the sequestration of cytoplasmic components into a double membraned structure called the autophagosome. By using genetically manipulated autophagy-deficient models, important roles for autophagy in development and …
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Interactions between Human Two-pore Channels and Nonaspanin Proteins
Autophagy is an evolutionary conserved lysosomal degradation pathway which is involved with a variety of cellular process, but the relevant mechanisms remain elusive. Here I find that TM9SF1, a nine-spanning transmembrane protein and human two-pore channels (TPCs) regulate cell autophagy. More …
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Clinical importance of autophagy dependency and inhibition in cancer treatment
Autophagy, a lysosomal degradation recycling process, has a complex and context-dependent role in cancer. Certain cancers have been found to be inherently dependent on autophagy for survival regardless of the environment. Autophagy is also implicated as a mechanism of resistance to many …
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Interplay between Cannabinoids, SOCS3, and Autophagy Process in the Intestinal Epithelium with the Use of an Established in vitro Model System.
… and regulated cellular protein recycling and degradation via the lysosomal degradation pathway. Emerging data associates Crohn's Disease (CD) with an impaired ATG16L1 autophagy gene. Increased activity in the endocannabinoid system and up-regulation of suppressor of cytokine signalling …
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Molecular Mechanisms Regulating Chemokine Receptor CXCR4 Signaling and Trafficking
… onto early endosomes and targeted for lysosomal degradation, giving rise to long-term attenuation of signaling. The ubiquitin moiety on CXCR4 serves as a sorting signal for entry into the endosomal sorting complex required for transport (ESCRT) pathway. This pathway consists of four …
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Ube4B Levels Determine The Efficacy of Egfr and Stat5 Inhibitors In Treatment Resistant Neuroblastoma
… ligase, ubiquitinates the EGFR and promotes its lysosomal degradation ultimately attenuating EGFR signaling. Interestingly, the UBE4B gene lies in a chromosomal region (1p36) whose loss is correlated with poor patient outcomes due to inefficient EGFR degradation and enhanced cell proliferation. …
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Characterising Futile Autophagosome Based Toxicity and its Implications in Disease
… which then shuttle to lysosomes for degradation. Autophagy is implicated in numerous diseases, largely in a pro-survival role. However, autophagy has also been suggested as a form of programmed cell death (PCD), from cases of dying cells showing autophagosome accumulations. Debate …
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Structural characterization of interaction of K11 and K63 mixed-linkage polyubiquitin chains with the tUIMs of epsin1
… endocytosis as well as endosomal sorting for lysosomal degradation. Epsin1 is an endocytic adaptor protein with three tandem UIMs (Ubiquitin Interacting Motifs) which are responsible for the highly specific interaction between epsin and ubiquitylated receptors. Epsin1 is also an oncogenic …
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Binding properties of adaptor proteins Tollip and Tom1
… with endosomes is proposed to be involved in the lysosomal degradation of polyubiquitinated cargo. Through cellular, biochemical, and biophysical techniques, we have further characterized the association of Tom1 with Tollip. Mutations in the binding interface of the Tom1 GAT and Tollip TBD complex …
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Notch Regulates Vascular Collagen IV Basement Membrane Through Modulation of Lysyl Hydroxylase 3 Trafficking
… of LH3 from Col IV-containing vesicles and rapid lysosomal degradation of Col IV. Furthermore, we demonstrate that Rab10 is Notch responsive, indicating a novel connection between permissive Notch-based blood vessel maturation programs and vesicle trafficking. Our results illustrate both a new …
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Investigation of barriers to non-viral gene delivery and design of novel polymer-based gene delivery systems
… for investigating both the cellular uptake and lysosomal trafficking of non-viral vectors, two important barriers to efficient gene transfer. Using this and other tools we investigated the gene transfer properties of polyethylenimine (PEI), a highly effective non-viral vector material. We …
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Role And Regulation Of ESCRT-III In Multivesiculr Body Biogenesis
… ESCRT-III and VPS4 to undergo downregulation via lysosomal degradation. Taken together the studies in this thesis provide insights into roles and regulation of ESCRT-III in MVB biogenesis.
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Characterization of PCSK9-mediated LDLR Degradation in Hepatic and Fibroblast Cells
… subtilisin/kexin type 9 (PCSK9) mediates degradation of low-density lipoprotein receptors (LDLR) indicates a critical role in LDL metabolism. PCSK9 is a secreted protein that binds to the epidermal growth factor-like (EGF)-A domain of LDLR and directs the receptor for degradation in …
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Pathways of intracellular protein degradation in cultured muscle cells
… to elucidate the relative contributions of lysosomal and non-lysosomal degradation pathways functioning under varying nutritional states and for different classes of intracellular proteins. Proteolysis in cultured bovine aortic smooth muscle cells was measured as the percentage of …
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Structural and Functional Studies of the Human Saposin Proteins
… activator proteins that are essential for the lysosomal degradation of sphingolipids (SLs) with small headgroups. They function in part to increase the solvent accessibility of these SLs to specific acid hydrolases by two proposed modes of action. In the solubilizer model, saposins extract SLs …
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