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Showing 1 to 10 of 10 for “"lux operon"”.
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Role of region 4 of the sigma 70 subunit of RNA polymerase in transcriptional activation of the lux operon during quorum sensing
… dependent activation of the luminescence (lux) genes of V. fischeri relies on the formation of a complex between the autoinducer molecule, N-(3-oxohexanoyl) homoserine lactone, and the autoinducer-dependent transcriptional activator LuxR. LuxR, a 250 amino acid polypeptide, binds to a site …
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Transcriptional Control during Quorum Sensing by LuxR and LuxR Homologues
… of Vibrio fischeri activates the luminescence (lux) operon when the autoinducer signaling molecule (N-3-oxohexanoyl homoserine lactone) is recognized and bound by the activator protein LuxR. LuxR subsequently binds to the lux box centered at à 42.5 bp upstream of the transcription initiation …
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CHARACTERIZING THE ROLE OF PUTATIVE VIRULENCE GENES ASSOCIATED WITH INFECTION, COLONIZATION AND PERSISTENCE OF SALMONELLA ENTERITIDIS IN CHICKEN USING A BIOLUMINESCENT REPORTER
… (490nm) using the light-emitting module called lux operon. This technique has been widely used in various bacteria enabling live imaging in mouse models. We were able to accurately define the onset of primary colonization as early as 30 hours post-infection (p.i.) in the cecum with the aid of …
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Visualisation of bioluminescent Vibrio midae SY9 K811 in juvenile Haliotis midae abalone using non-invasive bioluminescence imagining
… labelled with a bioluminescent plasmid (pKluxCat) containing the lux operon from Photorhabdus luminescens. The plasmid was modified by the addition of the Tn9 chloramphenicol acetyltransferase (cat) gene to enable selection of Vibrio transconjugants. The bacterial cells were labelled using …
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Analysis of the Regulons Controlled by Transcriptional Regulators LuxR and LitR in Vibrio fischeri
… analysis previously identified several non-Lux proteins in V. fischeri MJ-100 whose expression was dependent on LuxR and 3-oxo-hexanoyl-L-homoserine lactone (3-oxo-C6-HSL). A lacZ reporter was used to show that the promoters for qsrP, acfA, and ribB were directly activated via …
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Elucidation of mechanisms of antibiotic subversion in mycobacteria
… inactivating systems, and the activity of efflux pumps. This study aimed to investigate the role of various components of the "intrinsic resistome" that limit the efficacy of antitubercular agents. The DNA damage response: the SOS response was hypothesized to play a role in antibiotic- …
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Multi-tiered Regulation of luxR Provides Precise Timing and Maintenance of the Quorum Sensing Response of Vibrio fischeri
… A key regulator of this system is LuxR, which is the transcriptional activator of the lux operon as well as several other genes in. LuxR also autoregulates its own transcription, which we have shown causes bistability and hysteresis in the quorum-sensing response. This behavior …
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Structure/Function Analysis of the Quorum-sensing Regulator EsaR from the Plant Pathogen Pantoea stewartii
… are key to the process of quorum sensing, the LuxI and LuxR homologues EsaI and EsaR. Most LuxR homologues function as activators of transcription in the presence of their cognate acylated homoserine lactone signal (AHL). EsaR utilizes an AHL-response opposite of the majority of the LuxR …
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Amino Acid Residues in LuxR Critical for its Mechanism of Transcriptional Activation during Quorum Sensing
… the N-terminal domain of a regulatory protein, LuxR. In response to autoinducer binding, LuxR is believed to undergo a conformational change that allows the C-terminal domain to activate transcription of the luminescence or <I>lux</I> operon. To further understand the mechanism of LuxR-dependent …