Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"lumen formation"”.
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Rab11 Regulates Lumen Formation and Morphogenesis in the Developing Pancreas
The molecular links between epithelial lumenogenesis and tissue-level morphogenesis in the pancreas remain elusive despite a decade of studies. Here, I show that deletion of Rab11A and Rab11B (Rab11) in the developing pancreatic epithelium (Rab11pancDKO) disrupts both of these processes. The loss …
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Mechanisms and dynamics of size regulation and lumen formation in mouse double embryos
… a process of epithelialization to form a lumen that later will become the pro-amniotic cavity, an essential morphogenetic event that is delayed in double embryos compared to normal-sized embryos. Mechanistic studies demonstrate that pro-amniotic cavity formation and size-regulation, albeit …
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Molecular control of endothelial lumen formation by Rho GTPases in three dimensional collagen matrices
… Rac1 play a key role in endothelial cell (EC) lumen and tube formation in 3D collagen matrices, and that their regulation is mediated by various downstream effectors including Pak2, Pak4, Par3, and Par6b. Both Pak2 and Pak4 phosphorylation strongly correlate with the lumen formation process in …
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Cell mechanics-based modelling and in toto imaging describe a new mode of lumen formation in chicken neural tube
Lumen formation creates epithelial tubes and vesicles that underlie the morphology and function of many major organs, such as ear, brain, and lung. The specific morphogenetic pathways taken may vary between organs and species, but the resultant single lumen remains constant. In chick neurulation, …
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The Roles of daf-6 and Cell-Cell Interactions in Sensory Organ Morphogenesis
… of neuronal sensory endings accommodated in a lumen formed by ensheathing epithelia or glia. The generation of this structure may require cell-autonomous factors that control lumen formation, as well as cell non-autonomous factors that coordinate the morphogenesis of the lumen with the resident …
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The role of Wnt signalling during embryonic salivary gland development
… alongside branching morphogenesis occurs lumen formation, which corresponds to the cavitation of the inside of the epithelial branches to form ducts. Branching and canalization take place in a controlled manner, so that branching is active at the distal ends of epithelial branches while …
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Bioartificial matrices to modulate epithelial morphogenesis
… proliferation, and promote polarization and lumen formation. However, the utility of natural BMs for detailed studies is generally limited by lot-to-lot variations, uncontrolled cell adhesive interactions, or growth factor contamination. The goal of this thesis was to engineer bioartificial …
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The role of cell division during tissue morphogenesis
… of cytokinesis and abscission during de novo lumen formation. The cytokinetic bridge places at the center of the developing KV rosette, where it acts as a landmark for Rab11-mediated vesicle trafficking to bring polarity components such as CFTR to the site of future lumen formation. Next, the …
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Blood Vessel Development
… function depends on patent blood vessel formation by endothelial cells. In this dissertation, a careful analysis of different aspects of blood vessel development was conducted in order to expand our understandings of the biology of blood vessel. In brief, Chapter 2 provides an in depth …
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Design of a microreactor for studying the effect of shear stress on angiogenesis in self-assembling peptide matrix
Understanding blood vessel formation has become a principal, yet challenging, objective of bioengineering over the last decade. Unraveling the complex mechanisms of angiogenesis could lead to the development of pro or anti-angiogenic treatments for diseases like heart disease and cancer, as well as …
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Modeling mammary epithelial cell polarization and the role of podocalyxin in breast tumor progression
… role of cell-cell and cell-ECM adhesion in the formation and polarization of the apical junction complexes in normal mammary epithelial cells. Cell-cell adhesion alone was sufficient to initiate polarized junction assembly. However, the addition of exogenous ECM generated a spatial polarity …
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Microfluidic-based 3D cell culture for studies of biophysical and biochemical regulation of endothelial function
… tip cell dynamics, cell division events and lumen formation. Of particular relevance to tissue engineering community, we demonstrated that endothelial cells when cultured for several days can assemble into vascular networks with open, perfusable lumen. Using this new system, we present novel …
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Dissecting Pluripotency and Mammalian Embryonic Development via Droplet Microfluidics
… conditions. Single cell RNA-seq and the formation of blastocyst chimaeras were then used to confirm the re-establishment of the complete naïve network. In contrast, plakoglobin is absent or greatly reduced during primed pluripotency in epiblast-derived stem cells and conventional primate …
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Regulation of Cell Adhesion By The Ferm Proteins, Ptpn14 and Merlin
… E-cadherin localization during primordial lumen formation. In cells with established AJs and TJs, decreased levels of PTPN14 led to an increase in β-catenin Y654 phosphorylation, promoting increased membrane mobility of E-cadherin and subsequent destabilization of cell contacts. This was …
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A Tissue-Engineered Microvascular System to Evaluate Vascular Progenitor Cells for Angiogenic Therapies
… necessary to support physiological microvessel formation in vitro; (2) poly(ethylene glycol) (PEG) hydrogel systems can support 3D microvessel formation by hCB-EPCs in coculture with mural cells; (3) mesenchymal cells, derived from either umbilical cord blood (MPCs) or bone marrow (MSCs), can …
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The Influence of Structure and Surface Biochemistry on Cell Function and Angiogenesis in Collagen Scaffolds
… features and surface chemistry properties on the formation of microvessels. Firstly, the influence of mould design on architectural features of collagen scaffolds fabricated using lyophilisation was assessed using micro-computed tomography (Micro-CT) and scanning electron microscopy (SEM). A range …
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The role of actomyosin contractility in zebrafish neural tube formation through hollowing.
… it an ideal in vivo model to study hollowing lumen formation. While the role of actomyosin contractility is well understood in primary neurulation, for example through constriction of apical-lateral junctions to mediate neural plate bending, its role in secondary neurulation is less clear. In …
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Angiogenesis and angioregression gene expression analyses in swine corpus luteum
… as well as vascular permeability and vessel lumen formation. During vascular invasion and apoptosis process, the remodelling of the extracellular matrix is essential for the correct evolution of the CL, particularly by the action of specific class of proteolytic enzymes known as matrix …