Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 43 for “"luciferase assays"”.
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Synthesis of internal amide bond short interfering RNAs (siRNAs) and investigation of their gene silencing properties
… in this work included hybridization affinity assays (melting temperature), secondary structure determination (circular dichroism), cell-based luciferase assays, and nuclease stability assays. Melting temperature experiment reveals that localizing a UaU dimer unit within the RNA …
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Amino Acid Residues in LuxR Critical for its Mechanism of Transcriptional Activation during Quorum Sensing
… operon in recombinant <I>Escherichia coli</I>. Luciferase levels present in cell extracts obtained from these strains were also quantified and found to correlate with the luminescence results. Eight strains encoding altered forms of LuxR exhibited a "dark" phenotype with luminescence output less …
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Funktionelle Untersuchungen zum PTPN11-Genprodukt SHP2 und zu PTPN11 Mutanten, die dem Noonan-Syndrom zugrunde liegen
… SHP2-Varianten in vitro mit Hilfe eines SOCS1-Luciferase-Assays. Bei gain of function bot sich der Versuch der allelspezifischen Inhibition von PTPN11-Mutanten mit Hilfe der RNAi-Technik an. Schließlich sollte ein Mausmodell für das NS und das LS etabliert werden, da es zu Beginn dieser Arbeit …
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The role of p90 ribosomal S6 kinases (RSKs) in Steroid signalling
… 3.1(+) and to produce phospho-mimetic mutants. Luciferase assays were used to determine optimal hormone and DNA concentrations, the role of RSKs on SR activity, the activity of the endogenous MAPK pathway and the significance of phosphorylation state. The PMA experiments indicated that in most …
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Phytochemical, Chemopreventive and Antimalarial Activity Evaluation of Five Selected Medicinal Plants from the Cameroonian Flora
… were evaluated using haem polymerisation and the luciferase assays, respectively. The n-hexane and the DCM extracts of C. oligandrus, and the DCM and MeOH extracts of Z. zanthoxyloides were the most active in the luciferase assay causing 18, 21, 34 and 36-fold induction of the level of luciferase …
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Role of plasma membrane ATPase4 in the pathophysiology of pulmonary arterial hypertension
… is independent of canonical Smad signaling. A luciferase reporter construct harboring the PMCA4 3′UTR was engineered to assess post-transcriptional regulation. Luciferase assays demonstrated that TNF-α destabilizes PMCA4 transcripts through specific 3′UTR elements. Cloning and validation of …
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The role of DAXX in the selective autophagy receptor SQSTM1/p62 phase condensation
… redox homeostasis, Reactive Oxygen Species (ROS) assays and Dual-luciferase assays were applied, and results showed that the DAXX-induced p62 phase condensation promotes p62 recruitment of Keap1 and subsequent Nrf2-mediated stress responses. Collectively, this study suggests that cytoplasmic DAXX …
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Untersuchung der ACTH-Sensitivität bei Normalpersonen: Einfluß eines Polymorphismus im ACTH-Rezeptor-Gen
… vorliegt. Bei Transfektionsexperimenten mittels Luciferase-Assays sah man, dass dieser Polymorphismus eine verminderte basale und Forskolin-induzierte Promotoraktivität zur Folge hat. Im Rahmen dieser Arbeit wurde die statistische Verteilung des Polymorphismus bei 1266 gesunden Männern zwischen …
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Identification and characterisation of microRNAs expressed in articular cartilage and osteoarthritis
… array were further validated by subcloning and luciferase assays, resulting in the discovery of ITGA5 as a target of candidate novel 11. The outcomes from this research provide us with an increased understanding of the miRNome of human OA cartilage and could impact upon future drug development …
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Analysis of Cellular Transcriptomic Changes Induced by Merkel Cell Polyomavirus miRNA
… stimulator chemokine, CXCL8. Moreover, dual luciferase assays revealed that SP100 and MAPK10 (a member of mitogen-activated protein kinases (MAPK) family which is involved in regulation of CXCL8 expression) are directly and specifically targeted and downregulated by MCV miRNA. The MCV …
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Investigating the biological roles of the HSPRO genes in Arabidopsis thaliana
… of environmental stress cues, promoter-luciferase assays revealed a diurnal expression pattern of the gene that was driven by the circadian clock. However, phenotypic analysis did not reveal a requirement of HSPRO2 for normal clock modulation. Since stress perception typically causes …
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Regulation of (1,3;1,4)-β-Glucan Synthesis in Barley (<i>Hordeum vulgare</i> L.)
… protoplast transient expression system. Dual luciferase assays based on multiple HvCslF6 deletion constructs revealed the essential promoter fragment driving HvCslF6 expression. This finding was combined with an in silico analysis of putative TF binding sites which allowed the identification …
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Novel RNA-based biomarkers for ovarian cancer – uncovering how LINC01132 is regulated by the tumour suppressor protein, p53
… cancer patients, were unable to activate firefly luciferase expression from a synthetic p53-responsive promoter, reflecting the impact of p53 core domain mutations on its ability to bind target promoters and transactivate gene expression. In silico genome-wide ChIP-seq analysis of differentially …
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The Role of STAT and the Jak/STAT Pathway In Mediating the Effects of Interleukin-6 on StAR Expression
… and StAR protein levels. Electromobility Shift Assays (EMSA) were performed to show that STATs bind to the full STAT site within the StAR promoter region. Various experiments were also carried out in the presence of IL-6 alone or, congruently with either a Jak (AG490) or STAT3 (Piceatannol) …
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MicroRNA targeting in mus musculus and Caenorhabditis elegans
… in larval development in C. elegans. We used luciferase assays to test the efficacy of the seven proposed lin-4 binding sites in the lin-14 3'UTR and to determine if lin-4 is capable of recognizing and mediating repression through them. The wild-type lin-14 3'UTR was compared with mutant UTRs …
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Proteins and instability elements involved in phosphoenolpyruvate carboxykinase mRNA turnover
… to the PEPCK 3'-UTR, we employed RNA-gel shift assays, immunoblocking assays, affinity pull down assays, western blot technique and MALDI-TOF/TOF analyses. The identified proteins bind to AU- and CU-rich sequences and secondary structures found within the PEPCK 3'-UTR. A tetracycline regulated …
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Microrna 21 targets B Cell Lymphoma 2 (Bcl2) Mrna to increase beta cell apoptosis and exosomal Microrna 21 could serve as a biomarker of developing Type 1 Diabetes Mellitus
… cells, which constitutively overexpress BCL-2. Luciferase assays suggested a direct interaction between miR-21 and the BCL2 3’untranslated region. With miR-21 overexpression, PRP revealed a shift of BCL-2 message toward monosome-associated fractions, indicating inhibition of BCL2 translation. …
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Cytokine modulations by type I interferon suppression-negative and NF-κB activation-negative Porcine Reproductive and Respiratory Syndrome Virus
… was measured in coinfection groups, and NF-κB luciferase assays and RT-qPCR were used to determine cytokines modulations. PD-∆135-NLS most significantly attenuated the TNF-α, IL-1β, IL-6, and IL-8 expressions compared to those of wild-type PRRSV. The viability of S. suis was increased, and …
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Mapping the transcriptional landscape of haematopoietic stem and progenitor cells
… factor binding profiles and in vitro luciferase assays using a model cell line. Overall, the work presented in this thesis confirmed known aspects of HSPC biology using single-cell gene expression analysis and demonstrated how in silico approaches can be used to guide in vitro and in …
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Role of the C-terminal domain of the <font face = "symbol">a</font> subunit of RNA polymerase in transcriptional activation of the <i>lux</i> operon during quorum sensing
… of transcription of the <i>lux</i> operon via luciferase assays in recombinant <i>Escherichia coli</i>. The mutant RNAPs from strains exhibiting at least two fold increased or decreased activity in comparison to the wild-type were further examined by <i>in vitro</i> assays. Since full-length …
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