Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 39 for “"luciferase assay"”.
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Using a chemical genetics approach to dissect the nitrogen signalling pathway in Arabidopsis
… in conjunction with Arabidopsis lines expressing luciferase reporter genes in roots. This semi-hydroponic platform allows roots to be exposed to a variety of nutrient treatments in a 96-well plate format suitable for chemical genetic screens. This was combined with a newly developed ‘ice capture’ …
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ROLE OF MICRORNAS IN THE PATHOGENESIS OF ATOPIC DERMATITIS
… By using target prediction software and luciferase assay, we identified SOX6 and CASP7 as novel bona fide targets of miR-335. Both SOX6 and CASP7 were found to be overexpressed in AD skin. SOX6 was shown to regulate keratinocyte differentiation, while CASP7 may regulate skin immune …
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Regulation of the kRAS Promoter in Pancreatic Cancer by Proteins and Small Molecules
… system with plasmids in HEK-293 cells by luciferase assay, and in complex in vitro milieus within pancreatic cancer cell lines by RT-qPCR. Protein changes were evaluated by western blotting. TF binding to the kRAS promoter was predicted based on consensus binding sites by online tools, and …
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Self-Assembled Gold Nanoplexes for Cancer-Targeted siRNA Delivery
… and the results were analyzed by GLuc assay, TB assay, and Luciferase assay. In terms of RNA delivery, the Au nanoplexes AuPEI/RNA/PEI exhibited excellent gene silencing efficiency (or causing severe cell death) in the absence of the serum during the transfection. In terms of targeted …
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Kombinierte Freiland- und Laboruntersuchungen zur Wirkung endokriner Schadstoffe auf Fische
… in Kombination mit einem weiteren Biotest (ER Luciferase Assay) mit In situ-Befunden an exponierten Regenbogenforellen und mit chemischer Analytik verglichen. Vielfach ist die hormonelle Wirkung von Einzelsubstanzen auf Fische nicht bekannt, so dass parallel hierzu ein Fischtestsystem …
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Variations in bacterial adenosine triphosphate values due to genus and environmental conditions
… using the bioluminescence (luciferin-luciferase) assay. Environmental factors (temperature, atmosphere, pH, aeration, and phase of growth), as well as differences among genera and species, were studied in relation to their effect on cellular ATP. Variations for each of the …
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The Role of the ORB2 RNA-binding Protein in Post-transcriptional Regulation
… to zygotic transition (MZT). Using a dual luciferase assay, I show that ORB2 confers translational repression on a target mRNA and I map this repressive function to the C-terminal ZZ domain of the ORB2 protein. Next, I identify over 200 proteins which interact with ORB2 in the early embryo. …
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Phytochemical, Chemopreventive and Antimalarial Activity Evaluation of Five Selected Medicinal Plants from the Cameroonian Flora
… on ethnomedicinal use and literature review. Bioassay-guided isolation of active components from the active plants was performed with the aim to scientifically validate their folklore usage. The plant materials were dried, ground, and Soxhlet-extracted, successively, with n-hexane, …
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Regulation of Notch Ligands Dll1 and Jag1 by Pax6 during cortical development
… by Pax6. Bioinformatic screening and analysis by luciferase assay suggests that Jag1 is a likely candidate to be a direct target of Pax6.
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Investigation of the effects of Mechanical Strain in Human Tenocytes
… as appropriate. qRT-PCR and a cell based luciferase assay were used to assess RNA and TGFβ activity respectively. The prolonged application of 5% cyclic mechanical strain in a 3D culture system induced an anabolic response in protease and matrix genes. In most genes changes in gene …
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Seed dormancy and germination of northern red oak
… AMP) levels were measured using the luciferin-luciferase assay). Energy charge (EC) increased during stratification at 2 weeks when germination was low. Thereafter EC decreased before increasing at 8 weeks of stratification. This second rise in EC was concurrent with an increase in …
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A Network Approach to Understanding miRNA Regulation in Adipose Tissue
… in the leptin 3'-UTR that were confirmed with luciferase assay validations. miR-29 gain and loss-of-function modulated leptin mRNA and protein levels in primary adipocytes, and miR-29 abundance inversely correlated with leptin levels in adipose tissues. This work represents the only …
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The Role of Extracellular ATP in Dictyostelium discoideum Growth and Development
… In these experiments it was found, using the luciferase assay, that the cells condition their media with ATP during axenic growth and development. Treatment of cells with apyrase, a potato enzyme known to degrade ATP, did not affect the rate of growth, but led to a delay in the appearance of …
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The Mechanism of Palmitate Decreased Glucose Transpoter 4 Expression in C2 Myotubes
… 14 4. 2-deoxyglucose uptake 15 5. RT-PCR 15 6. Luciferase assay 17 7. Palmitate oxidation 17 8. Pyruvate oxidation 18 9. Pyruvate assay 18 10. Transfection 19 Ⅲ. 결과 21 A. 고농도 지방산의 지속적 노출에 의한 인슐린 저항성 및 GLUT4 양적 감소 21 1. ZDF 백서의 근육에서 GLUT4 mRNA 감소 21 2. 인슐린 저항성 및 GLUT4 양적 감소 21 3. GLUT4 promoter …
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Ehrlichia chaffeensis TRP32 is a Nucleomodulin that Regulates Host Gene Expression and Post-Translational Modifications Determine its Localization and Function
… inhibitor carfilzomib. An electromobility shift assay (EMSA) demonstrated TRP32 binding to host DNA via its tandem repeat domain. TRP32 DNA binding and motif preference were further confirmed by supershift assays, as well as competition and mutant probe analyses. Using ChIP-Seq, I determined that …
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Cell Cycle Regulatory Roles of Estrogen Receptor Alpha (Erα) In Breast Cancer Cells
… cell cycle regulated effects of liganded ERα, a luciferase assay was performed and showed that the transcription of target genes such as Progestrone Receptor (PgR) and Trefoil protein (pS2) increased duing S and G2/M phases when ERα is bound to ligand. Additionally, complex formation between …
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Prostate Specific Membrane Antigen (PSMA): Immunoassay Development and Characterization of Transcriptional Regulation
… study is to develop a clinically useful immunoassay for quantitation of serum PSMA and to study the molecular mechanism underlying the upregulation of the PSMA gene after androgen deprivation therapy. Surface Enhanced Laser Ionization/Desorption (SELDI) ProteinChip® mass spectrometry was used …
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Nucleic acid clamp-mediated transcriptional and translational modulation of oncogenes via the stabilization of G-quadruplexes
… corresponding target using electromobility shift assay, electronic circular dichroism and DMS footprinting. Further studies include in vitro cytotoxicity, luciferase assay, fluorescent microscopy, ChIP-PCR, qPCR and western blot were used to evaluate the pharmacological effect of clamps on tumor …
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STAT5 regulierte differentielle Genexpression in prä-B-leukämischen Zellen
… aktiven STAT5 Proteins (PRL-Promoter, Luciferase Assay), (3) klonogene Wachstumstendenz und hohe zelluläre Adhärenz und (4) Hemmung des Zellwachstums der BaMRS Zellen und Inhibition der STAT5 Phosphorylierung durch AG490, einen spezifischen JAK-Kinaseinhibitor. <br>Diese Charakteristika …
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Angiotensin II-mediated Regulation of the Human Angiotensin II Type 1 Receptor Gene
… In vitro cell-free RNA degradation assays did not show any increase in the rate of degradation of in vitro transcribed RNA in the presence of cytoplasmic extract from cells treated with Ang II, which suggested that hAT1R steady state mRNA levels may not be mediated by changes in mRNA …
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