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Showing 1 to 20 of 453 for “"luciferase"”.
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Studies using luciferase-expressing Leishmania donovani parasites
… (LV82 and 200011), which express different luciferase genes and thus emit light of different wavelengths (red or green) were assessed for their ability to act in drug screening studies. We assessed if the insertion of the luciferase gene into L. donovani parasites altered their …
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On-line fermentation monitoring via recombinant firefly luciferase
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1995.
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Bacterial Luciferase: Studies of Proteolytic Inactivation and Ligand Binding
The mechanism of proteolytic inactivation of luciferase was examined. Proteolytic inactivation in vitro was found to be concomittant with: (1) the loss of enzymatic activity; (2) altered enzyme affinity for substrate and product; (3) changes in enzyme secondary structure; and (4) the fragmentation …
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Amino Acid Sequence Studies on the Beta Subunit of Bacterial Luciferase
… acid sequence of the (beta) subunit of bacterial luciferase from Vibrio harveyi were isolated and their amino acid sequences were determined by Edman degradation. The protein was fragmented by digestion with S. aureus V8 protease, trypsin, trypsin after citraconylation, and by treatment with …
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Design and Applications of Split-Luciferase Systems in Vitro and in Cellulo
… Spilt-protein reporters, such as split-firefly luciferase (Fluc), depend upon the conditional reassembly of the genetically fragmented enzyme. The two fragments of Fluc conditionally reassemble and regain activity when protein domains appended to the N-terminal and C-terminal Fluc fragments …
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Active Center Studies on Bacterial Luciferase: Modification of the Enzyme With 2,4-Dinitrofluorobenzene
Bacterial luciferase catalyzes the mixed-function oxidation of a long-chain saturated aldehyde and FMNH(,2) to yield the carboxylic acid, FMN and blue-green light. The enzyme is inactivated by 2,4-dinitrofluorobenzene (FDNB) with an observed second-order rate constant (k(,2(obs))) of 157 M('-1) …
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Entwicklung eines Dual-Luciferase-Reportergen-Assays zum Nachweis der Induktion antioxidativer Enzyme durch Nahrungsbestandteile
… davon wurde in der vorliegenden Arbeit der Dual-Luciferase-Reportergen-(DLR)-Assay zum Nachweis der Induktion der antioxidativen Enzyme Katalase (CAT), zytosolische Glutathion-Peroxidase (GPX1) und Kupfer-Zink-Superoxid-Dismutase (SOD1) entwickelt. Im Zuge dessen wurden drei Säugetierzelllinien …
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Shining light on food microbiology; applications of luciferase-tagged microorganisms in the food industry
… catalysed reactions caused by enzymes termed luciferases. The lux genes responsible for the emission of light can be cloned from one bioluminescent microorganism into one that is not bioluminescent. The light emitted can be monitored and quantified and will provide information on the metabolic …
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The in Vitro Luminescent Reaction of Photobacterium Fischeri: Purification and Properties of Bacterial Luciferase
Made available in DSpace on 2014-12-05T19:38:10Z (GMT). No. of bitstreams: 1 6303319.pdf: 4806985 bytes, checksum: 45977c45e93184fc19de6c35aa0b259d (MD5) Previous issue date: 1962
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Rapid, Quantitative Assessment of Antimycobacterial Water Disinfection based on the Firefly Luciferase Reporter Gene
… for the viability of mycobacteria using firefly luciferase as a reporter gene for disinfection survival studies. Derivatives of M. avium strains MD1 and A5, Mycobacterium smegmatis strain VT307 and Mycobacterium bovis BCG strain Pasteur carrying the firefly luciferase gene (pLUC10) were …
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A Study of the Inactivation in Vivo of the Luciferase in the Luminous Marine Bacterium Beneckea Harveyi
The inactivation in vivo of the luciferase in stationary phase cultures of the luminous marine bacterium Beneckea harveyi was studied in an effort to elucidate its mechanism. Through studies utilizing inhibitors of metabolic energy production, an inhibitor of protein synthesis, and a mutant in …
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Active Center Structure and Sequence Studies on Bacterial Luciferase Utilizing the Essential Cysteine, Protease-Labile Region, and Delta Fragment
… the structure of the active center of bacterial luciferase by exploiting the existence of two well-documented structural features of the (alpha) subunit of the enzyme, the essential cysteine and the protease-labile region, which are thought to reside there. Chemical modification of this cysteinyl …
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Growth of luciferase-transfected BCG in whole blood : use of a novel assay to study immune responses to mycobacteria in children
The existing Bacille Calmette-Guerin (BCG) vaccine offers some protection against TB, but its efficacy varies for unknown reasons. In order to develop an effective vaccine, a comprehensive understanding of protective immunity in adults and children is needed. Studies involving host-pathogen …
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THE EVOLUTION OF BEETLE BIOLUMINESCENCE
… of fireflies and glow-worms; the protein luciferase and the luciferin regenerating enzyme (LRE). A range of parallagous genes for both enzymes were identified in bioluminescent and non-bioluminescent taxa using degenerate primer PCR. Combined with genomic and database sequences phylogenies …
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Engineering Secreted Proteins for Gene Transfer and DNA Vaccination
… diseases. In this work,<em> Renilla</em> luciferase and human glutamic acid decarboxylase (GAD) 65 were engineered for secretion to address some aspects of these issues.</p> <p>Secreted reporter proteins are promising tools to study gene transfer and expression in a non-destructive manner, …
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Transcriptional Regulation Of Adipocyte Function
… white adipose tissue. Next, through a leptin-luciferase animal model, where luciferase is expressed only in adipocytes (see below), the location and timing of embryonic adipose development were determined. Identification of the location and timing led the way to study gene regulation and …
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Development of Bioluminescent Biosensors Monitoring Activity of the Merlin Tumour Suppressor
… study. To address this issue, we leverage novel luciferase technologies to develop and validate 2 split-luciferase biosensor systems that enable accurate quantification of Merlin activity in real time. First, we design an intramolecular biosensor aimed at studying Merlin’s changes in conformation …
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Versatility of M13 bacteriophage in medicine : vaccine storage and cancer diagnostics
… and noting the diffraction pattern. We chose luciferase as a model for a protein-based vaccine, and found several phage-borne peptide sequences with increased affinity to luciferase compared to controls. Two of these sequences, CKLHGTSRC and CTHKNQAC were chosen to form luciferase-encapsulated …
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Using a chemical genetics approach to dissect the nitrogen signalling pathway in Arabidopsis
… in conjunction with Arabidopsis lines expressing luciferase reporter genes in roots. This semi-hydroponic platform allows roots to be exposed to a variety of nutrient treatments in a 96-well plate format suitable for chemical genetic screens. This was combined with a newly developed ‘ice capture’ …
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Cloning and characterization of glycogen synthase from Dictyostelium discoideum
… consensus splice sites for Dictyostelium. The luciferase reporter gene was used to study the transcriptional regulation of glycogen synthase. I defined the cis-acting elements that are required for proper transcriptional regulation of glycogen synthase by using promoter/luciferase fusions of …
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