Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"low copy number"”.
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Simplified Low Copy Number Dna Analysis By Post Pcr Purification
… sensitivity of standard 28 cycle PCR such that low copy number DNA templates (<100 pg DNA) can be analyzed. Full profiles were consistently obtained with as little as 20 pg template DNA without increased cycle number. In mock case type samples with dermal ridge fingerprints, genetic profiles …
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Spatiotemporal modelling in biology: from transcriptional regulation to plasmid positioning
… regulation of the floral repressor FLOWERING LOCUS C (FLC ) in Arabidopsis thaliana and spatial positioning of low copy number plasmids in Escherichia coli. Despite the diversity in biological subjects, my spatiotemporal modelling approach provides a common ground. FLC regulation …
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Stochastic modelling for control and interconnections of biological systems
… volumes within a cell, restricting molecule numbers to the hundreds or even tens. At this scale, reactions are inherently discrete and stochastic. Traditional models based on differential equations and mass-action kinetics fail to capture the behaviour of such systems. In contrast, stochastic …
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Effects of the conserved proteins, Soj (ParA) and SpoOJ (ParB) on chromosome partitioning in Bacillus subtilis
… viable. Since the discovery of the Par system in low-copy number plasmids over 20 years ago, research has focused on characterizing its components: ParA, a DNA-binding ATPase, ParB, a DNA-binding protein that interacts with ParA, and parS, the site bound by ParB. How these proteins function to …
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Analysis of the Nucleioprotein Complexes Essential for P1 Plasmid Partition
… and the same holds true for bacterial plasmids. Low-copy-number plasmids and bacterial chromosomes encode active partition systems to ensure their inheritance within a bacterial cell population. One of the well-studied models of partition is that of the P1 plasmid in E. coli. The partition system …
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The prevalence of toxin-antitoxin systems and their tractability as novel antimicrobial targets
Large, low copy number plasmids, such as those harboring antibiotic resistance genes, often possess plasmid maintenance systems to ensure their persistence and inheritance in a bacterial population. One example is the proteic toxin-antitoxin (TA) system, consisting of a toxin and an antitoxin …
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Investigation of coiled-coil interactions between proteins of the spindle pole body
… achieved using x-ray crystallography, due to its low copy number, large size, heterogeneous composition, and association with the nuclear membrane. However, structural information may be deciphered through a variety of other techniques. Cryo-electron microscopy images have provided a …
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An investigation into the partition functions of the broad-host-range Plasmid pTF-FC2
… stably inherited over many generations despite a low copy number. The pas genes which lie between the repB primase and the repA helicase encode a proteic plasmid stabilisation system and are capable of stabilising the unstable heterologous R1 replicon present on p0U82. The deletion of the pas …
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Acinetobacter baumannii toksino-antitoksino sistemos CheTA antitoksino veikimo mechanizmo paieška /
… (TA) systems, genetic loci abundant in low copy number plasmids and chromosomes, sparked a great interest from scientists. They have been proposed to fulfill a myriad of roles in bacterial physiology, including plasmid stabilization, stress response, persistence and protection from phage …
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Regulation of blaFOX-5b Expression Within Two Different Genetic Backgrounds: Klebsiella pneumoniae and Escherichia coli
… start site of transcription and determine the number of initiated transcripts expressed in the clinical isolates. Four deletion clones were constructed using PCR and represent the entire h/aFOx-5b structural gene plus regions corresponding to 4, 45, 107, 262, 437 or 593 base pairs upstream of …
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Functional dissection of ParB homologue and global regulatory protein KorB of RK2
RK2 is a low copy number plasmid responsible for spread and maintenance of important properties (including antibiotic resistance and degradation functions) among bacteria. Gene expression in RK2 is controlled by cooperativity among four repressors (i.e. KorA, KorB, KorC and TrbA) to tightly …
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Molecular analysis of glycogen phosphorylase-1 gene expression during the development of dictyostelium discoideum
… screening. The gp-l gene was found as one copy or low copy number gene in the <i><b>Dictyostelium</i></b> genome, and an adjacent 22 kilobase pair region was physically mapped. The deduced amino acid sequencing analysis revealed that there were 862 amino acid residues encoded by the gp-1 …
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Sex estimation of unidentified human remains: Concordance between morphological anthropological assessment and DNA analysis
… However, since the DNA concentrations were below the validated dynamic range, these results were suggestive only. DNA profiling confirmed that one case was male but did not provide further clarity on the biological sex of the remainder of cases due to low copy number (LCN) DNA. This study …
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Investigation of Streptomyces promoters
… in a more sensitive reporter system. High copy number promoter probe vectors were constructed and tested. A low copy number promoter probe (which used the Streptomyces penemefaciens pSPN1 origin of replication) was also constructed. The characteristics (copy number, stability and mobility) …
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Single-molecule detection of molecular beacons generated from LDR on thermoplastic microfluidic device for bioanalysis
… bringing Cy5 and Cy5.5 into close proximity to allow FRET to occur. These rMBs were analyzed using single-molecule detection of the FRET pairs (spFRET). The LDR was performed in a Cyclic Olefin Copolymer (COC) microfluidic device equipped with 2 or 20 thermal cycles in a continuous flow format. …
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Fast simulation of stochastic biochemical reaction networks on cytomorphic chips
… ex- pensive Gillespie algorithms and random-number generation for accurate simulations; and, they routinely operate with nonlinear statics and dynamics. Hence, such simulations are extremely computationally intensive and have remained an important bottleneck in computational biology over …
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The mechanism and function of pervasive noncoding transcription in the mammalian genome
… transcription typically leads to short-lived, low-copy number noncoding RNAs (ncRNAs). We are starting to understand the biogenesis and mechanisms regulating the noncoding transcription. However, it is still unclear what's the functional impact of pervasive transcription and the ncRNAs at the …
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Single-Molecule Protein Sequencing (I) and Genetically Dominant mRNA Therapies to Combat Viral Evolution (II)
… central dogma of molecular biology, information flow from DNA to RNA to protein, has been studied for decades as these molecules are critical to cell function and diversity. The advent of polymerase chain reaction (PCR) amplification of nucleic acid was pivotal in advancing the high-throughput …
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Assessment And In Vitro Repair Of Damaged Dna Templates From Forensic Stains
… often intractable to analysis. This may due to a number of factors including a low copy number (LCN) of starting molecules, the presence of soluble inhibitors or damaged DNA templates. Remedies may be available to the forensic scientist to deal with LCN templates and soluble inhibitors but none …
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Molecular epidemiology of M. Tuberculosis transmission in the United Arab Emirates
… polymorphism (RFLP) of 106 isolates revealed low copy number of 156110 which seemed to be related to South Asian sub-population strain. There was evidence of typical mycobacterium species prevailing for primary infections. The data showed increasing rate of infection by atypical mycobacterium …
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