Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"long-term culture"”.
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A NOVEL DROPLET-BASED PLATFORM FOR TUMOR SINGLE-CELL ENCAPSULATION AND METASTATIC MULTICELLULAR CLUSTER MODELLING
… droplet stability, nutrient exchange, and long-term culture capacity. It incorporates inertial focusing and on-chip sample enrichment techniques to enhance cell loading efficiency and reduce variability. Functional analysis revealed the influence of biophysical stress on viability, …
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Viral and Host Genetic Determinants of Hepatitis C Virus Persistence and Interferon Resistance
… of persistent HCV RNA replication in tissue culture. We evaluated HCV/IFN interactions within a long-term culture system of Huh7 cell lines harboring different variants of an HCV genotype 1b subgenomic RNA replicon that differed only at two sites within the NS5A coding region. A replicon with …
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Tissue-engineered liver microreactor as an in vitro surrogate assay for gene delivery
… preventing the extrapolation of successful cell culture results into animals. This phenomenon has also been documented in the specific case of liver where standard hepatocyte culture systems fail to reliably predict the in vivo performance of gene delivery vectors. This is possibly a consequence …
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A novel polymeric microelectrode array for highly parallel, long-term neuronal culture and stimulation
… would be employed to constrain the neuronal culture network connectivity. In order to ensure longterm survival of the cultures, a bottom feeder layer of glial cells would be grown. In addition to high-throughput screening application, the polymeric microelectrode arrays and integrated …
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Synergistic development of an engineered biodegradable theranostic probe and lymph node organ-on-a-chip to study nanoparticle-lymphatic interactions
… a lymphatic vessel on-a-chip that achieved long-term culture of primary human lymphatic endothelial cells (HLECs) under gravity-driven flow. We then developed a lymphatic system on-a-chip, comprised of multiple units that each model the lymph node subscapsular sinus, high endothelial …
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Advancing single cell microbiology and bacteriophage-bacteria interactions using microfluidics and image-based deep learning
… and developing effective therapies. Conventional culture-based assays are often slow, labour-intensive, and limited in their ability to capture the heterogeneous responses of individual bacteria to antibiotics or bacteriophages. This thesis addresses these challenges by developing integrated, …
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Effect of Cellular Positional Identity on Bone Regenerative Capacity for Tissue Engineering
… In addition, the ability of heterotypic co-cultures to reprogramme site-specific Hoxa gene expression also tested. Rat osteoblastic cells from femurs and calvariae were harvested as matched pairs of cultures from 4 male rats. Cells were expanded extensively in medium supplemented with FGF-2, …
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Transcription factor regulation of T helper subset function
… vitro, and that PU.1 expression decreases during long-term culture. To examine a role for PU.1 in Th9-independent immunity we examined a model of multiple sclerosis termed experimental autoimmune encephalomyelitis (EAE). Mice that lack PU.1 expression in T cells (Sfpi1lck-/- mice) demonstrated …
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Impacts of ocean acidification on fitness and chemical communication in a model marine invertebrate, Nereis succinea
… organisms and few studies have incorporated longJterm exposure to OA conditions. Additionally, the impacts associated with OA are variable between species. The presented research shows long-term culture (4-6months) in near future OA conditions (CO₂ enriched to pH 7.8) delays metamorphosis in …
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Three-dimensional Hyaluronan-based Hydrogels Designed for Breast Cancer Spheroid Formation
In vitro culture of cancer cells is traditionally performed with two-dimensional (2D) culture, which fails to model the in vivo tumour cell phenotype and microenvironment. Hyaluronan (HA) is upregulated in breast cancer stroma and thus an excellent starting material in which to culture breast …
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Étude de l’expression et du rôle du ligand du co-stimulateur inductible des cellules T (ICOSL) dans les ostéoclastes humains
… model of fetal monocyte-derived osteoclasts in long-term culture. Inhibition of ICOSL expression by DsiRNA resulted in a significant reduction in the number of multinucleated cells, and in bone resorption, compared with control DsiRNA. The impact of ICOSL on apoptosis is currently being …
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Differentiation of Normal and Cystic Fibrosis Human Lung Epithelial Cells in a Decellularized and Reconstituted Mouse Lung
… primary human bronchial epithelial cells in culture to extend their functional lifespan, and have used these cells to reconstitute lung epithelium in a decellularized lung matrix. For conditional reprogramming, I cultured primary human bronchial epithelial cells derived from patients with or …
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Investigating Human Cell Influence on the Micromechanical Environment of a 3D-Printed Bone Tissue Model
… Current models, such as 2D in vitro cell culture and preclinical animal models, fail to replicate the physiological metastatic bone environment, leading to high failure rates of anti-cancer drugs and limited predictive accuracy. This study aims to enhance a previously developed 3D-printed …
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Proteomic Analysis of Human Mesenchymal Stem Cell Aging during serial sub-culture
… state. Previous studies have shown that long term culture of hMSCs results in the decrease in proliferation rates, changes in genomic expressions, increase in cell sizes, and other morphological changes. Currently the mechanisms of hMSC aging and biomarkers associated with it have not …
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In Vitro Development of Engineered Lung Tissue
… of a bioreactor that is capable of the long-term culture of whole lung tissue in the laboratory. To validate the bioreactor design, we culture native lung tissue for up to 7 days and demonstrate that the bioreactor enables the maintenance of cell viability, cellular differentiation …
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Primary neuronal culture of Locusta migratoria for construction of networks on microelectronic recording devices
… of synaptogenesis and signal transmission over a long time period and at many recording sites simultaneously, it is desired to couple those networks with extracellular recording devices like field-effect transistors or metal electrode arrays. The present work describes a protocol for a primary …
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Development of a hydrogel-based microfluidic model of the lymph node
… models do not easily permit us to follow events longitudinally or to manipulate the environment, while currently there is a lack of in vitro platforms that recapitulate the LN complexity to study its role in response to antigens and in disease. First, I have tried to adapt an existing PDMS-based …
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Characterisation of the effect of stress on nitrogen metabolism in the commercially important agarophyte, Gracilaria gracilis
… nitrate reductase and glutamine synthetase. Long term culture of G. gracilis in nitrogen replete and lacking conditions indicated that nutrient limitation causes a reduction in intracellular nitrogen and nitrogen protein stores such as phycoerythrin. When various sources were introduced to …
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Real Time RT-PCR for Direct Detection of Viable Mycobacterium Avium Subspecies paratuberculosis in Chron's Disease Patients and Association of Map Infection with Downregulation in Interferon-Gamma Receptor (INFG1) Gene in Crohn's Disease Patients
… This theory has been strengthened by recent culture of MAP from breast milk, intestinal tissue and Blood from patients with active Crohn's disease. Culture of MAP from clinical samples remained challenging due to the fastidious nature of MAP including its lack of cell wall in infected …
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Characterization of Thy-1 expression on human hematopoietic cells
… precursor cells including those required for long-term reconstitution of hematopoiesis in vivo. The property of one of these monoclonal antibodies, referred to as 5E10, that was raised following immunization with a human erythroleukemia cell line, HEL, was chosen for more detailed studies. …
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