Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 38 for “"long-read sequencing"”.
-
Genomic Context of microRNAs: Integrating Deep Learning with Capture Long-Read Sequencing
… γονίδια με δεδομένα CLS (Capture Long-read Sequencing) από την έκδοση Gencode 2024 για τον προσδιορισμό των TSS των miRNAs. Χρησιμοποιώντας ένα υψηλό επίπεδο εμπιστοσύνης 0,9 με μέγεθος πα- ραθύρου 66 kb για τη μεγιστοποίηση της ειδικότητας των δεδομένων, κατέστη δυνατός ο …
-
Sensitive detection of complex and repetitive structural variation with long read sequencing data
Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2022-04-06 without embargo terms
-
Engineered Replicases, Synthetic DNA, and Long-Read Sequencing Quality Control for Adeno-Associated Virus Production
… medium-dose, and three AAV-based products are already approved by regulatory authorities. However, rAAV are challenging to produce, and rAAV-based therapeutics are among the most expensive medicinal products. AAV is a non-pathogenic dependovirus with a bi-phasic lifecycle, and rAAV production …
-
Exploring the genetic landscape in cutaneous T-cell lymphoma with short- and long-read sequencing
… in cutaneous T-cell lymphoma with short- and long-read sequencing<br /> Carsten Hain<br /> #### Abstract Cutaneous T-cell lymphoma (CTCL) are a group of diseases with heterogenous genetic alterations. In general, there are recurrent copy-number variants and rare, single nucleotide variants …
-
Computational methods for the detection of somatic structural variants in cancer genomes using long-read sequencing
… method for the analysis of somatic SVs using long-read whole genome sequencing data from tumours and matched normal samples. SAVANA employs machine learning to distinguish true somatic SVs from germline events and noise. Additionally, I establish best practices for benchmarking SV detection …
-
A pan-genomics approach linking genotype and phenotype in the mega-pest, Helicoverpa armigera
… but remains challenging. Recent advances in sequencing technologies, specifically long-read sequencing, have dramatically improved the resolution of the genetic information able to be gained from individuals. This information enables the application of pan-genomic approaches (which are …
-
A pan-genomics approach linking genotype and phenotype in the mega-pest, Helicoverpa armigera
… but remains challenging. Recent advances in sequencing technologies, specifically long-read sequencing, have dramatically improved the resolution of the genetic information able to be gained from individuals. This information enables the application of pan-genomic approaches (which are …
-
Functional genomics of developmental disorders
… microarrays to those generated from genome-wide long read sequencing data, and provide evidence that long read sequencing is a reliable and scalable method to profile 5-methylcytosine for DNA methylation signature-based classification. Overall, my work emphasises the need for scalable, …
-
NANOPORE SEQUENCING AS A NEW TOOL TO EXPLORE COMPLEX TRANSCRIPTOMES
Long-read sequencing marked a revolution in the field of transcriptomics by helping to resolve isoform structure, unannotated splicing variants, complex loci and repetitive regions and proposing as a method for RNA modifications detection. This versatility permits its application to different …
-
Computational Methods for Haplotype-aware De Novo Genome Assembly from Long Reads
… different haplotypes. Over the last few years, long-read sequencing technologies such as Pacific Biosciences and Oxford Nanopore Technologies, have greatly improved haplotype phasing and genome assembly because of the tremendous advantages in terms of read length. Nevertheless, the vast majority …
-
Integrative Genomic Approaches for Plant Trait Discovery, Fungal Pathogen Identification and Taxonomy
… identification. This dissertation integrates long-read sequencing, metagenomic approaches, and genome-based classification to address these limitations. First, long-read resequencing of 29 food-grade soybean and edamame genotypes enabled discovery of structural variants (SVs) associated with …
-
Scaling short read de novo DNA sequence assembly to gigabase genomes
The recent advent of massively parallel sequencing technologies has drastically reduced the cost of sequencing, sparking a revolution in whole genome de novo sequencing. However, these new technologies sample much shorter segments of DNA, called short reads, than conventional but more costly long …
-
Computational methods for single cell RNA and genome assembly resolution using genetic variation
… the recent higher throughput and lower cost of long read sequencing has revolutionized our ability to create reference quality genomes and has revitalized the assembly community. Now, massive efforts are taking place in the Darwin Tree of Life project and the Earth Biogenome project to create …
-
A gene-space de Bruijn graph framework for improved genotyping of antimicrobial resistance genes
… This typically involves assembling sequencing reads and annotating the assemblies for known AMR determinants. Long-read sequencing technologies have greatly improved the completeness of bacterial genome assemblies, allowing for more accurate detection of AMR. However, even with …
-
Expanding the genome editing toolbox for biomedical applications
… editing in mice. One such strategy is CRISPR-READI, which combines adeno-associated virus (AAV)-mediated gene delivery with electroporation of Cas9-RNPs. The AAV delivers transgenes (up to 4.7 kb) as ssDNA to the zygotes, which is followed by electroporation of Cas9-RNPs. This results in …
-
Rarity, cause or consequence: comparative landscape genomics of six lichenized fungi with contrasting range sizes, life histories, and morphologies
<p>Lichens are long-lived, sessile symbiotic assemblages of fungi, algae, and bacteria, often exhibiting a high incidence of rarity. As an emergent trait, rarity encompasses the complexities of a species’ life-history traits and its environment. Using a comparative framework, we examined the …
-
Quorum sensing in the genus Hafnia - in vitro and in silico investigations
… explored using a variety of in vitro approaches. Long-read sequencing of Hafnia spp. isolates was performed and a variety of basecalling and assembly tools were benchmarked in Chapter with the resulting assemblies being used to augment the pangenomic and phylogenomic investigation of the genus …
-
Integrating bioinformatic approaches to promote crop resilience
… become even greater. Natural genetic variation, long used by traditional plant breeders, holds great promise for adapting high performing agronomic lines to these stressors. Yet, efforts to bolster crop plant resilience using wild relatives have been hindered by time consuming efforts to develop …
-
The broken genome: Merging cutting edge technologies for a molecular understanding of the Flock House virus defective interfering particle.
… the establishment of chronic infections, may prolong the host’s infectious period, and may even be exploited as antiviral therapies or vaccines. Therefore, understanding the mechanisms of how DI-RNAs are formed and what roles they play in infections is important. I have characterized the process …
-
Caste-Specific Expression Of Coding And Noncoding Genes In Ant Brains
… by substantial changes in gene expression. Long untranslated transcripts (lncRNAs), are one means by which epigenetics asserts control over gene regulation in a variety of organisms, including Harpegnathos. Technologies for better annotation and functional classification of lncRNAs have …
Page 1 of 2