Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 42 for “"localization studies"”.
-
Order Reduction, Identification and Localization Studies of Dynamical Systems
In the third and final part of the thesis, a new technique based on Green!s function method is proposed to identify the dynamics of practical bolted joints. By modeling the difference between the dynamics of the bolted structure and the corresponding unbolted one, one constructs a nonparametric …
-
Localization studies of sister-chromatid cohesion proteins MEI-S332 and RAD21 in Drosophila
… division. In this thesis, we have analyzed the localization of two cohesion proteins in Drosophila, MEI-S332 and RAD21. MEI-S332 localizes specifically to the centromere from prometaphase to the metaphase-anaphase transition in mitosis, and from prometaphase I to the metaphase II-anaphase II …
-
Investigation of the expression of DMC1, a meiotic gene, in trichomonas vaginalis
… cells. However, relative levels are unclear. Localization studies were done in T. vaginalis using immunofluorescence against Dmc1 protein with an HA tag. These studies showed that recombinant Dmc1 remained in the cytoplasm upon treatment with DNA damaging drugs. Additionally, T.vaginalis Dmc1 …
-
A study of localized failure modes in brittle materials
Localization studies arise from the need to accurately model the behaviour of materials which exhibit instabilities due to strain softening or geometric effects. Localization in finite element modelling of brittle materials such as concrete and rock is a relatively unexplored area in computational …
-
Amino Acid Residues Implicated in the Interaction of Melanocortin Ligands and Their Receptors: A Study of MC2R Selectivity
… <em>Silurana tropicalis</em> MC2R and completed localization studies with this system with MRAP using CHO-K1 cells.</p>
-
Isolation and partial characterization of cDNAs encoding polypeptides that interact with the capB gene products of Dictyostelium discoideum
… with the results of previous mutation and localization studies, suggest a role for these polypeptides in mitochondrial function. Moreover the isolation of cDNAs encoding CABP1B and the detection of interactions between polypeptide 101 and the capA gene products may imply that p34/31 have …
-
Towards understanding of plasmodesmal association and regulation using Plasmodesmata-Located Protein 5 as a model
… functional analyses was taken. Subcellular localization studies of the mutant constructs fused to fluorescent proteins revealed that while the presence of transmembrane domain (TMD) is important for plasmodesmal membrane association, no specific sequence within the TMD is required for its …
-
Functional Analysis of Wild-Type LGN and T450 Mutants
… T450 on LGN function; specifically, additional localization studies of T450A and T450D mutants will be conducted and compared to that of wild-type LGN. Characterization of LGN function relative to the phosphorylation status of T450 could lead to the development of novel treatments for breast …
-
The DNA Transfer Region of Bacteroides Conjugative Transposons Tc(r)Em(r)DOT and Tc(r)ERL
… the transfer proteins have been used in protein localization studies to show that these gene products are localized to the cell membrane. The antibodies have also helped to demonstrate that a mutation in one of the transfer proteins results in increased production of the other transfer proteins. …
-
Cloning, High-Level Expression, and Characterization of Escherichia Coli Cytochrome B(562)
… of the coding sequences for the mature protein. Localization studies on the overexpressed protein revealed that cytochrome b$\sb{562}$ is translocated to the periplasmic space. Similar experiments on cytochrome b$\sb{562}$-M7L (a point mutant of cytochrome b$\sb{562}$ in which one of the heme …
-
Structural and functional studies of heme binding proteins toward the understanding of malaria
… often utilized in biological work to track the localization of proteins. Herein, we report on the novel heme-binding properties of both proteins. We have identified a novel function of mRuby2, a commonly used fluorescent probe. Upon incubation with heme, the fluorescence of mRuby2 decreases, …
-
Mechanistic insights into the PARP10-mediated ADP-ribosylation reaction and analysis of PARP10's subcellular localization
… general dependent on the presence of a nuclear localization signal (NLS) or a nuclear export sequence (NES) in the cargo protein. It was shown previously that PARP10 possesses a functional NES and accumulates in the nucleus if nuclear export is inhibited. Since PARP10’s molecular weight exceeds …
-
Complementation of the salt sensitive S. cerevisiae mutant cch1 with an A. thaliana cDNA library and analysis of isolated genes
… were analyzed by Northern blot detection, and localization studies with GFP fusion protein were performed. In addition fragments from the promoter regions of several cDNA clones were fused to the luciferase and the beta-glucuronidase gene and integrated into the Arabidopsis genome. The obtained …
-
Gene expression associated with drought tolerance in Xerophyta viscosa Baker
… and assumes its full physiological activities. Studies on changes in gene expression associated with dehydration stress tolerance were conducted. A cDNA library constructed from m RNA isolated from dehydrated (85%, 37% and 5% relative water content) X. viscosa leaves, was differently screened. …
-
Characterization of the histone deacetylation activity of the yeast silencing gene SIR2
… activity, but show no sign of defect in vivo. Localization studies of the mutants suggest that the enzymatic activity of Sir2p is necessary for the SIR complex to localize to the telomeres although it is not necessary for Sir2p to localize to the rDNA. The results suggest that while the …
-
The characterization of cysteine protease 4 and superoxide dismutase 6 in Trichomonas vaginalis
… recognized by the CP4 antibody for the protein studies. Iron did not regulate expression of TVAG_355480 and TVAG_467970 at the transcriptional but possibly at the post-transcriptional level. Our results also suggested that processing of recombinant TVAG_467970 occurred through cleavage by …
-
Characterization of PCSK9-mediated LDLR Degradation in Hepatic and Fibroblast Cells
… in humans. Similar to previous studies, data obtained in my project has established that PCSK9 was capable of promoting robust LDLR degradation in liver-derived cell lines; however, minimal effects on LDLR levels were detected in several lines of fibroblast cells despite normal …
-
Role of Cholesterol 24-Hydroxylase in Hippocampal Long-Term Potentiation
… has been revealed by calcium imaging studies in neurons cultured from wild-type and 24-hydroxylase knockout embryos. Neurons from mice lacking 24-hydroxylase have specific defects in N-methyl-D-aspartate (NMDA) receptor function, a subtype of ionotropic glutamate receptor essential for …
-
Study on the Physical and Functional Interaction between Fortilin and Peroxiredoxin I
… co-immunoprecipitation, direct binding and co-localization studies in cultured cells as well as animal tissue and demonstrated that FT stabilizes Prx I in U2OS cells by preventing its proteasomal degradation. Furthermore, our results showed that wild type FT, but not a point mutant of FT that …
Page 1 of 3