Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"localisation microscopy"”.
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Three-Dimensional Single-Molecule Localisation Microscopy and Visualisation Techniques
… (DH-PSF) and 3D single-molecule light field microscopy (SMLFM) are techniques which have an increased depth of field (4 µm and 8 µm respectively) and are capable of isotropic resolutions of 25 nm (DHPSF). This makes 3D SR highly compatible to investigate biological processes such as …
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Applying single-molecule localisation microscopy to achieve virtual optical sectioning and study T-cell activation
Single-molecule localisation microscopy (SMLM) allows imaging of fluorescently-tagged proteins in live cells with a precision well below that of the diffraction limit. As a single-molecule technique, it has also introduced a new quantitative approach to fluorescence microscopy. In the Part A of …
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Super-resolution Microscopy: Novel Developments and Optimisations
… and optimisation of a multifunctional localisation based super-resolution microscope at Cambridge Advanced Imaging Centre. The microscope is optimised to perform single and dual-colour imaging with high localisation precision and accuracy. Moreover, three-dimensional imaging capability …
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Investigating the role of protein aggregation in neurodegenerative diseases using super-resolution imaging
… method is described combining single-molecule localisation microscopy and an alpha-synuclein aggregate specific aptamer to characterise surface immobilised aggregates. This method is validated on an in vitro alpha-synuclein aggregation time course, before being applied to the imaging of …
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Advancing Fluorescence Microscopy Techniques for Volumetric Whole-cell Imaging
… whole-cell imaging based on fluorescence microscopy. The first project establishes a robust single-molecule localisation microscopy (SMLM) pipeline for volumetric imaging in the whole cell using the double-helix point spread function (DH-PSF) approach. The practical challenges associated …
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Development of Three-Dimensional Super-Resolution Imaging Using a Double-Helix Point Spread Function
Single-molecule localisation microscopy (SMLM), has allowed for optical microscopy to probe biological systems beyond the diffraction limit. The intrinsic 3D nature of biology has motivated the development of 3D-SMLM with novel techniques, including the double-helix point spread function (DHPSF). A …
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Synthesis and characterisation of peptide-based probes for quantitative multicolour STORM imaging
… imaging by Edward Taylor Current single molecule localisation microscopy methods allow for multicolour imaging of macromolecules in cells, and for a degree quantification on molecule numbers in one colour. However, that has not yet been an attempt to develop tools capable of quantitative imaging …
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Development of Novel Labelling Strategies for Imaging in Nanoscale Topography
… systems on nanometer scales. 3D single molecule localisation microscopy (3D-SMLM) techniques such as the double helix point spread function (DHPSF) enable the visualisation of these systems on the required spatial scales and take into account the inherently 3D nature of biology. However, the …
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Molecular analysis of GerP and spore-associated proteins of Bacillus cereus
… was to use molecular genetic and fluorescence microscopy techniques to characterise the location and function of the GerP proteins in Bacillus cereus 14579. The GerP proteins have been identified from mutagenesis studies across the Bacilli as being implicated in spore germination, most likely …
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Super-resolution Imaging of Chromatin and Functional Nuclear Architecture
… Y loops and chromosome masses. A single molecule localisation microscopy technique was optimised for imaging within the nucleus, with both 3D and dual-colour capability. Then, the Y loops were imaged at super-resolution, and a specialised clustering protocol was developed for quantification. This …
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Single Molecule Localisation with Fourier Light Field Microscopy
… the temporal resolution of 3D Single Molecule Localisation Microscopy (SMLM). This technique combines the super-resolution capability of SMLM with the principles of parallax; multiple perspective views of photo-switchable fluorophores are captured using a lenslet array in the Fourier plane of a …
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Advances in volumetric super-resolution microscopy and single-particle tracking
Single-molecule localisation microscopy (SMLM) has enabled optical microscopy to probe biological structures and processes at the nanometer scale. To accommodate the inherently 3D nature of biological structures, 3D-SMLM techniques---such as the astigmatic and double-helix point spread functions …
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Optical imaging methods for the study of disease models from the nano to the mesoscale
… below the diffraction limit. Single-molecule localisation microscopy (SMLM), which circumvents the diffraction limit by separating fluorophore emission in time to localise individual molecules in space with ∼20 nm precision, was thus implemented to study a-syn in purified synaptic boutons. A …
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Single-molecule orientation estimation using a polarisation camera
… that are typically used in single-molecule microscopy. This work also includes the development of optical simulation software to generate realistic single-molecule polarisation camera images. This simulation software is then used to validate and test the limits of the data analysis software …
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Investigating transcription factor dynamics and chromatin environment during Notch response
… in real time, using in vivo Single Molecule Localisation Microscopy. Analysis of the behaviour of these molecules revealed that Notch activation led to an increase of bound Su(H) molecules, as well as an increase of the fraction of Su(H) molecules with an exploratory behaviour. Near a target …
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Methods for optical microscopy beyond conventional limits
Optical microscopy seeks to produce accurate and precise enlarged images of an object. It is highly desirable, particularly in live-cell biological microscopy, that the observation process does not perturb the structure, nor interfere with the dynamics, of the sample. These requirements are at odds …
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Synthetic ground truth of biological shapes — Simulating variable Nuclear Pore Complexes for Microscopy
… cytoplasm and an important reference standard in microscopy: it is intrinsic to cells, has a high copy number, it is massive and stereotypically arranged. The average architecture of NPC proteins has been resolved with pseudo-atomic precision, however, observed NPC heterogeneities, such as varying …
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Quantitative super-resolution imaging of cell polarity proteins using DNA-PAINT
Knowing the localisation and spatial organisation of proteins is crucial for understanding their function. The development of super-resolution imaging has improved our ability to garner this information, but counting individual molecules in densely-packed assemblies is still challenging. DNA-based …