Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"local protein synthesis"”.
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FMRP dependent synaptic delivery of messenger RNA
… In chapter 1, the molecular mechanism of local protein synthesis and synaptic mRNA delivery and its roles in Fragile X syndrome and learning and memory are introduced. In chapter 2, my data demonstrated that FMRP can facilitate mRNA deceleration and localization in dendritic spines upon …
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Spontaneous Neurotransmission as a Driver of Calcium Signaling
… that maintain synaptic homeostasis and suppress local protein synthesis.
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mRNA stability in soma and neurites of cultured neuronal cells
Neurons rely on mRNA localization and local protein synthesis in neurites to regulate synaptic plasticity, axonogenesis and neural signaling. The local regulation of mRNA stability in neurites is poorly understood. To determine mRNA decay rates, we analyzed the subcellular transcriptomes of neural …
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Targeting troubled translation : investigating novel therapeutic targets in mouse models of fragile X and 16p1 1.2 deletion syndrome
… potential targeted treatments for ASD. Synaptic protein synthesis is essential for modification of the brain through experience and is altered in several genetically-defined disorders, notably fragile X (FX), a heritable cause of ASD and ID. Neural activity directs local protein synthesis via …
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Axonal Translation and Links to Neuropathies
… regulation of the axonal proteome by local protein synthesis (LPS) plays a critical role in neuronal wiring and axon survival, raising the intriguing possibility that some neurological disorders involve LPS dysfunction. To visualise LPS in situ, I optimised multiple imaging techniques …
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Fragile X Mental Retardation Protein Induces Synapse Loss Through Acute Postsynaptic Translational Regulation
… cause of FXS is loss of the function of a single protein: the Fragile X Mental Retardation Protein (FMRP). FMRP is an RNA-binding protein that plays a complex role in translational regulation. FMRP may be an important regulator of dendritic protein synthesis, which occurs at or near synapses in …
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Repulsive cues and signalling cascades of the axon growth cone
… in the literature regarding the need for protein synthesis in the growth cone in response to external guidance cues. My results resolve the conflicting data in the literature on Semaphorin-3A signalling, where differing labs had shown that inhibiting protein synthesis either blocks or has …
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Role of mGluR5 and FMRP in mouse primary somatosensory cortex
… Genes encoding glutamate receptor associated proteins have a high incidence of mutation in cognitive disorders, especially X-linked mental retardation (MR)(Laumonnier et al., 2007). MR has long been associated with altered cortical connectivity, particularly dendritic spine dysgenesis. There …
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RNA helicase MOV10 is essential for gastrulation and normal CNS development
… These synaptic contacts are also the sites of local protein synthesis, which is required for long-term synaptic plasticity underlying learning and memory. The Fragile X Mental Retardation Protein (FMRP) functions as a translational repressor and is critical for fine-tuning local protein …
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The Role of Ubiquitin-Proteasome-Mediated Proteolysis in Long-term Synaptic Plasticity
… have shown that new gene expression and new protein synthesis are essential for the synaptic changes that give rise to long-term memory. More recent studies indicate that proteolysis by the ubiquitin-proteasome pathway (UPP) plays a critical role in long-term synaptic plasticity, and …
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Development and application of optical microscopy tools for the study of axon guidance
… pathways are initiated, one of which induces local protein synthesis (LPS), a key process to enable rapid navigational responses to guidance cues. Impairments of axon guidance and LPS are associated with several neurological disorders. In this thesis, state-of-the-art optical microscopy-based …
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Biochemical Studies of the ER Resident Kinase PERK and Implications for Local Translational Control
The unfolded protein response (UPR) is a highly conserved protein quality control mechanism that becomes activated in response to misfolded protein accumulation in the endoplasmic reticulum (ER) resulting in ER stress. The ER resident kinase protein kinase R (PKR)-like endoplasmic reticulum kinase …
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The role of the RNA-binding protein FUS in axonal organisation and disease
The role of the RNA-binding protein FUS in axonal organisation and disease In this thesis, I discuss the role of the RNA-binding protein Fused in sarcoma (FUS) in axonal organisation and disease. FUS aggregates in forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), and …
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Investigating the TrkB Interactome during Development and in Neurodegeneration
… plasticity. Guidance cues remodel the nascent local proteome in the developing nervous system. Recently, guidance cue receptors DCC, Neuropilin-1 and Robo2 were shown to interact with the translation machinery. Both the protein and mRNA interactions of these receptors help them to define which …
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Commonalities between SMA and ALS: investigation of ribosome heterogeneity and translational defects
… and their association with different proteins, known as ribosome-associated proteins (RAPs). In particular, these proteins can exert a direct role on mRNA selection and translation efficiency. At present, some RNA-binding proteins (RBPs), such as Fragile X Messenger Ribonucleoprotein 1 …