Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"liver sinusoidal endothelial cells"”.
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EFFECTS OF THE LIVER MICROENVIRONMENT ON HUMAN LIVER SINUSOIDAL ENDOTHELIAL CELLS DURING IN VITRO CULTURE
Liver Sinusoidal Endothelial Cells (LSECs) are specialized endothelial cells that play an important role in the metabolic and xenobiotic clearance functions of the liver. LSECs are distinguished by their hallmark characteristics of lacking a distinct basement membrane and containing open pores …
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Investigating Induced Pluripotent Stem Cells for Tissue Engineering and Hepatotoxicity Applications
Induced pluripotent stem cells (iPSCs) can be differentiated into multiple cell types in the body while maintaining proliferative capabilities. The generation of human iPSC-derived hepatocytes (iPSC-Heps) has resulted in a new source for hepatic cells. The current available options for human …
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Optimization of primary endothelial culture methods and assessment of cell signaling pathways in the context of inflammation
… on methods of establishing and studying specific endothelial cell types in vitro for potential applications in establishing functional microvascular architecture. To achieve these objectives, a model system of primary liver sinusoidal endothelial cells (LSEC) was initially studied due to the high …
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Engineering Organoids for Stem Cell Maturation
The liver is the largest internal organ in the body. It is responsible for performing an array of vital functions, including the filtration of blood, synthesis of different molecules, and metabolism of drugs and toxicants. Hepatocytes, or the main liver cell type, perform most of these functions. …
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The Design of Three-Dimensional Multicellular Liver Models Using Detachable, Nanoscale Polyelectrolyte Multilayers
… of three-dimensional (3D) multi-cellular liver models comprised of primary rat hepatocytes, liver sinusoidal endothelial cells (LSECs), and Kupffer cells (KCs). LSECs and KCs in the liver model were separated from hepatocytes by a nanoscale, detachable, optically transparent chitosan and …
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Using single cell transcriptomics to characterise endothelial populations in embryonic haematopoiesis
Haematopoietic stem cells (HSCs) possess the unique ability to reconstitute an animal’s entire haematopoietic system. As such, it is inherently interesting to the fields of regenerative medicine and developmental biology to understand how these cells first form in the embryo. Haematopoietic stem …
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Proteomic Analysis of Three Dimensional Organotypic Liver Models
In vitro liver models that closely mimic the in vivo microenvironment are central for understanding hepatic functions and intercellular communication processes. Bottom-up shotgun proteomic analysis of the hepatic cells can lend insight into such processes. This technique employs liquid …
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The Design and Assembly of 3D Liver Mimetic Cellular Architectures
We report the assembly of three-dimensional (3D) liver sinusoidal mimics comprised of primary rat hepatocytes, human or rat liver sinusoidal endothelial cells denoted as hLSECs and rLSECs respectively, and an intermediate chitosan-hyaluronic acid (HA) polyelectrolyte multilayer (PEM). The height of …
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Cellular and molecular mechanisms of liver regeneration
Improved understanding of how the liver regenerates would be of great value, particularly given the dearth of therapies for end-stage liver disease. Currently, the only effective treatment for total liver failure is transplantation. Such an invasive, costly and specialised intervention is unable to …
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Innovative microphysiological systems for long-term real-time imaging in biomedicine
… and fundamental problem: how to maintain cells or tissues alive out of human body in such a way they could behave like in vivo. So far, identification and selection of pharmaceutical compounds have occurred through standardized protocols based on traditional in vitro cultures, animal …
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Investigating the Interplay between Inflammation and Matrix Stiffness: Evaluation of Cell Phenotype and Cytoplasmic Stiffness In Vitro
… matrix (ECM), signaling molecules secreted by cells into their surroundings, as well as physical interactions between neighboring cells. Cells are able to interact with their surroundings through a number of different mechanisms such as remodeling of the ECM through adhesion, contraction, …